FastQCFastQC Report
Wed 8 May 2024
EGAF00001129461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480392
Total Bases222 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC90940.6142967538327686No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA44420.30005566093305014No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT39820.2689828099584434No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC31310.211498035655421No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC31230.21095763824716696No Hit
GGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGC26520.17914174083621096No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC25820.1744132635139882No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG25570.17272452161319435No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG25430.17177882614874979No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA24780.1673880972066858No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT24160.16320001729271705No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA23780.16063312960351042No Hit
GCTGAAATTGAACTCTGCTGTTGCTGTTGCTGTTGCTGTTGCTGCTGCTG23110.1561073013093829No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG22680.1532026652400175No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA22480.15185167171938244No Hit
TGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTG21900.1479337905095407No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT21890.14786624083350897No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT21440.14482650541208006No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG21120.14266491577906393No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTGACCT20440.13807153780890466No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC19910.1344914049792217No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC19120.12915498057271316No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA19070.12881723219255442No Hit
CGCAGTGTACCATGCTGTACTCACGCCAGGGGACCACTGAGACCATCGAG19010.12841193413636387No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT18790.1269258412636653No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT18750.1266556425595383No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG18560.12537219871493496No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC18450.12462915227858568No Hit
GAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAGCCTTCTCTCC18440.12456160260255392No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT18180.12280531102572831No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC17500.11821193305556905No Hit
GTTCTCTGTTGCATGCTTCTCCTTCTCCACCTTGGCCAGTGTCAGCTCCA17480.11807683370350555No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGATGCCTGGGGCTGGCCATT17300.116860939534934No Hit
CTCAGTGTCCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCT17160.11591524407048943No Hit
CACACTCGTGCAAGGCTTAGAAGGCCAGAAGATCACACGCGTGGCTTGTG17100.11550994601429891No Hit
GTGAAGACCAAGAACATGAAGATGATGGTGAAGATGAAGATGATGAAGAT17040.1151046479581084No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGAGGTGGCGTGACCT17030.11503709828207663No Hit
CTCCCACATCGTACCCAGTGGCCTCGTACTCCTTGGCCTCCCTGGGCTCA16950.1144967008738226No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA16800.1134834557333463No Hit
GGAATGAGAGTAAGCGTGAGGCTGGCGGTGCTGGCAGCAGGGTGGGCACG16780.11334835638128281No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG16750.11314570735318752No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG16720.11294305832509227No Hit
GTCTAAGATAATATCGCTTCTGATTCTCTTCTTCTCCATCTTCTTCATCT16600.11213246221271124No Hit
GTCACACTCTAGAGCCGAAAGGGTGCACGCAGGAGTAGTGTCATTGCACT16530.11165961448048894No Hit
CTACGTGCCCTTCTCCAATGCGACGGGTGTGGTACGCAGCCCCTTCGAGT16340.11037617063588563No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC16190.10936292549540932No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA16150.1090927267912823No Hit
CAGATGAGATCAAATTAACTCTAGTGTGCTCTGGCAGAGCCTTACCTCTC16090.10868742873509178No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA15900.10740398489048847No Hit
GAGTCGCACAGCTCAGCCTGTCCCTGACATGTCTCTGTGCTTTGTAGGCG15760.1064582894260439No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG15730.10625564039794864No Hit
CAGCACTACCTGTGGGATCGGCTTCCAGGTGCGGCAGCGCTCCTGCAGCA15650.10571524298969462No Hit
GGCACTCACCTTCTTCATCAAGGAGTTGGGATGGGAACTCACGGTGAGGT15560.10510729590540885No Hit
CTTGGTCTCCTTCACATGTGTGGGCTATGCGGTCACCAAGGCCCACCCTG15560.10510729590540885No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC15510.10476954752525007No Hit
AGCGTGAGGAAGTTGATGGGGAAGCCCAGCACGATCAGCAGAAACATGTA15400.10402650108890078No Hit
CTCTGCTCAACCTGTCTTGGCCCGAGGTGGATGAGGGTGCTGTCCTGGGC14950.10098676566747185No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC14950.10098676566747185No Hit
AGTTGAGATTGTCTGGTCTCCTGTCCGTGGTGCCGAACTGTATGAAACCA14910.10071656696334486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)