FastQCFastQC Report
Wed 8 May 2024
EGAF00001129400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208523
Total Bases481.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC157870.49203325019019656No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT81190.25304478104099615No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG81060.25263961018823927No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA79120.24659321438555995No Hit
GGAATGAGAGTAAGCGTGAGGCTGGCGGTGCTGGCAGCAGGGTGGGCACG77520.24160649619778324No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT76380.23805345948899231No Hit
GTGAAGACCAAGAACATGAAGATGATGGTGAAGATGAAGATGATGAAGAT72130.22480748930271033No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC69370.2162054004287954No Hit
CAGCACTACCTGTGGGATCGGCTTCCAGGTGCGGCAGCGCTCCTGCAGCA67880.21156151911642834No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC67790.2112810162183659No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC66850.20835131928304707No Hit
GTCTAAGATAATATCGCTTCTGATTCTCTTCTTCTCCATCTTCTTCATCT65630.2045489466648673No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT65140.20302176421986068No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA63910.19918822461300728No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC62840.19585335682493157No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT62710.19544818597217475No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG62580.19504301511941788No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA61680.19223798613879345No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG58380.18195287987650394No Hit
CTTGGTCTCCTTCACATGTGTGGGCTATGCGGTCACCAAGGCCCACCCTG58370.18192171288783032No Hit
CGCAGTGTACCATGCTGTACTCACGCCAGGGGACCACTGAGACCATCGAG58310.1817347109557887No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC56670.17662332481331752No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA56360.17565714816443578No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC56260.17534547827769972No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA56150.17500264140229008No Hit
CTCCCACATCGTACCCAGTGGCCTCGTACTCCTTGGCCTCCCTGGGCTCA56020.17459747054953323No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC55660.17347545895728345No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG54020.16836407281481228No Hit
GGCTTTGAAAAACTGGTACTACTGCTGCTGCTGCTGCTGCTACTGCTGCT52970.16509153900408383No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG52870.16477986911734777No Hit
AAGCTACATTGGTTGCCCATAAATTCTTGATTGTACCAATCTTCCACAAA52130.16247351195550103No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC51520.16057232564641113No Hit
AGGGCCTGTCAGTCTTATTTTGGGTGTGTTCAGTGCATTAGTGGACCTCT51430.1602918227483487No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA50790.158297135473238No Hit
CAGGACCCTAATAGGAGTATTCATACCAGCAGCAGCAGCAGCAGCAGCAG47700.14866653597309418No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG47520.14810553017696929No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG46740.14567450506042812No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA45720.14249547221572045No Hit
CCCCACGATGTCAGAAGGTGCCACAGTCTCATGGAGCATATAATACTGCA45080.14050078494060975No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA44240.13788275789202695No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC44150.13760225499396453No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG43790.13648024340171475No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC42530.13255320282884056No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA42400.1321480319760837No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACC41760.130153344700973No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC41690.12993517578025776No Hit
ACTGATGACACCACAGGATCTCATCAATTACGTGCAGACAGTAGATTTCA41520.12940533697280648No Hit
GGTACAAATTTTCGGGCACTACTTTCGACTACAGACGGTCCTATAATGAG39980.12460562071707137No Hit
GGATCACGGGTGTCTACACCGCCCTGGGCTATGTGCAGGCTTTCCTGGGA38120.1188085608237809No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA37860.11799821911826719No Hit
CACAACCCCATGCTACCAGCAGTCTAGCTGCCAGCCGGATTGCTGCACCT37160.11581652991111487No Hit
CCAATACACATTACTGACATGTATGCCCACCTGACCTGCACCCACTCATG36830.11478801928488591No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT36600.11407117854539299No Hit
CATCACTGGCTGCATATATGGCATCATTTGGTAATTGCTAGCTTCCAAAA36590.1140400115567194No Hit
GGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGC36560.1139465105906986No Hit
GGAACCCCAGAAAAGACCTTGCCCCTTCTGTCCCTGCAGATGACGTCCAC36050.11235699416834474No Hit
GCGGACGAACAGGGTGTAGGAGGTTTGGGTGAAGGCGGGGGCGTTGTCAT35920.11195182331558788No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT35730.11135965053078939No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA34450.10737027598056802No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC33780.1052820877394365No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG32890.10250822574748568No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC32130.10013953460829172No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)