FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146534
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG164110.7645348268417831No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC115950.5401731349235559No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC106760.49735993000809675No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA93180.43409515060092224No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA82570.38466663001843904No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC69940.3258275899659637No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT57700.2688054323854176No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT53830.25077636785627433No Hit
CACTTAATTTTGTTTCTGATCTCACCATCCAAGCCATTCAGATCGGAAGA49920.23256095640693322No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC45420.2115969278846736No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC44730.2083824435112605No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC43000.2003229392127029No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT41850.19496546525701433No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC41780.19463935814666805No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT41690.1942200775762229No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA41320.19249636856439264No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA40750.1898409249515731No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC40640.18932847092102897No Hit
ATACACCCCCATAATGCTCAGAATTTCTTAAATGTAATTTACTGAGATCG39400.1835517163948952No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC39270.18294608890425218No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG38270.17828741589930558No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT37830.17623759977712908No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG36870.17176527369238037No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA36650.17074036563129213No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA36500.17004156468055012No Hit
CAGTAAATTACATTTAAGAAATTCTGAGCATTATGGGGGTGTATAGATCG36490.16999497795050067No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC36300.16910983007956082No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA36000.16771222817807685No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG34910.1626342746026851No Hit
CCGTGTCCAAGGAGGTCATGTACACAGCCTTCAGATCGGAAGAGCACACG33940.1581153617878869No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA33010.15378279589328658No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG32890.15322375513269298No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT32420.15103417882036807No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCCGGCCAGGGTGGCCATCCCC31960.14889118923809266No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC31660.1474935873366087No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT31500.14674819965581726No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC30930.14409275604299768No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC30370.1414838991602276No Hit
CTGGGTTGGGCATGTGAAATTCCATTTTCTGTTTGAGATCGGAAGAGCAC29040.13528786406364868No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC28660.13351756832176895No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG27540.12829985455622878No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC27370.1275078801453879No Hit
GCAGGTACCCTCAACTGAGTAAGGGACAATGAACAGCCTCTTGGCCATTC27290.12713518630499215No Hit
GTTTAGGGTCATGGGTTCTTGTTCTTTATTGTATCGGAGATCGGAAGAGC26530.12359459482123275No Hit
AGAATCCAGCTTGTGTTCCAGCTCTTCCCATCAGATCGGAAGAGCACACG26440.12317531425078754No Hit
GAAGGCTGTGTACATGACCTCCTTGGACACGGAGATCGGAAGAGCACACG26260.12233675310989717No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT26040.12131184504880892No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA25950.12089256447836373No Hit
TGTGATCTTAACCTGTGATCCTGTCCATCATCTACTTCAGATCGGAAGAG25890.12061304409806692No Hit
TGTCACCTTATACTTCTAGTTTGCTTATTATCGCCGGACAGATCGGAAGA25670.11958813603697867No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC25410.11837688105569258No Hit
GAAGTAGATGATGGACAGGATCACAGGTTAAGATCACAGATCGGAAGAGC25160.11721221280445594No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC24820.1156282639827741No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT24780.11544191706257623No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC24770.11539533033252676No Hit
GTCCGGCGATAATAAGCAAACTAGAAGTATAAGGTGACAAGATCGGAAGA24560.11441700900148798No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG24500.11413748862119119No Hit
GCCTGCAGGAACCATCATCACACTGGAAGACAGATCGGAAGAGCACACGT24390.11362503459064707No Hit
GAAGTAGATGATGGACAGGATCACAGGTTAAGATCACAAGATCGGAAGAG24330.11334551421035026No Hit
GATATCAGAGTCTCACTGGTCTCCATCAACGCAGAGATCGGAAGAGCACA24080.11218084595911362No Hit
CTGCGTTGATGGAGACCAGTGAGACTCTGATATCAGATCGGAAGAGCACA23990.11176156538866844No Hit
GCGGGACCACAATCCTTCGCTTTTCTCCAATGCAAACACCAAGTAGACCT23750.11064348386748124No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG23300.1085470810152553No Hit
CTGAGGAGCAGTAAGGGATGGCATCAGCAACGCGAGCCACAGGCAGGCCT23030.1072892393039197No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT22900.10668361181327667No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA22850.10645067816302933No Hit
GGTTAGGTAGGAGCTTACTCAGTGCATCTGGAGGATGGAAAACCAATGGC22560.10509966299159482No Hit
GTCTTCCAGTGTGATGATGGTTCCTGCAGGCAGATCGGAAGAGCACACGT22520.10491331607139695No Hit
CCGCATTAATATCCCAGAGAGAGCAGGTGGACCAGAAGGCAACTTAAACC22450.1045872089610507No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG22360.10416792839060551No Hit
GATGGGAAGAGCTGGAACACAAGCTGGATTCTAGATCGGAAGAGCACACG22270.10374864782016031No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG22140.10314302032951726No Hit
GCCTGTTTCTCCATCTGAACTGCGCTCCTGGCCGCCCATGGCCTCCCAGT22070.10281691321917101No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG21770.10141931131768703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)