FastQCFastQC Report
Wed 8 May 2024
EGAF00001129574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388455
Total Bases358.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG107360.4494955944323841No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC66400.2780039816534119No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC65260.2732310217274347No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC63840.2672857558547262No Hit
CAGTAAATTACATTTAAGAAATTCTGAGCATTATGGGGGTGTATAGATCG62160.2602519201743386No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC60180.2519620424081676No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA59760.2502035834880707No Hit
ATACACCCCCATAATGCTCAGAATTTCTTAAATGTAATTTACTGAGATCG55160.23094427150605726No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT54410.22780416629159853No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG52140.21830011450917017No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAAC47760.19996190005673126No Hit
CACTTAATTTTGTTTCTGATCTCACCATCCAAGCCATTCAGATCGGAAGA47260.19786849658042543No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA44290.18543367993116888No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC43340.18145621332618786No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG42910.17965588633656485No Hit
CAAACAGAAAATGGAATTTCACATGCCCAACCCAGAGATCGGAAGAGCGT40110.1679328268692523No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC40050.16768161845209562No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT39910.16709546547872997No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC38680.16194569292701766No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA38180.15985228945071187No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT37600.1574239414181971No Hit
GAAGGCTGTGTACATGACCTCCTTGGACACGGAGATCGGAAGAGCGTCGT37390.15654471195814867No Hit
GAAGTAGATGATGGACAGGATCACAGGTTAAGATCACAAGATCGGAAGAG36740.15382328743895113No Hit
TACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAACA36250.15177175203217144No Hit
GATGGGAAGAGCTGGAACACAAGCTGGATTCTAGATCGGAAGAGCGTCGT34010.14239330445832138No Hit
GTGATCTTAACCTGTGATCCTGTCCATCATCTACTTCAGATCGGAAGAGC33900.1419327556935341No Hit
TGTGATCTTAACCTGTGATCCTGTCCATCATCTACTTCAGATCGGAAGAG33840.14168154727637738No Hit
CCGTGTCCAAGGAGGTCATGTACACAGCCTTCAGATCGGAAGAGCGTCGT33590.1406348455382245No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC33540.14042550519059394No Hit
AGAATCCAGCTTGTGTTCCAGCTCTTCCCATCAGATCGGAAGAGCGTCGT33120.13866704627049703No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT32950.13795528908855306No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT32850.1375366083932919No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG32740.13707605962850464No Hit
CCGATACAATAAAGAACAAGAACCCATGACCCTAAACAGATCGGAAGAGC32490.13602935789035173No Hit
GTCCGGCGATAATAAGCAAACTAGAAGTATAAGGTGACAAGATCGGAAGA31780.13305672495399745No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA31680.1326380442587363No Hit
GATATCAGAGTCTCACTGGTCTCCATCAACGCAGAGATCGGAAGAGCGTC31430.13159134252058338No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC31410.13150760638153117No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC31390.13142387024247892No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA31160.13046090464337826No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA30260.12669277838602777No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC30120.12610662541266215No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC30070.12589728506503156No Hit
CTGCGTTGATGGAGACCAGTGAGACTCTGATATCAGATCGGAAGAGCGTC30010.1256460766478749No Hit
TGTCACCTTATACTTCTAGTTTGCTTATTATCGCCGGACAGATCGGAAGA29920.12526926402213984No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA29570.12380388158872577No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG29490.12346893703251682No Hit
TACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAACA29160.122087290738155No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT28910.12104058900000209No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT28590.11970081077516638No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG28180.11798421992459561No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA28110.11769114343791279No Hit
TCAAACAGAAAATGGAATTTCACATGCCCAACCCAGAGATCGGAAGAGCG27940.1169793862559688No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG27710.11601642065686814No Hit
GTCTTCCAGTGTGATGATGGTTCCTGCAGGCAGATCGGAAGAGCGTCGTG27010.11308565579004001No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC26590.11132719686994313No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC26570.11124346073089089No Hit
CTGGGTTGGGCATGTGAAATTCCATTTTCTGTTTGAAGATCGGAAGAGCG26530.11107598845278642No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC26410.11057357161847303No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC26350.11032236320131633No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG25680.10751720254306654No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC25320.10600995204012637No Hit
GTTTAGGGTCATGGGTTCTTGTTCTTTATTGTATCGGAAGATCGGAAGAG25090.10504698644102567No Hit
GGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGCC24980.1045864376762384No Hit
CGCAGTCATTGATCCACATGATCTCCCTGGACGTGGCCTGAATGATGTTC24910.1042933611895556No Hit
TCCGATACAATAAAGAACAAGAACCCATGACCCTAAACAGATCGGAAGAG24820.10391654856382056No Hit
GTTGTGGAAATATTGCTTCGATCAGGGCATTCATGATAGATCGGAAGAGC24790.1037909443552422No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA24700.10341413172950714No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA24640.10316292331235045No Hit
GCCTGCAGGAACCATCATCACACTGGAAGACAGATCGGAAGAGCGTCGTG24500.10257677033898482No Hit
CTTCAGTTGAAGTGGTCACAGACACAGAGTTAGGAGATCGGAAGAGCGTC24330.10186501315704086No Hit
GTGTGTCCTGTGTTGTTCATAGTCCTCTGTGTATCTAGATCGGAAGAGCG24300.1017394089484625No Hit
GTCACCTTATACTTCTAGTTTGCTTATTATCGCCGGACAGATCGGAAGAG24110.1009439156274663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)