FastQCFastQC Report
Wed 8 May 2024
EGAF00001129452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472525
Total Bases370.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC171160.6922478033589144No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG158340.640397973731307No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC92040.37225103891770556No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT84170.3404212293101182No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC72820.2945167389611834No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA71620.28966340077451186No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT69230.27999716888605775No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT66040.2670953782064893No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG65160.2635362635362635No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC64120.2593300371078149No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA63560.25706514595403485No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG58210.23542734653845765No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT56890.230088674533119No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC55830.22580155913489247No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT54430.22013933125044235No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT54400.22001799779577555No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG51500.20828909717798605No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC51320.20756109644998536No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG49770.20129220129220127No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC48820.197449975227753No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT46690.18883529994641104No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC46030.1861659639437417No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT45680.1847504069726292No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA45480.1839415172748506No Hit
TGCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCA45470.18390107278996168No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC45470.18390107278996168No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC44510.18001840224062446No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC41620.16832994610772387No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA40540.1639619417397195No Hit
GCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCAT40470.16367883034549702No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA39350.15914904803793692No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA38300.15490237712459937No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC37660.15231393009170788No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC36620.14810770366325923No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG36230.14653036875259098No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC35600.14398236620458843No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA35470.14345658790103233No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAAC35360.14301169856725413No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG35280.1426881426881427No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG35280.1426881426881427No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA34960.14139391917169694No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA34400.1391290280179169No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC34380.13904813904813906No Hit
ACCAGCAAGATCATGGTATCTGTATGGGCCTTCTTCGCTGTCATATTCCT34340.13888636110858332No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT34170.13819880486547154No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG33960.137349470682804No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA33740.13645969201524757No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC32780.13257702146591036No Hit
GGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCCA32460.13128279794946462No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT32120.12990768546324102No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC31160.1260250149139038No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA30970.12525656970101415No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG30090.12169745503078837No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC30000.121333454666788No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC29710.12016056460500905No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG29620.11979656424100868No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT29270.11838100726989616No Hit
ACCTTACCTTTTTGTCACTGAGGCCGGTCACTTGGTCCACAAATTCCTCT29000.11728900617789506No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT28940.1170463392685615No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC28780.11639922751033863No Hit
GCCATCATTTCCTGGGAGAGGTGGCTGGTGGTGTGCAAGCCCTTTGGCAA28770.11635878302544969No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG28310.11449833672055895No Hit
CGTGAGCGTTTACCAATATGGCAGTGCTTTGGCTCATTTCTTCTACAGCT28270.11433655878100324No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGCGGGG28120.1137298915076693No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC27800.11243566799122354No Hit
GAAGCAAGGATGTAAAACTCAGTGATCACCAGGGCGATGAAGAGAAGACA27720.11211211211211211No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT27540.11138411138411138No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT27120.10968544301877634No Hit
AACTGGAGGGACATTTGAAGGGGCCTTGAGCAGGAGGGGAGCTGGGTGCA26860.10863388641166417No Hit
GTCGACAGACAGGATTATCGTCTCCATCTTTCTCCAAAATAAGATTGGTC26790.10835077501744168No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA26670.10786544119877454No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG26180.105883661439217No Hit
GACATTACCACAGGACAATTTGGCTACAGACATGTGGTCACAGTAGGTAT26110.1056005500449945No Hit
CGCTCAGATGAGGATGAGACGGCGCGCGTGGCCACCTCCCGCTGTAGACC25850.10454899343788233No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC25590.10349743683077017No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC25570.1034165478609923No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT25280.10224365779921336No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG25240.10208187985965764No Hit
GAGTCATCGTGGACGCATTCCTGGAAGGTGATGGTCTGGCGCCACTGGTA25080.10143476810143476No Hit
CATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCA24880.1006258784036562No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC24840.10046410046410047No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)