FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161546
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG270991.253686019173314No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC217241.0050214059751679No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC115000.5320266142844057No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA97030.448891672904486No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG84220.38962853439158823No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA82540.3818563195046509No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG81680.3778776856934805No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA76730.3549774096873256No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA73320.3392016639941967No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA71900.3326322918873806No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC69760.3227319705433056No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC65830.30455053928993414No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT62930.291134215973197No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT60730.280956315526017No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT60370.27929084090738754No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC59310.2743869434192009No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC58370.270038204137224No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA56980.2636076215819603No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG55180.2552802484888131No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT52560.24315929431989883No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG51320.23742265952239738No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC49850.23062197149632718No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC48280.22335865163174876No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG47040.2176220168342473No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC44540.20605622087154288No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT43670.2020313238765217No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG42910.19851532190385954No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC41770.19324131894486632No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG40980.18958652742065168No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC39780.18403494535855355No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG39490.18269331302687983No Hit
CCATTCTAATAGCCTCAAAATCTCTGCACCAGGGGGATGAAAGAATGCAT38470.1779744682740964No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA38330.17732678370018495No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT38300.17718799414863248No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC38290.1771417309647817No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC38030.1759388881846604No Hit
CCTGCTGTGGGATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCT36310.16798162056231974No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC35700.16515956634741985No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC35700.16515956634741985No Hit
GTGAACAGCATAATCGCGTATTTCGTAAAGTCTGCTCCAAAGATGGCCTC35570.16455814495735924No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT35550.16446561858965758No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG34260.15849766787290206No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC33740.15609198231265956No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG33640.15562935047415138No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG33070.15299234899465475No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC32970.15252971715614658No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT32800.15174324303068265No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT32610.15086424253751712No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC32460.15017029477975485No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG31860.1473945037487058No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA31760.1469318719101976No Hit
CTGAGGAGCAGTAAGGGATGGCATCAGCAACGCGAGCCACAGGCAGGCCT31510.14577529231392716No Hit
CACAATGTTAACAAAACTCCCTATTTTCCCCACCCCATAGTAGCTCAGCA30910.14299950128287808No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA30540.14128776348039784No Hit
GTCTCACACCCATCTCTCCTCTGCCCTCCAGCTCCCCCGCCTCCAAAGCG30100.13925218339096182No Hit
CAGGATTCCCTCTACCTACCTTCCCCCCATTTAGGCGTGCATTAGGCCTC29740.1375867087723324No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC29660.13721660330152585No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC29050.13439454908662596No Hit
CTGGAGAACAATGACCAGGTATTGGGAGGGCTGGTCTCAACACATTTCAC28850.13346928540960962No Hit
CCGGCATGGTGACTGTACAGGGCTGGGCCGGGTGTGCGACTTACCGAGCT28830.133376759041908No Hit
GATATAAACATGAGCCCTGAGAACCAGAGCAGCGTGTCCGAGTTCCTCCT28510.1318963371586818No Hit
GGCCTCCAGTATGACTGTGGCCTCCAGTGCTCCCACGACTGCAGCCTCCA28500.13185007397483098No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG28370.13124865258477034No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT28060.12981449388539498No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT27990.12949065159843925No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT27650.12791770334751146No Hit
TACTCACGGAGGAGATTCAGGGTGACTGGGTCACTGCGGCTGGCACTCAC27510.1272700187736No Hit
GCAGCATGATGAGCAGGTTCCCCAGCACCGTGGTCAGGTACATGCCCAGG27270.12615970236118038No Hit
CACAATGTTAACAAAACTCCCTATTTTCCGCACCCCATAGTAGCTCAGCA27050.12514191231646238No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG26940.12463301729410338No Hit
GGGAGTGCAAGTGTCATGGGAGTGCTGGAGGCCGCAGTCATACTGGAGGC26830.12412412227174438No Hit
GCAACTTGCATACCAGTGTTTCCTCAGCACAGGGCCAGGATGCAGCCCAG26450.12236612128541333No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA26410.12218106855001003No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC26110.12079317303448552No Hit
AGAAGGAGACTATGCAGTTCCTGAACGACCGCCTGGCCAGCTACCTGGAG26100.12074690985063469No Hit
GAGGTAGAGAAGCTTGGCATCCCAGCCAGCTGAGTAGCGAGTACGGGGGT26070.12060812029908224No Hit
AAAAAGTCCTCCAGATCTCGAGGCCGAATTCGGGCAGCTAACTGCATACA25660.11871132976119869No Hit
GGAGAACCAGAAAATGATGAACCAAGATGCCAGAACCAGGAGGCCACAGA25470.11783232926803316No Hit
ATACAACACTAATGACCCTCCACGTTGGTTTGGCTATGATAGGGAAGGAA25340.11723090787797252No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT24990.1156116964431939No Hit
GTCTTAAAATAGGACTGGTAACTGGGGCACAGCAAGGGCTCCTGCTGCTG24490.11329853725065299No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGCCTTTCTAGACTAAATTGTT24320.1125120631251891No Hit
GGGTCCTCCTGGATGCTAATCTGGATCAGGCTCCGGCCACGGATTGTACA24200.11195690491897928No Hit
ATCATCCTGGTGTTTCCACTCCTGGCCACCATGCTGTCATATGCGCACAT24110.11154053626432194No Hit
CTTGGTCTTCACTGCTGTATGTTGACTTTTTCACTGTTAATTCTAAGGCT24100.1114942730804711No Hit
CCAGGTCTATATGAGAAACCTTCTCTCTCAGCCCAGCCGGGCCCCACGGT23500.10871848204942203No Hit
GGTCTGTAGGAGATGGTATTTTGGGGGCAGCTGCAAGGGAAAAAACTGAT23470.10857969249786958No Hit
GGTGGAGGCCATAGGAATGGGAACATCATGGGCCACATCCCCTTGCCCTT23390.10820958702706304No Hit
CCATCACCTTCGAGCAGTTCCTGGCCTCCCTCTTAACTGAGCCTGCCCTG23350.10802453429165977No Hit
CATAAGCATAGCACCTGTACCACCACCTGCGACTCGGGCTCACGGGGCCC23310.10783948155625649No Hit
CGCAGTCATTGATCCACATGATCTCCCTGGACGTGGCCTGAATGATGTTC23190.10728432335004667No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC22830.10561884873141723No Hit
AGTCATCACCAAAGAGCAAGAGGGTGACCCTCCCACGGCTCCCACCCTGG22770.10534126962831233No Hit
CTGACTTTTATTTGCAGCCACTTTGGCAAACAGGGCTTGAGACACCTTGG22600.10455479550284842No Hit
GACAACAGACCTCCCTATTAAGGTGGACGGCGCCAACATCAACATCACAG22430.10376832137738451No Hit
GACAAGGATTGGGATTATGGAAAATGGAGGAGCAGCAGCAGTCATAAAAG22390.10358326864198125No Hit
GGTTGTCTACCTGAGCTACATGGCGGTGGAGTACAATGTGTCCTTCCCCC22240.10288932088421898No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAAC22190.1026580049649649No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA22160.10251921541341244No Hit
CGGATAAAATGCACCCAACTGCATCCACCGCCTACAGAGCTCCTCAGGGG22100.10224163631030753No Hit
AGAGCAGGGAAGACTAGAGGCCAGGAGAGGATAGGCAACCTGAGGAGACG21830.10099253034633546No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT21810.10090000397863381No Hit
TGTGATCTTAACCTGTGATCCTGAGACTCCGGACGCAAGCTACCTGTGGT21690.10034484577242399No Hit
AAAGCAGATCTCAATTAAGCAGACCAGCATTTGCACAGGATCACTCAGGC21640.10011352985316992No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)