FastQCFastQC Report
Wed 8 May 2024
EGAF00001129451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472525
Total Bases370.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG161530.6532997644108756No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC161170.651843762954874No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA128340.519064519064519No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA112230.45390845390845386No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC98630.39890395445951005No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT91800.3712803712803713No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT88930.35967280411724856No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC79680.3222616555949889No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG76960.3112607557052002No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT75590.30571986127541684No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA72900.29484029484029484No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC72780.2943549610216277No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC69120.27955227955227957No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT66900.27057360390693724No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA57650.2331624553846776No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT57280.23166600944378724No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT54620.220907776463332No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT52610.2127784350006572No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC51570.20857220857220857No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT51510.20832954166287498No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC51390.20784420784420785No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC48980.19809708698597586No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG48770.19724775280330836No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG47190.19085752419085752No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG45880.18555929667040777No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC44120.1784410673299562No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC43730.176863732419288No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA42720.17277883944550612No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT41760.1688961688961689No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG41230.16675261119705564No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC40690.16456860901305345No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT40150.1623846068290513No Hit
TGCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCA40100.1621823844046066No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA39830.16109038331260553No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC39320.15902771458327014No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA39180.15846149179482513No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC39050.15793571349126903No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAAC38590.1560752671863783No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC38400.15530682197348863No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC36020.14568103456992346No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG35770.14466992244770022No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG35410.14321392099169877No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA34840.1409085853530298No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG34330.1388459166236944No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA34300.1387245831690276No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC33340.13484191261969042No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA32960.13330502219391108No Hit
AAGTGGTGACATCAGAACCTGCCCTCCACACCCAACAGCTGGACTGCAGC32450.1312423534645757No Hit
ACCAGCAAGATCATGGTATCTGTATGGGCCTTCTTCGCTGTCATATTCCT32020.12950324061435173No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC31470.12727879394546063No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC31450.12719790497568276No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA31280.12651034873257097No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC31090.1257419035196813No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG31080.12570145903479235No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC30820.1246499024276802No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT30800.12456901345790235No Hit
CGTGTTGTGCAGTCTCTTTCTGTGCAGGTCGTCCTTCCGGACCAGCACCA30730.12428590206367984No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT30710.12420501309390197No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC30640.12392190169967948No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC30550.12355790133567911No Hit
ACCTTACCTTTTTGTCACTGAGGCCGGTCACTTGGTCCACAAATTCCTCT30530.12347701236590125No Hit
AAAGCAGATCTCAATTAAGCAGACCAGCATTTGCACAGGATCACTCAGGC30400.12295123406234516No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC30110.12177834400056622No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG29370.11878545211878545No Hit
AACGATATTTGGCATAGCAACAGCCAGCACAAGATAACCAGCCACAGAAG29130.11781478448145116No Hit
CCTTACCTGCTCCAACCAAAGATGGGCGGGGCTGTCCACACAGCAGACCA28750.11627789405567183No Hit
GTGAACAGCATAATCGCGTATTTCGTAAAGTCTGCTCCAAAGATGGCCTC27950.11304233526455748No Hit
CTGAGGAGCAGTAAGGGATGGCATCAGCAACGCGAGCCACAGGCAGGCCT27870.11271877938544604No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT27680.11195033417255638No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT27490.11118188895966674No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA27420.11089877756544422No Hit
AAACTGACAAAGTCCATCTGGAGGCGGGGGCCGCCGTAGCGGATGAGCAT27200.11000899889788777No Hit
AACTGGAGGGACATTTGAAGGGGCCTTGAGCAGGAGGGGAGCTGGGTGCA26910.10883610883610884No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG26910.10883610883610884No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT26750.10818899707788596No Hit
CGCTCAGATGAGGATGAGACGGCGCGCGTGGCCACCTCCCGCTGTAGACC26610.10762277428944095No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGCGGGG26390.10673299562188451No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA26220.10604543937877273No Hit
GCCATCATTTCCTGGGAGAGGTGGCTGGTGGTGTGCAAGCCCTTTGGCAA26080.10547921659032769No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC25980.10507477174143841No Hit
CATCTAATTTCCCGGGCCATACCCTGCAGGTACTGGATGATGCTGAGGTT25440.10289076955743623No Hit
GACATTACCACAGGACAATTTGGCTACAGACATGTGGTCACAGTAGGTAT25430.1028503250725473No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG25320.10240543573876908No Hit
CATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCA25250.10212232434454657No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT25220.10200099088987977No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT25120.10159654604099048No Hit
CCCTTCATGGAATTAAGCATAAACTTAGATATATTGATCTACATAGTAAT25100.10151565707121263No Hit
GCTTTGTTCAGGTTTGTGTGAATACAACGCCGGATGAACTCTGTCCCTTC25030.10123254567699014No Hit
CTCTGCAGTGATCACTTCCTCCTCCACCCGGGAGTACACGGTGACTGAGC25020.1011921011921012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)