FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275598
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG223110.9804455795795215No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC141230.6206280722693551No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC94520.4153633462500846No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG59150.2599316751025445No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT54570.23980509738539055No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG46110.2026280564493377No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT44020.19344365744740502No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG43180.18975232004949907No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG43170.18970837555666686No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC42900.1885218742501971No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC42660.18746720642222398No Hit
GGCGAGTGTACCAGTACCTGGCTGCCGACTGCACCTCCACCTTCCCTCGG40520.1780630849561302No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA39290.17265791233776792No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC38630.1697575758108418No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC38580.16953785334668073No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC38370.16861501899720424No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC38190.1678240181262244No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA37760.16593440493443923No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC37600.1652312930491238No Hit
GGAGCAGCTTGGTAACTCTGGCTCCCATTTGTTCAGGGCCTGGCACTTCA37460.1646160701494728No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT37320.1640008472498218No Hit
CGGAGCATGTAGGTGTAGAAGGAAGCACCGAAGAGCAGCAGCACTATGAT37060.16285829043618424No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC36260.15934273100960716No Hit
CCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCC35350.1553437821618757No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA34530.15174033374963417No Hit
GCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAG34230.15042199896466776No Hit
GAAGCAAGGATGTAAAACTCAGTGATCACCAGGGCGATGAAGAGAAGACA33510.14725799548074836No Hit
GCCAATGAGTTCGGCAACTCCTGGGAGGAGGTGGTGCCCGACTCTCCCTG33180.1458078272172853No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC32750.14391821402550012No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA32680.1436106025756746No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC32230.141633100398225No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA32180.14141337793406392No Hit
CACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCGGAG32140.14123759996273508No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG31960.14044659909175522No Hit
CAGGATTCCCTCTACCTACCTTCCCCCCATTTAGGCGTGCATTAGGCCTC31840.13991926517776865No Hit
GGCAGTTCCACCATCAAGTACACCACCACCTCCTCCTCCAGCAGGAAGAG31740.13947982024944652No Hit
GCACCACGAAATAGTTGTTCCAGACGTACAGCTGGTTGTCGCGAGGGTTG31690.13926009778528545No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG31310.13759020705766134No Hit
GCAGAAGATACTAAACTATGTGTCAAAACAAATAGTAATGAAATATGGGG31200.13710681763650698No Hit
CAGCTGTCTTCTTCCAGTACTGGACATTGGAGTTGGGGAAGTCATGGCTT30480.13394281415258757No Hit
GCAACTTGCATACCAGTGTTTCCTCAGCACAGGGCCAGGATGCAGCCCAG30050.1320532009608024No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC29820.13104247762566146No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC29690.1304711992188427No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT29490.1295923093621984No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA29370.12906497544821185No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA29280.12866947501272194No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT29160.12814214109873537No Hit
CGCCCCTCAGTGCATTATACCTAACAAATGCACGCCTCCAAATGTGGAAA29020.12752691819908438No Hit
GGCCAATTGTGAGGACATTCTCATGAACTTCCTGGTGTCTGCTGTGACAA28910.12704352877793001No Hit
AGAGGGCTCTTCTATACTTACCTGTCCCATCATTGTCTCCTTATACTGCT28890.1269556397922656No Hit
CCACCTCTCCTTTGTAGACATTTGCTATTCTTCCAATGTTACTCCAAATA28730.12625252790695016No Hit
GGAACAGACGATGGACACATAGGACAGCACTATCAGGACAAAGCAGCCCG28650.12590097196429245No Hit
CAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGGTG28580.12559336051446698No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT28570.12554941602163475No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC27900.12260513500187643No Hit
AGGAGTCATGGGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCG27740.121902023116561No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG27690.12168230065239993No Hit
GAGAAATTATTTAATACTTCAGCCAAGAAGTCTTTCTAGACTAAATTGTT27540.12102313325991673No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG27510.1208912997814201No Hit
GAGAGGGCAATTAAGACTTCTTACTCCTCGTCCTGAAGGGTCTCCTCCTC27310.12001240992477581No Hit
GAAGGGTCAGATCCTATCCACCTCCCTGGCCCCAGCTTAGTCCTTTCAGG27030.11878196412547382No Hit
GGAGAGCCAGCCATTGTGGGGGCAGCTCCACAGATAGGACTCATGTCCTG26660.11715601789068192No Hit
GCAGGGAGCCTACGTTCATGGGCCTCCCCTTCCCTGGATAGATGGTACAT26550.11667262846952757No Hit
ATCCTGAAACTGGCCTGTGCAGACACCTCAGCCAACGAGATGGTCATCTT26420.1161013500627088No Hit
GCTCCAAATTTCATATTCGTCGAAAGCGATATGCATTGACAGGACAGAAA26420.1161013500627088No Hit
GCAGCATGATGAGCAGGTTCCCCAGCACCGTGGTCAGGTACATGCCCAGG26130.1148269597705746No Hit
GGTGGAGGCCATAGGAATGGGAACATCATGGGCCACATCCCCTTGCCCTT25900.11381623643543368No Hit
GGACTCCCTCAGTGCATTGCCTGAAACTCAGCATGACTTCACCTCCATCC25830.11350862498560818No Hit
TGCTGTAACAAAGGTGACTGTGGCACTCCAAGCCATTCCAGGAGGCAGCC25650.11271762411462834No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT25530.11219029020064177No Hit
TGAAAAGCAAGGCTGTATAGAAGACGGTGACCACGGTGAGGTGAGAGGCG25480.1119705677364807No Hit
CTGCTGGAAGAATAAACACCAGGGCTAACACCATTGCCTGGCTCTCTTTC25430.11175084527231963No Hit
GCACTGTCAGCAGAAAAACGCTGTGGACCACCACCAAGTTAATGACCGCC25260.11100378889417198No Hit
CATACACCCTCATCCTGCATCTCATCCACAGGATGAATTCTGCCGCCGGC25130.11043251048735321No Hit
GGAGAACCAGAAAATGATGAACCAAGATGCCAGAACCAGGAGGCCACAGA25120.110388565994521No Hit
CCTTTGGGCTTGAGGTAGATGACAGAGGCAAAGCCATAGTGCACAATGAC24900.10942178715221229No Hit
GAAGCTCAATGTCTGCTTTCGGAACTACAACGCCAAGACCGAGAACCTAG24890.10937784265938008No Hit
CTGAGGAGCAGTAAGGGATGGCATCAGCAACGCGAGCCACAGGCAGGCCT24850.10920206468805123No Hit
CAGACACACGGAGCATGTGTTTGCCCTGCGGCCGGTGGGATTCCGGGACA24740.10871867526689688No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT24380.10713667352493718No Hit
GCCACCAAGCCTCATGTGCTGCCAGCTGTCCTGGCAGAGGTGGCTCCTGT24280.10669722859661505No Hit
GGATCCTCCTTAACCTCCTACCCACCTCTGGCTCTCTGGGCCTGCCCGAG24230.10647750613245396No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT24050.10568650526147413No Hit
GGACTCCCTCAGTGCATTGCCCAAAACTCAGCATGACCTCAGCTCCATCC23950.105247060333152No Hit
CCAGGTCTATATGAGAAACCTTCTCTCTCAGCCCAGCCGGGCCCCACGGT23930.10515917134748756No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC23830.10471972641916541No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC23790.10454394844783656No Hit
GGTACACAGCAGATGGACTTGCAGCAGACAGGCTTGCAGCAGACGGACAC23760.10441211496933993No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC23650.10392872554818558No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG23480.10318166917003793No Hit
CCTCTCCTATGCCTTCATCGTGGCCGACATCTTGAAGATCCCTTCTGCTG23360.10265433525605137No Hit
GAGGCCAGGAAGTTTGACCGCGCTGCCATGCCGAACCGCAAGGCCAGCCG23310.10243461279189031No Hit
GGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGCC23230.1020830568492326No Hit
CCGGCATGGTGACTGTACAGGGCTGGGCCGGGTGTGCGACTTACCGAGCT23190.10190727887790375No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC23110.10155572293524602No Hit
GGTTGTCTACCTGAGCTACATGGCGGTGGAGTACAATGTGTCCTTCCCCC23080.10142388945674939No Hit
GTTGCTGCTTCAGGAATGGAGGGAGTGAGAGCCTTTTCCGACAATGCCGA22860.1004571106144407No Hit
CCTGCTCTGAGTCATCCTCTTCATGCTGCCAGCAGTCTAGCTGCCAGCCG22850.10041316612160849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)