FastQCFastQC Report
Wed 8 May 2024
EGAF00001129550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221379
Total Bases333.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG149990.6752112088932146No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC107790.4852391239855963No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC103430.4656116763505912No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC60110.27059767828902676No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA50840.2288668435237751No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA45000.20257686779248385No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC41960.18889167494605827No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT39840.1793480536189457No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT39700.17871781447470242No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT38650.1739910208928778No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT38610.17381095256595114No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG37610.16930924439278486No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC37440.16854395400334657No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG37230.16759859528698165No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC36200.16296183586862037No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT35120.15809999104160075No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC34850.15688452983484583No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC34240.15413848784921438No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC33030.14869142095968313No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA32960.14837630138756153No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC32770.14752097683465992No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG32000.14405466154132185No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG31960.1438745932143952No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG31720.1427941832528353No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA31220.14054332916625215No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG31190.14040827792105715No Hit
CTGCAGCTTCTCTGTGGATAAGATATTCTCCATGTTTTACACAGTGATGA31050.13977803877681388No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT30750.13842752632486396No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA30600.13775227009888902No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG30480.13721206511810907No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC29500.13280039110840608No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC28590.12870383667082474No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT28490.12825366585350811No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC28290.12735332421887485No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG27920.12568769219480333No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT27820.1252375213774867No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC26950.12132103526683201No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG26670.12006055697834543No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG26350.11862001036293222No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC26290.11834990787254224No Hit
TCTGCAGCTTCTCTGTGGATAAGATATTCTCCATGTTTTACACAGTGATG25970.116909361257129No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC25820.11623410503115407No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA25680.11560386588691078No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGCATTTCTGAG25590.11519871215132582No Hit
GGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCCA25140.11317294347340098No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC25080.112902840983011No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC25060.11281280681954768No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC25030.1126777555743527No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT25030.1126777555743527No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC24610.11078703814162284No Hit
GCCATCATTTCCTGGGAGAGGTGGCTGGTGGTGTGCAAGCCCTTTGGCAA24590.11069700397815951No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA24570.11060696981469618No Hit
CCTTACCTGCTCCAACCAAAGATGGGCGGGGCTGTCCACACAGCAGCCCA23970.1079059449107964No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC23960.10786092782906474No Hit
GCAGGGAGCCTACGTTCATGGGCCTCCCCTTCCCTGGATAGATGGTACAT23940.10777089366560141No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTTC23720.10678051786750482No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT23550.10601522747806655No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA23280.10479976627131166No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC23020.10362932214628841No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC22890.10304410008377678No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC22220.10002795560775535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)