FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001129411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939117
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC136860.4656500574832509No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT67220.2287081460180047No Hit
GGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGC63510.2160853072538453No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC62370.21220659129935962No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG59170.2013189675674701No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG57890.1969639180747143No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA57710.1963514892397955No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT52580.17889726744461007No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC50910.17321528880953022No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC50670.1723987170296385No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA50140.1705954543490443No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG48860.16624040485628847No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC45980.15644154349758788No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC44980.1530391610813724No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG42230.14368260943677982No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG40700.13847696433997012No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT39180.1333053430673226No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC38050.1294606509369991No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC36790.1251736490925676No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA36650.12469731555429743No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG36360.12371062465359493No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA36220.12323429111532477No Hit
CTCTGCTCAACCTGTCTTGGCCCGAGGTGGATGAGGGTGCTGTCCTGGGC35750.1216351713797035No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA35610.12115883784143333No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT33090.11258483415257031No Hit
GCGGACGAACAGGGTGTAGGAGGTTTGGGTGAAGGCGGGGGCGTTGTCAT32610.11095169059278688No Hit
CTTGGTCTCCTTCACATGTGTGGGCTATGCGGTCACCAAGGCCCACCCTG32330.10999902351624653No Hit
CCACCCCTAACATGCTATTGACTTGCCTTCTCCCTTCCCATCTCCACTCC32100.10921647556051699No Hit
CACGTCCGTCGAGAAGATCTGGACAAGCTCCACAGAGCTGCCTGGTGGGG31820.10826380848397664No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA31660.10771942729738218No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA31610.1075493081765714No Hit
GATGTAGGATCATGAGCAGGAGGCCCCAGGCCAGCGTGGTCGAGGTGGTC31560.10737918905576063No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT31380.10676676022084183No Hit
CCCCACGATGTCAGAAGGTGCCACAGTCTCATGGAGCATATAATACTGCA31340.10663066492419324No Hit
GGATCACGGGTGTCTACACCGCCCTGGGCTATGTGCAGGCTTTCCTGGGA30950.10530373578186918No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA30900.1051336166610584No Hit
CACTTCTTCTGTGACTTCCCTTCCCTACTAATCCTCTCATGCAATGACAC30810.10482740224359902No Hit
GGCGGCAGAATTCATCCTGTGGATGAGATGCAGGATGAGGGTGTATGAGC30720.10452118782613962No Hit
GAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAGCCTTCTCTCC30490.10373863987041004No Hit
GAGGTTTGTTGCCTCCTTGTGGGGGTGGTCCTTGTGGCTTTCCTGGAGGA30370.1033303539804642No Hit
CTGGAGGACCTGCTACGAAATAACAGAAGACCCAAGAAATGGGTGAGTGG30260.1029560919146805No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG30110.10244573455224819No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC30070.10230963925559955No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT29780.10132294835489707No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT29590.10067649569581612No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)