FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249945
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA159130.7072617330645861No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC139470.6198818193333615No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA136150.6051259030776307No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC120860.5371686863456663No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG119830.5325907966639185No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG109090.48485629648724743No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC94430.4196991482014005No Hit
GCGGGACCACAATCCTTCGCTTCTCTCCAATGCAAACACCAAGTAGACCT93630.41614350573014003No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC90900.4040098757969639No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA89020.395654115989502No Hit
GCAGAAGAGAAGGGATCCATCCTAAGCATGACTCCTGAGCAGCTGGGCTC86780.3856983170699728No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA77450.3442306367488983No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT71150.3162299522877226No Hit
GAGTCTCAAAGCCATGTTATTCTGCCTTTTTAAACTATCATCCTGTAATC70010.31116316176617653No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC65600.2915626826433535No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC63320.28142910160026136No Hit
CCTGCTGTGGGATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCT58350.2593396727475561No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC55200.24533933051696818No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC53310.23693912517861546No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT52230.23213900784241392No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT50830.22591663351770822No Hit
GTAGTCGCCATGGAGGTAACTGGAATGATACGCGCCGTGGTGGTCTTGGT49840.22151652595952345No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT48720.2165386264997589No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT47820.21253852871959095No Hit
CCATTCTAATAGCCTCAAAATCTCTGCACCAGGGGGATGAAAGAATGCAT46100.20489389740638106No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG44880.19947154263770892No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC44290.19684925631515438No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC43070.19142690154648226No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT40330.17924882608241535No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA39600.17600430232739023No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA38270.17009304671891978No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG37000.1644484642957939No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG36220.160981712886315No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTTC36150.16067059417007973No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC34330.1525815075479623No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT34250.15222594330083625No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC34050.15133703268302112No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA33580.14924809273115566No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG32860.1460480145070213No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC32670.14520354942009694No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTGATCATTT31540.14018120442944162No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC31510.14004786783676934No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC31160.1384922742555929No Hit
ACCAGCAAGATCATGGTATCTGTATGGGCCTTCTTCGCTGTCATATTCCT31090.13818115553935764No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG30600.13600332452571062No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC29780.13235879099266873No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG29700.13200322674554268No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG29370.13053652422614775No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG29230.12991428679367717No Hit
GGCCAGCTTCAAGTGTCAGGGCACTGTTGGAGCAGACTTACCGCCTGCAG28480.12658087197687054No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT28200.1253363971119294No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT28160.1251586149883664No Hit
CATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCA28150.12511416945747564No Hit
CAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACA28010.12449193202500505No Hit
GCCCCAAGCTCGTATCCCGGAGGGCAGGGCCAGGGTCTTGGCAGAGCCTT27970.12431414990144204No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC27820.12364746693808071No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC27750.12333634822184543No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC27670.12298078397471937No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTGATCATTTC26930.11969181468880351No Hit
AGGAAGATGATGAGGTTCTTGGCGGCTGTCTGTGCAGGCTGCAGCTTCTT26690.11862512194742537No Hit
GGTTAGGTAGGAGCTTACTCAGTGCATCTGGAGGATGGAAAACCAATGGC26600.11822511216940859No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG26520.11786954792228255No Hit
GAGGATCCCATGCCCTAATCGCCAATTCTGACCCATTCCTCGATGTCTAT26450.11755842920604725No Hit
CGAGTGCGATGACCAGAGCGACGAGGAGGGCTGCCCCGTGTGCTCCGCCG26240.1166250730573414No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC26220.11653618199555989No Hit
ACCTTACCTTTTTGTCACTGAGGCCGGTCACTTGGTCCACAAATTCCTCT26180.11635839987199686No Hit
CCTGGCCTCCTCTTGATGGCACAGGACCTTCCCCATCCCGCCCCTGGAGC26080.11591394456308933No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT25930.115247261599728No Hit
GTCGACAGACAGGATTATCGTCTCCATCTTTCTCCAAAATAAGATTGGTC25860.1149361428834927No Hit
GCCCAGCTCACTGCCTTACCGTGCCCCTTAGAGCCACTGCTGGTGAAGCT25810.11471391522903894No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA25590.11373611354944231No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG25020.11120271828866927No Hit
GGAAGGGACAGCATGTGAGGCCCACACTCCTTGCAGAACTTGGGAAGGCT24970.1109804906342155No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC24860.11049158979441721No Hit
GATGAGCCATTCCTGAAGAGAGCCTGCCAGGACGTCTCGGTCATCATCCA24670.10964712470749285No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT24510.10893599621324077No Hit
GAGTCATCGTGGACGCATTCCTGGAAGGTGATGGTCTGGCGCCACTGGTA24320.1080915311263164No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA24140.10729151157028283No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT24090.10706928391582907No Hit
GGTGATAGCAGTTGGTGTCATGGAGCTCAGGAATCAGAGCCTTGGTTACA23920.10631370989068621No Hit
CCAGCCTACCATACCTTTCACATTGACATTCATGATAGACTCTCTGTGAG23740.10551369033465262No Hit
ACCCATTTTGTGACAGATGCTGCCTCTCCCTCAGGCCCTTCACCTTCGTG23490.10440255206238375No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAACCTC23260.10338030485189638No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG23190.10306918613566109No Hit
GGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCCA23110.10271362188853506No Hit
CGTGAGCGTTTACCAATATGGCAGTGCTTTGGCTCATTTCTTCTACAGCT22910.10182471127071996No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC22880.10169137467804767No Hit
GCTGACCTGATCTTTGCTCTCCCCCTGGCCAGTTGAGGAGGAGAACCCGG22870.10164692914715692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)