FastQCFastQC Report
Thu 9 May 2024
EGAF00001129402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478120
Total Bases521.7 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC172930.49719388635239725No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT96420.277218727358458No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA90070.258961737950387No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC82530.23728335997607905No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG74610.214512437753729No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC74480.21413867261624095No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC73100.210171011925983No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC65060.1870550757305671No Hit
GTGAAGACCAAGAACATGAAGATGATGGTGAAGATGAAGATGATGAAGAT64370.18507124538543812No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG62300.17911975435005117No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA61170.17587087277034721No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA61120.1757271169482364No Hit
GTCTAAGATAATATCGCTTCTGATTCTCTTCTTCTCCATCTTCTTCATCT59450.17092567248973584No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT59230.17029314687244831No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG56510.16247283014962105No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG56050.16115027658620174No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC54520.15675134842961141No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC54070.15545754603061424No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC53820.15473876692006025No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT52750.15166239232688924No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA51920.14927604567985003No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG51670.14855726656929605No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC51430.14786723862316425No Hit
GGAATGAGAGTAAGCGTGAGGCTGGCGGTGCTGGCAGCAGGGTGGGCACG50950.1464871827309006No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG50200.14433084539923868No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT49130.14125447080606765No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA48660.13990316607822617No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA48530.1395294009407381No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACC48240.13869561717249548No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA47490.13653927984083356No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA46930.13492921463319263No Hit
CTTGGTCTCCTTCACATGTGTGGGCTATGCGGTCACCAAGGCCCACCCTG46640.13409543086495004No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC46570.13389417271399492No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT45070.12958149805067104No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG45050.12952399572182674No Hit
AAGCTACATTGGTTGCCCATAAATTCTTGATTGTACCAATCTTCCACAAA44700.12851770496705117No Hit
CAGCACTACCTGTGGGATCGGCTTCCAGGTGCGGCAGCGCTCCTGCAGCA44620.1282876956516739No Hit
CTCCCACATCGTACCCAGTGGCCTCGTACTCCTTGGCCTCCCTGGGCTCA44350.1275114142122756No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA44140.12690763975941027No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC44140.12690763975941027No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT44130.1268788885949881No Hit
CGCAGTGTACCATGCTGTACTCACGCCAGGGGACCACTGAGACCATCGAG43530.12515381872965856No Hit
GGCTTTGAAAAACTGGTACTACTGCTGCTGCTGCTGCTGCTACTGCTGCT42690.12273872091819718No Hit
GGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGC42010.12078364173749036No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG41540.1194323370096489No Hit
GCGGACGAACAGGGTGTAGGAGGTTTGGGTGAAGGCGGGGGCGTTGTCAT41530.11940358584522674No Hit
AGGGCCTGTCAGTCTTATTTTGGGTGTGTTCAGTGCATTAGTGGACCTCT41220.11851229974813982No Hit
GGATCACGGGTGTCTACACCGCCCTGGGCTATGTGCAGGCTTTCCTGGGA38480.11063448069646821No Hit
CCAATACACATTACTGACATGTATGCCCACCTGACCTGCACCCACTCATG38390.11037572021666878No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA38240.10994445275033639No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA37320.10729934562349774No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG36980.10632180603314435No Hit
CAGGACCCTAATAGGAGTATTCATACCAGCAGCAGCAGCAGCAGCAGCAG36500.1049417501408807No Hit
CGGAGCATGTAGGTGTAGAAGGAAGCACCGAAGAGCAGCAGCACTATGAT35640.10246915000057502No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC35370.10169286856117674No Hit
GCACACAGACCCCAACATCGACACCCATCACCACCACCACTACGGTGACC35260.10137660575253298No Hit
CCCCACGATGTCAGAAGGTGCCACAGTCTCATGGAGCATATAATACTGCA34970.10054282198429038No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)