FastQCFastQC Report
Wed 8 May 2024
EGAF00001129466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186641
Total Bases477.9 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC182980.5742096458308293No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC78300.2457132761424961No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC76470.23997055206406998No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT68320.2143950322612431No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA66940.2100644534480037No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT59180.18571279287500536No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC55050.17275243744118024No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG52380.16437370886773878No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC51790.1625222295200495No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG47640.14949911207443825No Hit
GGGCTGGACATCTTGAAGTGCCGCACCACAAAGTCCTTGCCGCCCATCAT45880.14397605503726338No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA45110.14155971758349936No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA43370.13609942255811056No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT42310.1327730359334484No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC41450.13007426942664707No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC40450.12693616883734316No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTTGGCCTAGGC40400.12677926380787796No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA39700.12458259339536522No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG38630.12122482576481003No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGATGCCTGGGGCTGGCCATT37970.11915367937586946No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA37380.11730220002818013No Hit
CTCTTTTGCTTGTCCTTCTTGTTCATGTCAGTGTCCCTGAGCATGACGAT37340.117176676004608No Hit
AGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAGGACCAC37130.11651767488085416No Hit
GAGGTTTGTTGCCTCCTTGTGGGGGTGGTCCTTGTGGCTTTCCTGGAGGA36770.11538795866870474No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG36280.11385028937994585No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT36250.11375614636226672No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC35900.11265781115601037No Hit
ACAAGGAGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAG35120.1102100926963533No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC35080.11008456867278116No Hit
CTCTGCTCAACCTGTCTTGGCCCGAGGTGGATGAGGGTGCTGTCCTGGGC34490.10823308932509183No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG34230.10741718317187282No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA33820.10613056193025823No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG33610.1054715608065044No Hit
CACACTCGTGCAAGGCTTAGAAGGCCAGAAGATCACACGCGTGGCTTGTG33520.10518913175346706No Hit
GTGTGATCTTCTGGCCTTCTAAGCCTTGCACGAGTGTGGGCTTCCTGTTA33400.10481255968275058No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA33140.10399665352953157No Hit
GCTGGTTGCCTCCTTGTGGGGGTGGTCCTTGTGGCTTTCCTGGAGGAGAT33040.10368284347060117No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC32980.10349455743524294No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT32690.10258450826434481No Hit
GAGGATGAGGAGGAGATGAACGCGGAGCTCACTGCCAAGAAGCGCAAGCT32660.10249036524666569No Hit
GGCGGCAGAATTCATCCTGTGGATGAGATGCAGGATGAGGGTGTATGAGC32590.10227069820541441No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA32580.10223931719952138No Hit
GAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAGCCTTCTCTCC31870.1000112657811156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)