FastQCFastQC Report
Wed 8 May 2024
EGAF00001129453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257233
Total Bases338.5 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC195010.8639338517556673No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG174000.7708552905260555No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC133250.5903245256471086No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA122080.5408391601575911No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA121480.5381810384661221No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC88170.39061098256139265No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT83530.37005484148069784No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT79080.35034043893563493No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT68480.3033802890530131No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG64560.2860138940020813No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT64270.2847291351845379No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC62260.2758244275181162No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC59540.2637742758501227No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC54800.24277511448751637No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC53990.23918665020403299No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT50160.22221897340682153No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA49630.21987096591269045No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG48560.2151306488962371No Hit
CACAACCAGGAAGCTTATTGGACTGTCCTCAGAGATCTGCACCCTATAGA47650.21109916433084222No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT45760.2027260810027144No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG42650.18894815023526593No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT41650.18451794741615066No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT40800.18075227501990268No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC40540.1796004222869327No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC40350.17875868375130083No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA39920.17685369653908126No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA39680.1757904478624936No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA39490.17494870932686168No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG39360.1743727829603767No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT36630.16227832926419206No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT35140.1556773270637103No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC35090.15545581692275454No Hit
GCCTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC34970.15492419258446072No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC34760.1539938499924465No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC34350.15217746683660927No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC33430.1481016802430232No Hit
CCTTACCTGCTCCAACCAAAGATGGGCGGGGCTGTCCACACAGCAGCCCA32420.1436271753957168No Hit
AAGTGGTGACATCAGAACCTGCCCTCCACACCCAACAGCTGGACTGCAGC31710.14048173139414494No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT31400.13910836852021924No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT31320.13875395229469No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG30840.13662745494151468No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA29780.1319314399532525No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT29580.13104539938942944No Hit
ATGGCATTGTATGAGAGCATGAGGGAACGCAGGTTGGACAGGTGGGCAAA29110.12896320406444528No Hit
CAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACA29040.12865308986710722No Hit
CATCTAATTTCCCGGGCCATACCCTGCAGGTACTGGATGATGCTGAGGTT28900.1280328614724311No Hit
TGCTGGTCAAGGACAATGGCGAGCCTCCGCGCTCGGCCACCGCCACGCTG28670.12701391482403457No Hit
GCCATCATTTCCTGGGAGAGATGGATGGTGGTCTGCAAGCCCTTTGGCAA28570.12657089454212303No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC28330.12550764586553537No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAAC28300.12537473978096192No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT27560.12209638969481662No Hit
CTCCAAGCTATGAGTCCTTAGGGAGCACAAGATGACCACATAGGAGACCA27510.12187487955386085No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC27470.12169767144109625No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC26670.11815350918580403No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC26330.11664724022730484No Hit
TTAACACTCACAGCACTGGATGGTGGCTCTCCGCCCAGATCTGGCACTGC26270.11638142805815792No Hit
CGTGTTGTGCAGTCTCTTTCTGTGCAGGTCGTCCTTCCGGACCAGCACCA26270.11638142805815792No Hit
GCCTTGAGCAGCTGGTACGACAGCCAGGCGTTCTGGCCCGAGTCGCCGTC25910.11478655504327644No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC25710.11390051447945337No Hit
AAAGCAGATCTCAATTAAGCAGACCAGCATTTGCACAGGATCACTCAGGC25670.11372330636668879No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT25570.11328028608477725No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGCGGGG25470.11283726580286572No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC25340.11226133943638074No Hit
GGCTGTCAGGGTGAAAAATTTCTCTTCTTTGATGTGCATATTCACCTCTG25330.11221703740818958No Hit
GCTGACCTGATCTTTGCTCTCCCCCTGGCCAGTTGAGGAGGAGAACCCGG25320.11217273537999845No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC25270.11195122523904268No Hit
TGCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCA25210.11168541306989575No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTGATCATTT25080.11110948670341077No Hit
GACATTACCACAGGACAATTTGGCTACAGACATGTGGTCACAGTAGGTAT24620.10907159340661773No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC24550.10876147920927967No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA24550.10876147920927967No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA24490.10849566704013275No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG23990.10628056563057513No Hit
AGGAAGATGATGAGGTTCTTGGCGGCTGTCTGTGCAGGCTGCAGCTTCTT23790.10539452506675208No Hit
AAACTGACAAAGTCCATCTGGAGGCGGGGGCCGCCGTAGCGGATGAGCAT23790.10539452506675208No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC23620.10464139058750249No Hit
CCACCTCACTGTAGTGTCCTTCTACTATGCACCCTTTGCTTATACCTATG23620.10464139058750249No Hit
AACTGGAGGGACATTTGAAGGGGCCTTGAGCAGGAGGGGAGCTGGGTGCA23440.10384395408006174No Hit
GGCGACCAGAAGCAGTATGGCGGCTTCGATAACCGGGGCCTGGAGCCCGG23270.10309081960081215No Hit
GGTGAACTCCTGGAGGATCTCCTGGCGGAGGTGGTGGTAGAAGGTCCAGG23260.103046517572621No Hit
GTAATTGCAAAGGTTGGGACTGCCACCTTCCTGAGAGGGGTATTACTCAT22930.10158455064231296No Hit
TCTGTGTGGTCTCCTTGTTCTATGGAACAGGACTTGGGGTCTATCTGAGT22790.10096432224763682No Hit
CCCTTCATGGAATTAAGCATAAACTTAGATATATTGATCTACATAGTAAT22750.10078711413487221No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)