FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001129731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402124
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA208350.8673573887109909No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA186020.7743979911111999No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC147790.6152471729186337No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC121300.5049697684216136No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA83460.3474425133756625No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT74380.30964263293651784No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG73450.30577105927920456No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT70990.2955301225082469No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC69240.28824490326061436No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT68910.2868711190596322No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG67590.2813759822557037No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC65370.27213416126727846No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC64220.2673467314759771No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG63110.26272582098176445No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT59170.24632367021852328No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT54150.225425498433886No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC50820.21156276695124815No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC49270.20511014418905937No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC48650.20252909508418382No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG48190.20061412316766328No Hit
CTCTGCAGTACTCCTCATCCTGCATTTGGTCTCCAGGTGTCACCTTTCTG47740.19874078107541493No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG47590.19811633371133214No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT46760.1946610582967407No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC45620.18991525832971154No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA44840.18666813203648105No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT43940.18292144785198433No Hit
GCCACATCCCTGGGCTTATGGTGCAGCAGGCGCTTTTTTCAAGACAGGAA43670.1817974425966353No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA42370.17638556544125114No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT41910.1744705935247306No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC41460.17259725143248225No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG41270.1718062847713107No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC40880.17018272162469547No Hit
GGTTAGGTAGGAGCTTACTCAGTGCATCTGGAGGATGGAAAACCAATGGC39760.16552018130621066No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT38900.16194001641880268No Hit
GGTGAACTCCTGGAGGATCTCCTGGCGGAGGTGGTGGTAGAAGGTCCAGG38750.16131556905471992No Hit
ACTGTGCTCACCTTCTTCATGTCCTCATAGAAGAGATAGAGAATACGGTG38500.16027482344791527No Hit
AAGTGGTGACATCAGAACCTGCCCTCCACACCCAACAGCTGGACTGCAGC37620.1566113989119629No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCGCCTCGCTTTATGATGTGTA37560.1563616199663298No Hit
ATGATGGTCCTTCATGTGTTGTAAGAGGACCTGATCTGTTTCAAATGACA37280.15519598488670858No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGCATTTCTGAG36780.15311449367309932No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC36160.1505334445682238No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA35910.14949269896141915No Hit
AGGTGTTGACTGCGATATTGACGTTCCCAAAACCATCCAGATGGTGCGGT35810.1490764007186973No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTGATCATTT35150.14632883231673302No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA35140.14628720249246085No Hit
CAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACA35130.14624557266818866No Hit
CTCTGCAGTGATCACTTCCTCCTCCACCCGGGAGTACACGGTGACTGAGC34610.144080821806035No Hit
CATCTAATTTCCCGGGCCATACCCTGCAGGTACTGGATGATGCTGAGGTT34200.14237399901087536No Hit
ATGGCATTGTATGAGAGCATGAGGGAACGCAGGTTGGACAGGTGGGCAAA33970.1414165130526151No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC33630.14000109902736077No Hit
AGGAACAAAAGATACTCACAGAGGACATTCAGGGTGACTGGGTCACTGCG33480.139376651663278No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC32880.13687886220694687No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTGTATGATGTGTA32800.13654582361276937No Hit
CCAGCCTACCATACCTTTCACATTGACATTCATGATAGACTCTCTGTGAG32560.13554670783023692No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTATACCTCACTGTGGTGTC32420.1349638902904263No Hit
GCTGACCTGATCTTTGCTCTCCCCCTGGCCAGTTGAGGAGGAGAACCCGG32200.13404803415643823No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC32170.13392314468362165No Hit
TGCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCA32000.1332154376709945No Hit
CGTGTTGTGCAGTCTCTTTCTGTGCAGGTCGTCCTTCCGGACCAGCACCA31740.1321330622399177No Hit
AGGAAGATGATGAGGTTCTTGGCGGCTGTCTGTGCAGGCTGCAGCTTCTT31500.13113394645738521No Hit
CCCTCTTTCACTCAGCTTCACTCCACCATGACCAGAGCACCTCTCCTGCT31410.13075927803893553No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT31360.13055112891757462No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC31180.12980179208067527No Hit
GCCCAGCTCACTGCCTTACCGTGCCCCTTAGAGCCACTGCTGGTGAAGCT30810.1282614885826044No Hit
ACCTCCTTGGCGTAGTTCTGGCACTCGGCGCTGGCGGCCGCCATGTGCTT30620.12747052192143288No Hit
GAAATATCCAGTTACTCCTCTAGTCCTATCACCTCGCTTTATGATGTGTA30590.1273456324486163No Hit
GAGTCATCGTGGACGCATTCCTGGAAGGTGATGGTCTGGCGCCACTGGTA30590.1273456324486163No Hit
CCTTCCCCGACCCTTCTATCCACCTACCTCATGCATGGTGAGGTCAGAGA30520.127054223678711No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG30480.12688770438162225No Hit
AGGAACAAAAGATACTCACAGAGGACATTCAGGGTGACTGGGTAACTGCG30190.1256804394777289No Hit
GCCACATCCCTGGGCTTACGGTGCAGCAGGTGCTTTTTTCAAGACAGGAA30160.1255555500049123No Hit
AGGAATGACCACAGTTCAGTGCCCTGATGGTGTGACATTCACATTCAGCA30150.12551392018064014No Hit
GAGTGCCCTTTGAGCAGAGGCTGCTGGCGCATGCGTGTGCGTGTCTGTGC30110.1253474008835514No Hit
CTGCTGGTCAGTTGAGTTTCTCGGTCTGGCTCCTGTGCAGAGAGGGCCTC30020.12497273246510172No Hit
AGCTTCTCCACAGTGTAGACTGAAACAAGGGAAACATGAACATCACAAAC29820.12414013597965799No Hit
ACCACGTGAAGGTGATTGCTGGCCGATTCGAGGGCGACACAGGCCTCATT29750.1238487272097527No Hit
GCTGCTGACAAACACGTCTTCTGCAGCTGGCCTGCAGTCTGCTAACACTG29730.12376546756120833No Hit
GACACTTGCACTCAGGGTAGAAACAGAACAGGTTTTGGCTTGGTTATTAT29300.12197538511750435No Hit
GATGAGCCATTCCTGAAGAGAGCCTGCCAGGACGTCTCGGTCATCATCCA29120.12122604828060501No Hit
GAATGGGAGACTGTGACTCACTAAAGGCCAACCAGGTCTGCCGTAAGTGC28630.1191861868912679No Hit
CCTGGCCTCCTCTTGATGGCACAGGACCTTCCCCATCCCGCCCCTGGAGC28610.11910292724272352No Hit
CAGGTGACATCGGCATCTGGTGGGGAGACTTCACACCGGAACGTGGCATC28590.11901966759417916No Hit
GATTTCCACCACAACAAGCAACGCCACCTTTGATCATGGAAGAAAGACCC28320.11789566233883014No Hit
AATGCCAACTACCAGGTGTACGACAGCCTGGAGCTGAAGCGGCAGGTGCA28230.11752099392038046No Hit
CTGATGCATGACAGCCTGAGGCCACACCCCCCTGTTTCTGCAGGCCAGGG28160.11722958515047516No Hit
GCCACATCCCTGGGCTTATGGTGCAGCAGGTGCTTTTTTCAAGACAGGAA28080.11689654655629768No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCATCTCCCTTTATGATGTGTA28020.11664676761066456No Hit
CCTTACCTGCTCCAACCAAAGATGGGCGGGGCTGTCCACACAGCAGACCA27780.11564765182813211No Hit
GCCAATGATGAACCGGATGATGCCTCTCACCGTCAACATGGCAACGGCCA27660.11514809393686587No Hit
GGATACAAACAAGGTTCTGAAAGTTGTCGTGGTAGAGGACGCGGGCGTGG27640.11506483428832151No Hit
GCCTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC27470.11435712727569435No Hit
CATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCA27350.11385756938442812No Hit
AAAGCAGATCTCAATTAAGCAGACCAGCATTTGCACAGGATCACTCAGGC27290.113607790438795No Hit
AGGAACAAAAGATACTCACAGAGGACATTCAGGGTGACTGGGTTACTGCG27270.11352453079025063No Hit
GACAAGAACTTGAAGCATTTTGTGGCCTTCCACACCCACTTGAATTGCTA27190.11319149219607315No Hit
CTCTGTGTTTGTCCCTCAGACAAGTTGGATGGCTTGAGGACTGGTACTAA27050.11260867465626254No Hit
CACTTAATTTTGTTTCTGATCTCACCATCCAAGCCATTCAGATCGGAAGA26950.11219237641354068No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC26880.11190096764363539No Hit
AGGGAAGATACCTGTGGCTTAATAAACGAAAGTAAGGAGAGCCCTGGCGG26790.11152629922518571No Hit
CTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTAC26700.11115163080673604No Hit
GGAAGGGACAGCATGTGAGGCCCACACTCCTTGCAGAACTTGGGAAGGCT26680.11106837115819168No Hit
GGGCTAGGCTATAAGTGACCTGACCATTGGTGCCTTGGTCAGGATCCCGA26550.11052718344265325No Hit
CCTGCATATAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGAT26420.10998599572711483No Hit
GTCTCTCCTCTCCTCCCAATTCCCATCTCCCTCTGCCCTCAGCCTTGACT26400.10990273607857047No Hit
CAGCATGATTAACTGCACCAGGGAGGTGTCCACACAAACCAATTTCAAGA26360.10973621678148174No Hit
GTCTGTTTTTAGACATTGCCATCGGAGTGCCTTTTGCAGGCAAGGATCAA26350.10969458695720953No Hit
GAGGATCCCATGCCCTAATCGCCAATTCTGACCCATTCCTCGATGTCTAT26260.10931991853875987No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG26250.10927828871448766No Hit
CCCTTCATGGAATTAAGCATAAACTTAGATATATTGATCTACATAGTAAT26130.10877873082322145No Hit
GGTTTTATCCTCAGCTTCTTCATAGCTGTCTTCATATCAGTATTTCTGAG25980.10815428345913866No Hit
GCTGAGTTTGAATGCATCCACCCTGAGAAGCAGCAGAAAAAGAAAAGCTA25980.10815428345913866No Hit
GGCTTCAGGTACACGTAGATGATGGTTCCGTAGAAGAGAGAGACCACTGT25900.10782124486496117No Hit
ACAGTCCACGATGCCATTGCTGCAGGTGCACATGGCCGGGCAGGAGCCGG25840.10757146591932806No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGCGGGG25830.10752983609505588No Hit
GAAGCAGACCGTCTTCTACTTGGAAGATTACCTCACTGTCCGGAGGGGGG25800.10740494662223933No Hit
GGCCAGCTTCAAGTGTCAGGGCACTGTTGGAGCAGACTTACCGCCTGCAG25710.10703027820378964No Hit
GTTGCTCTCACAAACAGCTTCAGTGGGTTTCTGCCATCCTCTCTGCCTGG25700.10698864837951746No Hit
CCAGTGAAGGTTTTTGCCTGACCATGCCTTGGAAAGGGCTAGGGAGATTG25650.10678049925815652No Hit
CCAACCTGAACCGTGTGATTTATTTCATCTGTGACACCATCCTCTGGGTG25570.10644746066397905No Hit
GCAACTTCTTATAGCGGGTGAGGGCAGCGATAAAGACAAAGTTACCGATG25380.10565649400280752No Hit
CGGATGAGAAAGGAGTCGATGAAGTCCCGTGGGGAATTGGGATCCAGCGT25380.10565649400280752No Hit
GGCTCAGCAGAAGAGAATAGTAGTAGTGGTGAGCAGCCCTCGTTCGCCAT25240.10507367646299692No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC25210.10494878699018036No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT25200.10490715716590818No Hit
AGAGAAGCTGCTACTCTTTTCACTCGAGCCATGGGGAGAAGCGCTGCCAA25170.10478226769309162No Hit
GGTGGCGTGACCTGTAGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGG25170.10478226769309162No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCT25000.10407456068046446No Hit
CAATGCACCTGCCTTTGACCGCCAGCTCTACCGACCTGAGCCCCTGCCTG24840.10340848349210949No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC24790.10320033437074855No Hit
GCCATGGTGGAAATGATGTCTGTGCTGCCACTGTCTCTCTGTGGTAATAG24770.10311707472220419No Hit
CGCTCAGATGAGGATGAGACGGCGCGCGTGGCCACCTCCCGCTGTAGACC24650.10261751683093795No Hit
GGAACCAGTAACCAAGACCAAGCCGTGGAGGGAGCCACTGTGGGAGCGGA24550.10220121858821608No Hit
AATACCAACAGCAACATTATCTGAATACACACTTCTCATTGCCACTGCGA24390.10153514139986113No Hit
ATACACCCCCATAATGCTCAGAATTTCTTAAATGTAATTTACTGAGATCG24320.10124373262995583No Hit
CTTCTCCCTTATGTCCTTATGTCCCTCGGCACTTACCACATTTTTGGCAT24270.10103558350859489No Hit
AAACTGACAAAGTCCATCTGGAGGCGGGGGCCGCCGTAGCGGATGAGCAT24200.10074417473868959No Hit
ACTCTGAGTCTACAACCCACTCACGTGCCTTCAGCTTAGCCAACATCCGA24080.10024461684742336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)