FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001129407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048504
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC118050.38723911794112786No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC67530.22151848906873667No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT67060.2199767492514361No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA58730.19265187121289656No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC52680.1728060714370065No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC50450.16549100804853792No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA49250.16155465106819608No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA48600.15942245770384425No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC45240.14840065815888712No Hit
GGCGGCAGAATTCATCCTGTGGATGAGATGCAGGATGAGGGTGTATGAGC44810.1469901302409313No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA44630.14639967669388002No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA43760.1435458178831322No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT42060.13796931216098124No Hit
GGGCTGGACATCTTGAAGTGCCGCACCACAAAGTCCTTGCCGCCCATCAT41870.1373460556390938No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA41530.1362307544946636No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC40740.13363931948260524No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG40090.1315071261182534No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG40090.1315071261182534No Hit
CGCCATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCCC38630.12671789179217086No Hit
CACTTCTTCTGTGACTTCCCTTCCCTACTAATCCTCTCATGCAATGACAC38540.12642266501864521No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG38480.12622584716962812No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG37770.1238968359562592No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC37200.12202706639059682No Hit
CACTGGAGGAAGACAAGGATCCCACCACGAGCAGGCACGAGACAGCTCCA35290.11576169819688609No Hit
CAACTTCTACCACCAACATCACCCCCAACCACACCAGTACAGGCACCAGA35260.11566328927237754No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG35040.11494162382598153No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA35020.11487601787630917No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC33980.11146450849334624No Hit
CACAACCCCATGCTACCAGCAGTCTAGCTGCCAGCCGGATTGCTGCACCT33420.10962754190252004No Hit
GAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAGCCTTCTCTCC33240.10903708835546877No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTTGGCCTAGGC33240.10903708835546877No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA32520.10667527416726368No Hit
CACACTCGTGCAAGGCTTAGAAGGCCAGAAGATCATGTGTGTGGCTTGCG32330.10605201764537624No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT32040.10510073137512693No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG32030.10506792840029076No Hit
GGTAGCCTCATGACAGGAGGACAGGGGAGGTTGGCAGACATACGGATGAG31990.10493671650094603No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC31880.10457588377774804No Hit
GCACTGCTGCTGTTGATAAGACATCCTGCTGGAGTCTCAGGATCTGAAAG31700.10398543023069677No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT31140.10214846363987057No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC30740.1008363446464233No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT30580.10031149704904439No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)