FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393710
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC205250.8574555815031896No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG169130.7065601096206308No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC101350.42340133098829846No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA98730.4124559783766622No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT89380.37339527344582263No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA87370.3649982662895672No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA81310.33968191635578243No Hit
GTCTCAAATCAATGCCTAAGTCCCACCACCCCTTCCAGGTCCCACGCTGC72690.3036708707403988No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT72220.3017073914551052No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC65270.2726729637257646No Hit
CAGTGTCTACCGCTTGGAACGGGGTGTAGGAGGCGCACCCCCCACTGTGA65250.2725894114157521No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT60760.2538319178179479No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT59320.2478161514970485No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT57480.24012933897589933No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT56420.2357010665452373No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA56410.23565929039023104No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC52190.21802975297759544No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC49340.20612354880081546No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC48310.20182060483517217No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG47800.19969002092985366No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC46390.19379958307397305No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT44800.18715717442798No Hit
GCTCCAAATTTCATATTCGTCGAAAGCGATATGCATTGACAGGACAGAAA42290.17667135952141236No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC42080.17579406026628122No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA41800.17462432792610635No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA41350.1727444009508253No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC40560.1694440847053319No Hit
GCAGAAGATACTAAACTATGTGTCAAAACAAATAGTAATGAAATATGGGG39920.16677041078493218No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG38340.16016977829394538No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG36780.15365269811297108No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG36770.15361092195796483No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG36660.15315138425289612No Hit
GCCTGTTTCTCCATCTGAACTGCGCTCCTGGCCGCCCATGGCCTCCCAGT36050.15060303879751516No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC35480.14822179796215917No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT35430.14801291718712792No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA34810.1454227955767407No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG34770.14525569095671573No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG34620.14462904863162204No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC34070.14233136010627853No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC34030.14216425548625355No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC33510.1399918954259288No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT32610.1362320414753667No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA32550.1359813845453292No Hit
CGACCTTATCCAGCCACACGCCACCATGTGCCACACCAGCTGCTCCTCGG32520.13585605608031048No Hit
CTGAGGAGCAGTAAGGGATGGCATCAGCAACGCGAGCCACAGGCAGGCCT32340.13510408529019804No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA32170.13439389065509189No Hit
GCCAATGAGTTCGGCAACTCCTGGGAGGAGGTGGTGCCCGACTCTCCCTG31080.12984028975941111No Hit
TGCCCTCATGATTCTCCTGATTCACTGGGACCACCGGCTCCACACGCCCA31030.1296314089843799No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC30810.1287123335742425No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA30650.12804391509414256No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG30490.12737549661404263No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC30130.12587155503381778No Hit
ATGGCATTGTATGAGAGCATGAGGGAACGCAGGTTGGACAGGTGGGCAAA29970.12520313655371787No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA29920.12499425577868664No Hit
AAGTGGTGACATCAGAACCTGCCCTCCACACCCAACAGCTGGACTGCAGC29490.12319788111341808No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG29130.12169393953319324No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA28780.12023177410797464No Hit
CAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACA28750.1201064456429559No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC28570.11935447485284349No Hit
GGTTGTCTACCTGAGCTACATGGCGGTGGAGTACAATGTGTCCTTCCCCC28160.11764165249758742No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC27810.11617948707236883No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC27750.11592883014233137No Hit
ACCAGTGGTTCGAGAGACAGGTGAGGTGGTCGACTGCCACCTCAGTGACA27550.11509330704220645No Hit
GCCCTTCGTGTGACTAGAGCCTGCGGTCCCACAGATCACGCTGACCACCA27450.11467554549214398No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC27450.11467554549214398No Hit
CCTCGGGAGCCTACAAGACCTCGTCCCCCTCCAAGGCAGGCAGGGGCAGC27360.11429956009708778No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT27200.11363114161698785No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG26990.1127538423618567No Hit
GCTTTGTATATGAGGCCTGTGGGGCAAGAACCTTTGTGCAGCGTTTCCGC26880.112294304656788No Hit
CACAATGTTAACAAAACTCCCTATTTTCCCCACCGCATAGTAGCTCAGCA26810.11200187157174428No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC26770.1118347669517193No Hit
GGAATTGGCATTGGAGAAGAGGCTGGCCCAGGGAGTGAGGATGGCACGTG26670.11141700540165683No Hit
CAGGATTCCCTCTACCTACCTTCCCCCCATTTAGGCGTGCATTAGGCCTC26430.11041437768150694No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC26360.11012194459646323No Hit
CATCTAATTTCCCGGGCCATACCCTGCAGGTACTGGATGATGCTGAGGTT26360.11012194459646323No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT26210.10949530227136955No Hit
GGTGGAGGCCATAGGAATGGGAACATCATGGGCCACATCCCCTTGCCCTT26210.10949530227136955No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT26190.10941174996135705No Hit
CAGAGGACTGGCATCTCACGGGCACACACACGGCTGGCTTGAAGCTCACG25900.10820024146617595No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG25670.10723938990103228No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTATACCTCACTGTGGTGTC25390.10606965756085741No Hit
GGAGAACCAGAAAATGATGAACCAAGATGCCAGAACCAGGAGGCCACAGA25380.10602788140585118No Hit
CACAATGTTAACAAAACTCCCTATTTTCCCCACCCCATAGTAGCTCAGCA25290.10565189601079496No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG25140.10502525368570129No Hit
CCTTCTTATCCAGGCCTTCAGCATGCTGGCCTCCTTGAATCTCCTTCGGC24710.10322887902043272No Hit
AGACCAAGAAAATGTCCTTCCAAAAAATTCTCCCTGACCGGATGAAGGCT24660.10301999824540149No Hit
GCAACTTGCATACCAGTGTTTCCTCAGCACAGGGCCAGGATGCAGCCCAG24560.10260223669533904No Hit
CCTTACCTGCTCCAACCAAAGATGGGCGGGGCTGTCCACACAGCAGCCCA24550.10256046054033278No Hit
AGGAGCTCACTCACTTTAATAGCAGTGCTTTCATCAAAGGACTTTGGTGC24250.10130717589014542No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG24110.10072230972005798No Hit
GTGCTCCTTCATTTTTGATCTCCCCACTAGGTGCAATACCCTGATTATCA24010.10030454816999552No Hit
CCTGCTCTGAGTCATCCTCTTCATGCTGCCAGCAGTCTAGCTGCCAGCCG23950.10005389123995806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)