FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001129564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3202526
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG236080.7371680979326943No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC193400.603898297781189No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC177830.5552804255141098No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA104090.32502468364035136No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA97200.30351041646500293No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC95200.29726534616736916No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG93200.29102027586973533No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG80690.25195736115803585No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA77030.240528882513366No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA75120.23456484037912576No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG73970.23097392495798627No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT72630.22678972785857165No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT72510.2264150236407136No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT70490.22010750264010348No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC67610.21111460141151078No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT64670.20193434807398908No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG63420.19803117913796794No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT61590.19231693981563303No Hit
CCTGCTGTGGGATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCT61440.19184855954331048No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT60190.18794539060728938No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC58090.18138806679477387No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC57840.18060743300756965No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG57800.18048253160161698No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC57330.17901494008167304No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC57290.17889003867572034No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC55580.17355050357124346No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC53750.16783626424890852No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC53600.16736788397658597No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT52680.16449515163967443No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG50680.15825008134204063No Hit
CCATTCTAATAGCCTCAAAATCTCTGCACCAGGGGGATGAAAGAATGCAT49600.15487774338131838No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC48980.1529417715890519No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC48560.1516303068265488No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA48120.15025639136106936No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG47970.14978801108874684No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT47750.1491010533560071No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC47170.1472899829696933No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA46580.14544768723189133No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG45420.1418255464592637No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC43330.1352994479982364No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG43330.1352994479982364No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA42840.13376940577531612No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC42170.13167730722560878No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG40610.1268061523934544No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG40340.12596306790327386No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC39720.12402709611100739No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT39560.12352749048719666No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA38910.12149784264046569No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC38900.12146661728897751No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC38750.12099823701665498No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT38270.11949942014522286No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC37780.11796937792230258No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG37280.11640811034789413No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC37190.11612708218450062No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC36850.11506542023390287No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG36350.11350415265949441No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTTC36160.11291087098121921No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT34350.1072590823618606No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA34140.10660334998060905No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG33620.10497963170322426No Hit
GCAGCATGATGAGCAGGTTCCCCAGCACCGTGGTCAGGTACATGCCCAGG33540.1047298288913189No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC33460.10448002607941356No Hit
CTGGAGAACAATGACCAGGTATTGGGAGGGCTGGTCTCAACACATTTCAC32700.10210689936631272No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG32480.10141994163357299No Hit
AAAAAGTCCTCCAGATCTCGAGGCCGAATTCGGGCAGCTAACTGCATACA32400.10117013882166766No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC32350.10101401206422679No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA32340.10098278671273864No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC32280.10079543460380962No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG32260.10073298390083328No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)