FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161546
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG267121.2357821670230473No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC196790.9104131950002451No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC124430.575652796655727No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG84940.39295948362884714No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG79650.36848625937176444No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA78540.36335104596432366No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA74320.34382798237927853No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT66600.3081128044464471No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC65650.30371780198061943No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG64970.3005719054787638No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC56170.2598603036900441No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA55460.256575617636636No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT55250.25560409077576884No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT54170.25060766691988046No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT53480.24741550723417408No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC51390.2377465018093531No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG50710.2346006053074975No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG50550.2338603943658844No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC50290.23265755158576312No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT46070.213134488000718No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC43830.2027715348181348No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC43800.20263274526658234No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA43080.19930179602932344No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA42680.19745126867529075No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC40620.18792105280202226No Hit
CCTGCTGTGGGATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCT40100.18551536724177972No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG39740.18384989262315027No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA39660.18347978715234373No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC39530.1828783657622831No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG39520.1828321025784323No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG39510.18278583939458146No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA39140.18107410159210122No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG38630.1787146792157095No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT37700.17441220311758343No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC35960.1663624091275411No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC35770.16548340863437558No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC35660.1649745136120166No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC35390.16372540764804452No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC35090.16233751213251996No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC34780.1609033534331446No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG34200.15822008876979718No Hit
CCATTCTAATAGCCTCAAAATCTCTGCACCAGGGGGATGAAAGAATGCAT33610.1554905609225989No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG32330.14956887338969424No Hit
GCAGCATGATGAGCAGGTTCCCCAGCACCGTGGTCAGGTACATGCCCAGG32310.1494763470219926No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT32130.14864360971267787No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA31700.14665429280709272No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG31100.14387850177604364No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC30100.13925218339096182No Hit
CCGGCATGGTGACTGTACAGGGCTGGGCCGGGTGTGCGACTTACCGAGCT29650.13717034011767504No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA29490.13643012917606195No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC28720.13286786401954898No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC28690.1327290744679965No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT28470.13171128442327854No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT28240.13064723119470972No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG28130.1301383361723507No Hit
GTCTCACACCCATCTCTCCTCTGCCCTCCAGCTCCCCCGCCTCCAAAGCG27770.12847286155372128No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT27720.12824154563446719No Hit
GGGAGTGCAAGTGTCATGGGAGTGCTGGAGGCCGCAGTCATACTGGAGGC27630.12782517697980983No Hit
GGAGAACCAGAAAATGATGAACCAAGATGCCAGAACCAGGAGGCCACAGA27330.1264372814642853No Hit
CTGGAGAACAATGACCAGGTATTGGGAGGGCTGGTCTCAACACATTTCAC26960.12472554366180502No Hit
GGCCTCCAGTATGACTGTGGCCTCCAGTGCTCCCACGACTGCAGCCTCCA26380.12204227899845758No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC26380.12204227899845758No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA26150.12097822576988879No Hit
GCAACTTGCATACCAGTGTTTCCTCAGCACAGGGCCAGGATGCAGCCCAG25860.11963659343821506No Hit
CAGGATTCCCTCTACCTACCTTCCCCCCATTTAGGCGTGCATTAGGCCTC25810.11940527751896096No Hit
GGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGCC25650.11866506657734786No Hit
AAAAAGTCCTCCAGATCTCGAGGCCGAATTCGGGCAGCTAACTGCATACA25500.11797111881958562No Hit
GTCTTAAAATAGGACTGGTAACTGGGGCACAGCAAGGGCTCCTGCTGCTG25470.11783232926803316No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC24950.11542664370779064No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG24790.11468643276617754No Hit
CTTGGTCTTCACTGCTGTATGTTGACTTTTTCACTGTTAATTCTAAGGCT24530.11348358998605629No Hit
AGAAGGAGACTATGCAGTTCCTGAACGACCGCCTGGCCAGCTACCTGGAG24470.11320601088295136No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC24200.11195690491897928No Hit
GACAACAGACCTCCCTATTAAGGTGGACGGCGCCAACATCAACATCACAG23960.11084658850655967No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG23950.11080032532270884No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC23740.10982879846184167No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGCCTTTCTAGACTAAATTGTT23720.10973627209414002No Hit
GCCGAGATTTCATAGTCACTGGCCGAGGTGAGGGGCTTGCCCATCATCCG23710.1096900089102892No Hit
ATACAACACTAATGACCCTCCACGTTGGTTTGGCTATGATAGGGAAGGAA23230.10746937608544994No Hit
GATATAAACATGAGCCCTGAGAACCAGAGCAGCGTGTCCGAGTTCCTCCT22990.10635905967303033No Hit
GAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCGC22970.10626653330532869No Hit
GAAGGGTCAGATCCTATCCACCTCCCTGGCCCCAGCTTAGTCCTTTCAGG22530.1042309532158927No Hit
GCCAATGAGTTCGGCAACTCCTGGGAGGAGGTGGTGCCCGACTCTCCCTG22500.10409216366434025No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC22310.10321316317117471No Hit
GAAGCAAGGATGTAAAACTCAGTGATCACCAGGGCGATGAAGAGAAGACA22200.10270426814881571No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT22110.10228789949415834No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT22030.1019177940233518No Hit
GAAGCGGAGGCCTACATGGGGGCAGGGAGAAGGCCAGGAAGGGGGAAAGC21830.10099253034633546No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCACGG21750.10062242487552889No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)