FastQCFastQC Report
Wed 8 May 2024
EGAF00001129698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891799
Total Bases283.7 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG159800.844698617559265No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC107640.5689822227414223No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC87140.46061975928732385No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC52740.2787822596375196No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA41400.2188393164390086No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG37110.19616248872105332No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT36910.19510529395564752No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG36030.19045363698786183No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA35730.18886784483975308No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA35250.18633057740277903No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC34690.1833704320596427No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT34170.1806217256695875No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT33040.17464857524504454No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC32800.17337994152655753No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG31720.167671089793366No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT30920.16344231073174265No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC30090.1590549524553084No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG29840.1577334589985511No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG29120.1539275578430901No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT28300.14959305930492614No Hit
CTGCAGCTTCTCTGTGGATAAGATATTCTCCATGTTTTACACAGTGATGA28130.1486944437543312No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT27880.14737295029757388No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA27700.14642147500870864No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC27680.14631575553216808No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC27010.1427741530680585No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC26920.14229841542362587No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA26270.1388625324360569No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC26120.1380696363620025No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC25370.13410515599173062No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC25270.1335765586090277No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG24700.13056355352762106No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG24580.12992923666837755No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC24290.1283963042585391No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA24210.12797342635237674No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA23430.123850366767294No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC23420.1237975070290237No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG23100.12210599540437436No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG22860.12083736168588735No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA22860.12083736168588735No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC22640.11967444744394093No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG22420.1185115332019945No Hit
TCTGCAGCTTCTCTGTGGATAAGATATTCTCCATGTTTTACACAGTGATG22290.11782435660448069No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC22230.11750719817485895No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC21990.11623856445637196No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT21960.11607998524156106No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC21750.11496993073788495No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT21740.11491707099961464No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC21720.11481135152307408No Hit
CATACACCCTCATCCTGCATCTCATCCACAGGATGAATTCTGCCGCTGGC20880.11037113350836955No Hit
CAGGATTCCCTCTACCTACCTTCCCCCCATTTAGGCGTGCATTAGGCCTC20880.11037113350836955No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT20780.10984253612566662No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC20770.10978967638739634No Hit
GAAGGGTCAGATCCTATCCACCTCCCTGGCCCCAGCTTAGTCCTTTCAGG20610.10894392057507166No Hit
CCTTACCTGCTCCAACCAAAGATGGGCGGGGCTGTCCACACAGCAGCCCA20600.10889106083680138No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT20440.1080453050244767No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG20440.1080453050244767No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT20260.10709382973561146No Hit
AGAGCAGGGAAGACTAGAGGCCAGGAGACGACAGGCAACCTGAGAAGACG20220.10688239078253028No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG20140.10645951287636794No Hit
GGAGAACCAGAAAATGATGAACCAAGATGCCAGAACCAGGAGGCCACAGA20070.10608949470847591No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA19930.10534945837269183No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGCATTTCTGAG19880.10508515968134036No Hit
GCAGGGAGCCTACGTTCATGGGCCTCCCCTTCCCTGGATAGATGGTACAT19690.10408082465420482No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT19620.10371080648631276No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG19490.10302362988879898No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC19290.10196643512339312No Hit
GCAACTTGCATACCAGTGTTTCCTCAGCACAGGGCCAGGATGCAGCCCAG19270.10186071564685256No Hit
GCCATCATTTCCTGGGAGAGGTGGCTGGTGGTGTGCAAGCCCTTTGGCAA19180.10138497800241991No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTTC18950.10016920402220321No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)