FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469301
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG92800.3758148561070521No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC86700.3511115088844981No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC66050.26748460394257323No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC63140.25569989239869906No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA61310.24828888823193282No Hit
CAGTAAATTACATTTAAGAAATTCTGAGCATTATGGGGGTGTATAGATCG57610.23330489073628527No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC56710.22966013458869533No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC55770.2258533892789903No Hit
ATACACCCCCATAATGCTCAGAATTTCTTAAATGTAATTTACTGAGATCG54550.22091271983447947No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA53270.21572906664679597No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG51210.2073866247978679No Hit
CACTTAATTTTGTTTCTGATCTCACCATCCAAGCCATTCAGATCGGAAGA49920.20216247431965564No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA49050.19863921004365204No Hit
CAAACAGAAAATGGAATTTCACATGCCCAACCCAGAGATCGGAAGAGCGT42390.17166801455148645No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC42270.1711820470651411No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC41930.1698051391871627No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT41860.16952165815346124No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT41540.16822574485654038No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC39920.16166518379087846No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT39810.1612197135950619No Hit
GAAGGCTGTGTACATGACCTCCTTGGACACGGAGATCGGAAGAGCGTCGT39540.16012628675078494No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG39110.15838490325804752No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG38710.15676501163689643No Hit
GTGATCTTAACCTGTGATCCTGTCCATCATCTACTTCAGATCGGAAGAGC38310.15514512001574535No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT37940.1536467202661806No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC36700.14862505624061223No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT36630.14834157520691077No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA36340.14716715378157624No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG35950.14558775945095395No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAAC35930.1455067648698964No Hit
CCGATACAATAAAGAACAAGAACCCATGACCCTAAACAGATCGGAAGAGC35900.14538527299831006No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT35590.14412985699191794No Hit
CCGTGTCCAAGGAGGTCATGTACACAGCCTTCAGATCGGAAGAGCGTCGT34570.1399991333579827No Hit
GAAGTAGATGATGGACAGGATCACAGGTTAAGATCACAAGATCGGAAGAG34340.1390676956758208No Hit
GTCCGGCGATAATAAGCAAACTAGAAGTATAAGGTGACAAGATCGGAAGA34280.13882471193264814No Hit
TGTGATCTTAACCTGTGATCCTGTCCATCATCTACTTCAGATCGGAAGAG33530.13578741514298986No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT32910.13327658313020568No Hit
TGTCACCTTATACTTCTAGTTTGCTTATTATCGCCGGACAGATCGGAAGA32750.13262862648174523No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC32520.13169718879958336No Hit
GATGGGAAGAGCTGGAACACAAGCTGGATTCTAGATCGGAAGAGCGTCGT32110.1300367998879035No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG31770.1286598920099251No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT31040.12570358980132434No Hit
AGAATCCAGCTTGTGTTCCAGCTCTTCCCATCAGATCGGAAGAGCGTCGT30620.1240027035991157No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC30540.12367872527488549No Hit
CTGCGTTGATGGAGACCAGTGAGACTCTGATATCAGATCGGAAGAGCGTC30500.12351673611277038No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC30460.12335474695065528No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA30350.12290927675483873No Hit
GTCTTCCAGTGTGATGATGGTTCCTGCAGGCAGATCGGAAGAGCGTCGTG29800.12068192577575597No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC29640.12003396912729555No Hit
GATATCAGAGTCTCACTGGTCTCCATCAACGCAGAGATCGGAAGAGCGTC29630.11999347183676676No Hit
GCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCAT29300.1186570612493171No Hit
GTCACCTTATACTTCTAGTTTGCTTATTATCGCCGGACAGATCGGAAGAG29150.11804960189138544No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC29090.11780661814821279No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG29050.11764462898609769No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC28440.11517429426384226No Hit
TACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAACA28370.11489081323014085No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA28020.11347340806163364No Hit
GCCTGCAGGAACCATCATCACACTGGAAGACAGATCGGAAGAGCGTCGTG27560.11161053269730989No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA27300.11055760314356167No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC27170.11003113836668757No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA27110.1097881546235149No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG26910.10897820881293936No Hit
CTGGGTTGGGCATGTGAAATTCCATTTTCTGTTTGAAGATCGGAAGAGCG26500.10731781990125949No Hit
TCAAACAGAAAATGGAATTTCACATGCCCAACCCAGAGATCGGAAGAGCG26210.10614339847592497No Hit
TGCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCA26170.10598140931380987No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG26160.10594091202328108No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA26060.10553593911799332No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT26060.10553593911799332No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC25620.1037540583347271No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA25520.10334908542943934No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC25480.10318709626732424No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC25380.10278212336203646No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA25210.10209366942304725No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC25130.10176969109881703No Hit
GTTTAGGGTCATGGGTTCTTGTTCTTTATTGTATCGGAAGATCGGAAGAG25030.10136471819352927No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC24750.10023079405872351No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)