FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369614
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG238391.0060288300119766No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC181070.7641328925301758No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC171800.7250125969883703No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT83970.35436151204373373No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT69540.2934655180126383No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA67450.2846455161051547No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT62580.2640936456317358No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA62230.26261661181947776No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC59130.24953431233947806No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA58670.2475930679005104No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT58260.24586282829186526No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA57660.24333077032799433No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT54420.22965765732309143No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA53800.22704119742709153No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC52780.22273669888851094No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC49610.20935899264605964No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC49170.20750215013922096No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG48970.2066581308179307No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC44810.18910252893509238No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT43270.18260358016115705No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG43140.18205496760231835No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG42800.18062013475612484No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA37670.15897103916502855No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA37540.15842242660618985No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG37150.15677658892967378No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC37020.15622797637083508No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC36110.1523876884589642No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG35310.149011611173803No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT34230.14445390683883536No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC34190.14428510297457728No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT33830.14276586819625475No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT33700.14221725563741605No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG33090.13964299670748062No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC32490.13711093874360972No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG31940.1347898856100614No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC31680.13369266049238399No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC31660.13360825856025496No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT31460.13276423923896466No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC31290.1320468228158679No Hit
GGAGAACCAGAAAATGATGAACCAAGATGCCAGAACCAGGAGGCCACAGA31200.13166701412128726No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC31150.1314560092909647No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG30650.12934596098773893No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC30440.12845974070038413No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC30440.12845974070038413No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC30330.12799553007367445No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA30190.12740471654877125No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG29930.12630749143109385No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG29920.12626529046502932No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT29910.12622308949896482No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC29560.12474605568670678No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC29420.12415524216180358No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG29110.12284701221380359No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA28800.12153878226580364No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC28680.12103237067302944No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT28530.12039935618206171No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA28280.11934433203044884No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG28220.11909112623406176No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG28140.11875351850554562No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG27670.11677007310051343No Hit
GCAGCATGATGAGCAGGTTCCCCAGCACCGTGGTCAGGTACATGCCCAGG27510.11609485764348118No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC26660.11250777552799739No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC26520.11191696200309417No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG25710.10849868375186844No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC25640.10820327698941686No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC25400.10719045380386848No Hit
CCTGCTGTGGGATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCT25270.10664184124502979No Hit
GAAGGGTCAGATCCTATCCACCTCCCTGGCCCCAGCTTAGTCCTTTCAGG25130.10605102772012656No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA24680.10415198424722338No Hit
TGCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCA24640.10398318038296532No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG24550.1036033716883847No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC24440.10313916106167503No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC24150.10191533304580408No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG24090.10166212724941699No Hit
GGTTGTCTACCTGAGCTACATGGCGGTGGAGTACAATGTGTCCTTCCCCC23930.10098691179238475No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT23860.10069150502993315No Hit
CATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCA23850.10064930406386863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)