FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249945
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA171480.7621519637146685No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA167460.744284860296585No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA151710.6742831491436457No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA138080.6137038905395465No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC134370.5972145985790764No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG126570.5625470844842874No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC117020.5201016024836163No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC111540.49574545155548244No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC97570.4336550449010976No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT97020.431210540702106No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG94720.4209880685972324No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC78730.34991966470291497No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC77070.3425417065750496No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC76960.34205280573525126No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT66330.2948072063983786No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC56770.25231727886681676No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC55950.24867274533377481No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC55080.24480598414627913No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT54020.24009475787185908No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT53890.23951696597027927No Hit
CCTGCTGTGGGATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCT53820.23920584725404398No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT53590.2381836000435566No Hit
CCATTCTAATAGCCTCAAAATCTCTGCACCAGGGGGATGAAAGAATGCAT53120.23609466009169114No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT52050.23133898828638033No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC51660.2296056125816409No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT49700.2208942885270529No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC45990.20440499656658273No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG43370.1927602674732049No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT43290.19240470322607886No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA41210.1831600328008018No Hit
GGTTAGGTAGGAGCTTACTCAGTGCATCTGGAGGATGGAAAACCAATGGC39360.1749376095860121No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTACTT39050.1735597981283987No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT36880.16391511792510485No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG36620.16275953412194522No Hit
AAAGCAGATCTCAATTAAGCAGACCAGCATTTGCACAGGATCACTCAGGC35190.15640382320456722No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC35080.15591492236476892No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG35020.15564824917942438No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG34570.1536482002893404No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA34520.15342597263488664No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA34440.1530704083877606No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT33890.15062590418876906No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC32800.14578134132167675No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG32750.14555911366722296No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC32420.14409241114782806No Hit
CACAACCAGGAAGCTTATTGGACTGTCCTCAGAGATCTGCACCCTATAGA32270.14342572818446675No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA32190.14307016393734068No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC32010.14227014438130708No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG31040.13795892788490385No Hit
AAGTGGTGACATCAGAACCTGCCCTCCACACCCAACAGCTGGACTGCAGC30770.13675889855085346No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA30600.13600332452571062No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC30340.13484774072255098No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG30140.1339588301047359No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC30120.13386993904295438No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC30070.1336477113885006No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC30060.13360326585760987No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGCATTTCTGAG29950.13311436501781154No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG29860.13271435523979475No Hit
CAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACA29690.13195878121465193No Hit
CATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCA28720.12764756471824867No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT28670.12742533706379489No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT28620.1272031094093411No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC28490.1266253175077613No Hit
GGTGGCGTGACCTGTAGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGG28430.12635864432241678No Hit
ACCAGCAAGATCATGGTATCTGTATGGGCCTTCTTCGCTGTCATATTCCT28370.12609197113707224No Hit
CGAGTGCGATGACCAGAGCGACGAGGAGGGCTGCCCCGTGTGCTCCGCCG28270.1256475158281647No Hit
ACCTTACCTTTTTGTCACTGAGGCCGGTCACTTGGTCCACAAATTCCTCT28250.1255586247663832No Hit
CGTGTTGTGCAGTCTCTTTCTGTGCAGGTCGTCCTTCCGGACCAGCACCA28210.12538084264282015No Hit
GCCCCAAGCTCGTATCCCGGAGGGCAGGGCCAGGGTCTTGGCAGAGCCTT28200.1253363971119294No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG27980.1243585954323328No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC27980.1243585954323328No Hit
CCTGGCCTCCTCTTGATGGCACAGGACCTTCCCCATCCCGCCCCTGGAGC27800.1235585758762992No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTGATCATTT27700.12311412056739165No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT27580.12258077419670257No Hit
AGGAAGATGATGAGGTTCTTGGCGGCTGTCTGTGCAGGCTGCAGCTTCTT27130.1205807253066186No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG27080.12035849765216482No Hit
ATGGCATTGTATGAGAGCATGAGGGAACGCAGGTTGGACAGGTGGGCAAA26390.11729175602070274No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT26100.11600283562487083No Hit
GCCTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC26010.11560282584685404No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC25870.11498058841438345No Hit
CCAGCCTACCATACCTTTCACATTGACATTCATGATAGACTCTCTGTGAG25850.11489169735260196No Hit
CACTTAATTTTGTTTCTGATCTCACCATCCAAGCCATTCAGATCGGAAGA25590.11373611354944231No Hit
GAGGATCCCATGCCCTAATCGCCAATTCTGACCCATTCCTCGATGTCTAT25530.1134694403640978No Hit
CCCTCTTTCACTCAGCTTCACTCCACCATGACCAGAGCACCTCTCCTGCT25440.113069430586081No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG25240.11218051996826589No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA25060.1113805004122323No Hit
GTCTCTCCTCTCCTCCCAATTCCCATCTCCCTCTGCCCTCAGCCTTGACT24810.11026936213996343No Hit
CTCTGCAGTGATCACTTCCTCCTCCACCCGGGAGTACACGGTGACTGAGC24750.1100026889546189No Hit
GCTGAGTTTGAATGCATCCACCCTGAGAAGCAGCAGAAAAAGAAAAGCTA24710.10982490683105588No Hit
GGCCAGCTTCAAGTGTCAGGGCACTGTTGGAGCAGACTTACCGCCTGCAG24550.10911377833680377No Hit
AGAGAAGCTGCTACTCTTTTCACTCGAGCCATGGGGAGAAGCGCTGCCAA24140.10729151157028283No Hit
GCCCAGCTCACTGCCTTACCGTGCCCCTTAGAGCCACTGCTGGTGAAGCT24110.10715817497761056No Hit
CCCTTCATGGAATTAAGCATAAACTTAGATATATTGATCTACATAGTAAT24070.10698039285404755No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGTATTTCTGAG24000.10666927413781227No Hit
CAGGTGACATCGGCATCTGGTGGGGAGACTTCACACCGGAACGTGGCATC23890.10618037329801396No Hit
GCTGACCTGATCTTTGCTCTCCCCCTGGCCAGTTGAGGAGGAGAACCCGG23830.10591370011266941No Hit
GGAAGGGACAGCATGTGAGGCCCACACTCCTTGCAGAACTTGGGAAGGCT23560.10471367077861903No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT23520.104535888655056No Hit
GATGAGCCATTCCTGAAGAGAGCCTGCCAGGACGTCTCGGTCATCATCCA23500.10444699759327451No Hit
AGGAATGACCACAGTTCAGTGCCCTGATGGTGTGACATTCACATTCAGCA23370.10386920569169468No Hit
AACGATATTTGGCATAGCAACAGCCAGCACAAGATAACCAGCCACAGAAG23110.10271362188853506No Hit
GTCTGTTTTTAGACATTGCCATCGGAGTGCCTTTTGCAGGCAAGGATCAA23050.10244694870319053No Hit
AGCCTTTACGAATGTACTAAAGAGAAAATTATGTTTTAACTTTTCAGCAA22730.10102469171468637No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC22580.10035800875132503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)