FastQCFastQC Report
Wed 8 May 2024
EGAF00001129554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316059
Total Bases347.4 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG170140.7346099559639888No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC115380.49817383754040806No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC114660.49506510844499213No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC64300.27762677893784227No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA53590.2313844336435298No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA46600.2012038553421998No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT44190.19079824823115474No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT43570.18812128706565764No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT43200.18652374572495778No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT40600.1752977795470668No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG40240.17374341499935883No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC39340.16985750363008886No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG38520.16631700660475404No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC38210.1649785260220055No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC37920.16372639902524072No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC37750.1629923935443786No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT36820.158976951796133No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG35800.1545729189109604No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC34890.15064383074869855No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT34850.15047112357673098No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG33970.1466715657934448No Hit
CTGCAGCTTCTCTGTGGATAAGATATTCTCCATGTTTTACACAGTGATGA33520.14472861010880983No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA32820.14170623459937765No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG32380.13980645570773456No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC32110.1386406822969536No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG31800.13730220171420504No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA31670.1367409034053105No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC31510.1360500747174403No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA31510.1360500747174403No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC30990.13380488148186206No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT30830.13311405279399185No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG30350.13104156673038123No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC30300.13082568276542178No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG28830.12447869419561419No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT28550.12326974399184132No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG28440.12279479926893054No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC27910.12050642924036047No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA27790.1199883077244578No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC27680.11951336300154702No Hit
CCTTACCTGCTCCAACCAAAGATGGGCGGGGCTGTCCACACAGCAGCCCA27130.11713863938699316No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC27010.1166205178710905No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA27000.11657734107809863No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG26980.11649098749211484No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGCATTTCTGAG26570.11472073897944741No Hit
GCCATCATTTCCTGGGAGAGGTGGCTGGTGGTGTGCAAGCCCTTTGGCAA26480.11433214784252042No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC26080.1126050761228449No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC25770.11126659554009635No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC25740.11113706516112068No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTTC25730.1110938883681288No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC25550.1103167060942748No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA25370.10953952382042081No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT25280.10915093268349381No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT24850.1072943305848426No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC24730.10677620906893995No Hit
GGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCCA24200.10448783904036987No Hit
GCAGGGAGCCTACGTTCATGGGCCTCCCCTTCCCTGGATAGATGGTACAT24170.1043583086613942No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC23860.10301982807864565No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC23820.10284712090667811No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC23760.10258806014872679No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG23570.10176770108188089No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC23510.10150864032392957No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA23280.10051557408511615No Hit
TCTGCAGCTTCTCTGTGGATAAGATATTCTCCATGTTTTACACAGTGATG23170.10004062936220537No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)