FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275598
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG236031.0372218643187416No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC154310.6781074688938907No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC95100.41791212683435297No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA74920.3292321402989456No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA62070.2727634670095509No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA60970.2679295727980074No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG58990.25922856321722904No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC54270.23848676260042417No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC52660.23141169925443772No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT51620.2268414719998875No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT50700.22279857865932384No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA49740.2185799073474313No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT48140.2115487884942771No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC47460.20856056298168657No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT47000.20653911631140473No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG46270.20333116833465312No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC46240.2031993348561565No Hit
CTGAGGAGCAGTAAGGGATGGCATCAGCAACGCGAGCCACAGGCAGGCCT45730.2009581657217136No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC44810.19691527238114992No Hit
GCCTGTTTCTCCATCTGAACTGCGCTCCTGGCCGCCCATGGCCTCCCAGT41770.18355614656015692No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC41600.1828090901820093No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC41200.18105131046872075No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA40190.17661291669266715No Hit
CAGGATTCCCTCTACCTACCTTCCCCCCATTTAGGCGTGCATTAGGCCTC39060.171647189002627No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC38360.16857107450437203No Hit
GGTGGAGGCCATAGGAATGGGAACATCATGGGCCACATCCCCTTGCCCTT37950.16676935029825127No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG37850.16632990536992914No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC37820.1661980718914325No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA37060.16285829043618424No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG34780.1528389460704395No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA34010.14945522012235904No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG33960.149235497658198No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG33950.14919155316536575No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC33490.14717010649508394No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG33450.14699432852375507No Hit
CATACACCCTCATCCTGCATCTCATCCACAGGATGAATTCTGCCGCCGGC33390.1467306615667618No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG33350.14655488359543292No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG33190.1458517717101175No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC33080.14536838228896318No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA32860.14440160344665445No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC31830.13987532068493644No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA31690.13926009778528545No Hit
GCAACTTGCATACCAGTGTTTCCTCAGCACAGGGCCAGGATGCAGCCCAG31650.13908431981395658No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT31600.13886459734979553No Hit
GCTTTGTATATGAGGCCTGTGGGGCAAGAACCTTTGTGCAGCGTTTCCGC31450.13820542995731233No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG31310.13759020705766134No Hit
CTGCGATTTTTCTGCATCAGGCCCAGCAGCACTGTACAGCAGGGTTCACC30910.13583242734437276No Hit
GCTCCAAATTTCATATTCGTCGAAAGCGATATGCATTGACAGGACAGAAA30470.13389886965975537No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA30220.13280025733895No Hit
CGGAGCATGTAGGTGTAGAAGGAAGCACCGAAGAGCAGCAGCACTATGAT29950.13161375603248027No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT29860.13121825559699032No Hit
CAGCTGTCTTCTTCCAGTACTGGACATTGGAGTTGGGGAAGTCATGGCTT29700.1305151437116749No Hit
GTGCTCCTTCATTTTTGATCTCCCCACTAGGTGCAATACCCTGATTATCA29700.1305151437116749No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT29650.13029542124751384No Hit
CTATGCCTTCAACACCAATGCCAACCGCGAGGAGCCTGTCAGCCTCACCT29620.1301635877690172No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC29510.12968019834786285No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC29500.12963625385503064No Hit
GGCCAATTGTGAGGACATTCTCATGAACTTCCTGGTGTCTGCTGTGACAA29200.12831791907006423No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT29190.12827397457723203No Hit
AGAGGGCTCTTCTATACTTACCTGTCCCATCATTGTCTCCTTATACTGCT29070.12774664066324543No Hit
GCCAATGAGTTCGGCAACTCCTGGGAGGAGGTGGTGCCCGACTCTCCCTG29030.1275708626919166No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG28510.1252857490646415No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG28490.12519786007897704No Hit
GAAGCAAGGATGTAAAACTCAGTGATCACCAGGGCGATGAAGAGAAGACA28490.12519786007897704No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC28200.12392346978684285No Hit
GAAGCTCAATGTCTGCTTTCGGAACTACAACGCCAAGACCGAGAACCTAG28110.1235279693513529No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC28040.12322035790152741No Hit
ATCCTGAAACTGGCCTGTGCAGACACCTCAGCCAACGAGATGGTCATCTT27880.122517246016212No Hit
CCAGGTCTATATGAGAAACCTTCTCTCTCAGCCCAGCCGGGCCCCACGGT27830.12229752355205094No Hit
GTCTCCTATACCACAGGCTCTGGGCATCACCAGCGGCCCCAGGGAAAAAG27650.12150652268107108No Hit
GCAGAAGATACTAAACTATGTGTCAAAACAAATAGTAATGAAATATGGGG27520.12093524427425231No Hit
CCAGCGATAGGAGGTCTTGTCCCGCCAGCCCACATTTCGTGGGTCTGTCC27440.12058368833159461No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG27440.12058368833159461No Hit
GTGAACAGCATAATCGCGTATTTCGTAAAGTCTGCTCCAAAGATGGCCTC27260.11979268746061475No Hit
GGTTGTCTACCTGAGCTACATGGCGGTGGAGTACAATGTGTCCTTCCCCC27150.1193092980394604No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG26860.1180349077473262No Hit
GGAACAGACGATGGACACATAGGACAGCACTATCAGGACAAAGCAGCCCG26560.11671657296235978No Hit
GGTCTTGGTAAGGACGTTCCCCACACAGATGGTCCCAGGCCTGAGGTCCC25920.1139041254210981No Hit
CCTCTCCTATGCCTTCATCGTGGCCGACATCTTGAAGATCCCTTCTGCTG25880.11372834744976924No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC25870.11368440295693703No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA25710.11298129107162162No Hit
ATCCTGGCTCAGCAACCTACCCTTTTCTCCCTGGGACTCCTTGTCTGCTG25510.11210240121497733No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT25130.11043251048735321No Hit
GGACTCCCTCAGTGCATTGCCTGAAACTCAGCATGACTTCACCTCCATCC25110.11034462150168879No Hit
TGCCTCTGCAGGTAATTTTGGAAACCTGATTGATGGAGGATCCCATCATG24810.10902628671672238No Hit
GTTTCCCCATCGCTTTCTTTGCCTGGCCTGTCACTCGTCTCTGCCTCCTC24680.1084550083099036No Hit
GGATCCTCCTTAACCTCCTACCCACCTCTGGCTCTCTGGGCCTGCCCGAG24650.10832317483140697No Hit
CCTGCTCTGAGTCATCCTCTTCATGCTGCCAGCAGTCTAGCTGCCAGCCG24640.10827923033857474No Hit
CTGCTGGAAGAATAAACACCAGGGCTAACACCATTGCCTGGCTCTCTTTC24540.10783978541025259No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA24510.10770795193175596No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAAC24490.10762006294609153No Hit
CATAAGCATAGCACCTGTACCACCACCTGCGACTCGGGCTCACGGGGCCC24420.10731245149626603No Hit
CATTTTGGGCACAATGACTAGGTTGAAGCCAATCTCCAGGAAAGATAAGT24250.10656539511811841No Hit
CAGAGCCTATACTGGGACCAAGGCCTTAGCCCAAGGGACACTCACCTCCT24190.10630172816112511No Hit
ATTCATGTCTGTCCTTCAAGCAGGAGAAAGGAGAGATTCTTCCCATTTGT23960.1052910048259842No Hit
GAGAGGGCAATTAAGACTTCTTACTCCTCGTCCTGAAGGGTCTCCTCCTC23850.10480761540482984No Hit
GAAGGGTCAGATCCTATCCACCTCCCTGGCCCCAGCTTAGTCCTTTCAGG23780.10450000395500435No Hit
GTCTCACACCCATCTCTCCTCTGCCCTCCAGCTCCCCCGCCTCCAAAGCG23770.10445605946217214No Hit
GGAGAACCAGAAAATGATGAACCAAGATGCCAGAACCAGGAGGCCACAGA23740.1043242259836755No Hit
CACAATGTTAACAAAACTCCCTATTTTCCCCACCGCATAGTAGCTCAGCA23660.10397267004101779No Hit
CTCACTTGGTCTTTGCATCCCACGCCTCATGTTACCTAAGGAGGCCAGCC23640.10388478105535337No Hit
CCTTTGGGCTTGAGGTAGATGACAGAGGCAAAGCCATAGTGCACAATGAC23540.10344533612703123No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGTCAGGGTGGCCATCCCC23420.10291800221304466No Hit
GGATCCTCCTTAACCTCCTACCCACCTCTGGCTCTCTGGGCCTGCCCAAG23420.10291800221304466No Hit
GCCTTGGAGGTGATGGAGATGGCCCCTGCCTGGCCCACCGCCTCCTGAAA23360.10265433525605137No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG23280.10230277931339367No Hit
ACATCCTTTTCATTTACTGGGACTGAGAGTGTACAGACAGTTATTGATGC23250.10217094583489703No Hit
GCACCACGAAATAGTTGTTCCAGACGTACAGCTGGTTGTCGCGAGGGTTG23160.1017754453994071No Hit
GGGTCCTCCTGGATGCTAATCTGGATCAGGCTCCGGCCACGGATTGTACA23150.10173150090657487No Hit
CCTCTACTTCATAGTGCTTATTGGCGGGCTGGTGGGTGTCATTTCCATTC23150.10173150090657487No Hit
GATATAAACATGAGCCCTGAGAACCAGAGCAGCGTGTCCGAGTTCCTCCT23120.10159966742807824No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT23090.1014678339495816No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC23070.10137994496391717No Hit
GCAGGGAGCCTACGTTCATGGGCCTCCCCTTCCCTGGATAGATGGTACAT23070.10137994496391717No Hit
GGTACACAGCAGATGGACTTGCAGCAGACAGGCTTGCAGCAGACGGACAC22910.10067683307860176No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG22910.10067683307860176No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC22800.1001934436574474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)