FastQCFastQC Report
Wed 8 May 2024
EGAF00001129732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402124
Total Bases360.3 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC133340.5550920768453252No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC120340.5009733052914838No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA104410.43465699522589174No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA96430.4014363954566875No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT81270.33832558186005385No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA79910.3326639257590366No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT71050.29577990145388No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC69150.2878702348421647No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC67410.28062664541880433No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC67280.2800854577032659No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC63780.2655150192080009No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG63210.2631421192244863No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG62490.26014477187688895No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG61070.2542333368302386No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT56950.237081849230098No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGGGGGG51630.2149347827172952No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT51500.21439359500175675No Hit
GCCACATCCCTGGGCTTATGGTGCAGCAGGCGCTTTTTTCAAGACAGGAA51350.213769147637674No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT47190.1964511407404447No Hit
CAATGTCCACAAAGATGACCATCTCGTTGGCTGAGGTGTCTGCACAGGCC46370.19303749515012547No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC46100.19191348989477647No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA46010.19153882147632678No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT44930.1870428004549307No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC43560.18133951452964125No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT43190.17979921103157037No Hit
GACGTGTCTAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGC42410.1765520847383399No Hit
GGTGGAGGTGAGGCAGATGCCCAGCAGGCGGCACACGTGGGGGTTGTCCA42210.17571948825289618No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCGCCTCGCTTTATGATGTGTA41870.17430407422764188No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTTC40450.16839263918099148No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA40000.16651929708874313No Hit
CTCTGCAGTACTCCTCATCCTGCATTTGGTCTCCAGGTGTCACCTTTCTG39650.1650622532392166No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTGATCATTT39140.16293913220133516No Hit
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG38400.1598585252051934No Hit
ATGATGGTCCTTCATGTGTTGTAAGAGGACCTGATCTGTTTCAAATGACA38110.15865126030130003No Hit
AGGCCCTCATGAATGACCTCTTTCTTTCCTTTCTTCTTTTCTAGCTACTC38020.15827659188285034No Hit
GTGTGATCTGAACCCTTTGCTCAACCTCGCCTGCACTGACACCCATATGC36920.15369731121290992No Hit
GTCACATGTGATCCAGGTCTCCTCCGAGGGGCTGGCCAGTCTCTTCTCCC36730.15290634455173838No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT35910.14949269896141915No Hit
GAAGTGAGCTGCAGTCTCTGCTGATTGCCACGACCTGCCTTCGGGAGCAC35380.1472863182749933No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTGTATGATGTGTA35090.1460790533710999No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA34740.14462200952157342No Hit
GAGTCATCGTGGACGCATTCCTGGAAGGTGATGGTCTGGCGCCACTGGTA33640.14004272885163296No Hit
TGCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCA33480.139376651663278No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT33340.1387938341234674No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTACACCTCACTGTGGTGTC33310.13866894465065083No Hit
AGGAACAAAAGATACTCACAGAGGACATTCAGGGTGACTGGGTCACTGCG33000.13737842009821308No Hit
CCTGAAGTCTGGCTATGGAAAATATCTTGGTATAAATTCAGATGAACTTG32860.13679560255840248No Hit
GAAATATCCAGTTACTCCTCTAGTCCTATCACCTCGCTTTATGATGTGTA32520.13538018853314818No Hit
GGCAAGTGGAAAGCCTTCTCCACCTGTGGCTTATACCTCACTGTGGTGTC32380.13479737099333758No Hit
CAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTG32360.1347141113447932No Hit
AGAGAACCCTTACAAATTCGAAGAATGTGGTAAAGCCTTTTATTGGTTTT32330.13458922187197664No Hit
GCTGACCTGATCTTTGCTCTCCCCCTGGCCAGTTGAGGAGGAGAACCCGG32300.13446433239916006No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTGATCATTTC32040.13338195696808325No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCATCTCCCTTTATGATGTGTA31610.13159187452437926No Hit
GATGAGCCATTCCTGAAGAGAGCCTGCCAGGACGTCTCGGTCATCATCCA31460.13096742716029647No Hit
GAGTGCCCTTTGAGCAGAGGCTGCTGGCGCATGCGTGTGCGTGTCTGTGC31170.1297601622564031No Hit
GCCCTCATGATTCTCCTGATTCACCGGGACCACCGGCTCCACACGCCCAT30880.1285528973525097No Hit
TGGAGGCGTGCATTTGTTAGGTATAATGCACTGAGGGGCGGGGCCGCTCC30830.12834474823114878No Hit
GCCACATCCCTGGGCTTATGGTGCAGCAGGTGCTTTTTTCAAGACAGGAA30680.12772030086706598No Hit
ACCACGTGAAGGTGATTGCTGGCCGATTCGAGGGCGACACAGGCCTCATT30620.12747052192143288No Hit
AGGAACAAAAGATACTCACAGAGGACATTCAGGGTGACTGGGTAACTGCG30610.12742889209716068No Hit
GCCACATCCCTGGGCTTACGGTGCAGCAGGTGCTTTTTTCAAGACAGGAA30510.12701259385443883No Hit
GCCCAGCTCACTGCCTTACCGTGCCCCTTAGAGCCACTGCTGGTGAAGCT30450.1267628149088057No Hit
GGCCAGCTTCAAGTGTCAGGGCACTGTTGGAGCAGACTTACCGCCTGCAG29850.12426502545247456No Hit
CAACCCTGCATCTTTAGACAAGGCCATGTCCATTCCCATGTGGGTGGACA29690.1235989482641196No Hit
GCTGCTGACAAACACGTCTTCTGCAGCTGGCCTGCAGTCTGCTAACACTG29630.12334916931848647No Hit
AGGAAGATGATGAGGTTCTTGGCGGCTGTCTGTGCAGGCTGCAGCTTCTT29500.12280798160294805No Hit
CCAGCCTACCATACCTTTCACATTGACATTCATGATAGACTCTCTGTGAG29310.12201701494177652No Hit
CTGCTGGTCAGTTGAGTTTCTCGGTCTGGCTCCTGTGCAGAGAGGGCCTC29200.1215590868747825No Hit
CTCTGCAGTGATCACTTCCTCCTCCACCCGGGAGTACACGGTGACTGAGC29100.12114278863206063No Hit
GACACTTGCACTCAGGGTAGAAACAGAACAGGTTTTGGCTTGGTTATTAT28830.1200187833767116No Hit
GGTGAACTCCTGGAGGATCTCCTGGCGGAGGTGGTGGTAGAAGGTCCAGG28680.11939433601262882No Hit
CCTTCCCCGACCCTTCTATCCACCTACCTCATGCATGGTGAGGTCAGAGA28380.11814544128446326No Hit
CATCGTGTTTGGGAGTTTCCTGCTGCTGCTCTTCTCCCTGACCCAGTTCA28260.11764588339319701No Hit
AACAGAGAGAATTTTCACTATGGATCAGTGGTGACCTACCGCTGCAATCC28240.11756262374465265No Hit
GAAGCACGAGTATCATAATCATCGGGGAGCTGAAGATAATCCTCCATTCT28210.1174377342718361No Hit
ACCTCCTTGGCGTAGTTCTGGCACTCGGCGCTGGCGGCCGCCATGTGCTT28040.11673002725920893No Hit
GTGATGACCACCAGAGTCATGCAAATGAGCATCTTCTCAGTGATGGTCTT27920.11623046936794271No Hit
AGGAACAAAAGATACTCACAGAGGACATTCAGGGTGACTGGGTTACTGCG27810.11577254130094868No Hit
GGTTTTCTGCACTGCTTGTAAAACAGACTTGTAGATGCGGAAACTGATGG27790.11568928165240429No Hit
GGAAGGGACAGCATGTGAGGCCCACACTCCTTGCAGAACTTGGGAAGGCT27780.11564765182813211No Hit
CAGGTGACATCGGCATCTGGTGGGGAGACTTCACACCGGAACGTGGCATC27770.11560602200385992No Hit
CCAGTGAAGGTTTTTGCCTGACCATGCCTTGGAAAGGGCTAGGGAGATTG27760.11556439217958774No Hit
ACAGTCCACGATGCCATTGCTGCAGGTGCACATGGCCGGGCAGGAGCCGG27700.1153146132339546No Hit
GTCGACAGACAGGATTATCGTCTCCATCTTTCTCCAAAATAAGATTGGTC27670.11518972376113806No Hit
GAAGCAGACCGTCTTCTACTTGGAAGATTACCTCACTGTCCGGAGGGGGG27480.11439875709996652No Hit
GCCATCATTTCCTGGGAGAGGTGGCTGGTGGTGTGCAAGCCCTTTGGCAA27300.11364942026306718No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA26990.11235889571062942No Hit
GAGGATCCCATGCCCTAATCGCCAATTCTGACCCATTCCTCGATGTCTAT26990.11235889571062942No Hit
GGCATGACGAAGCTGACTGCGTGCCAGGTGGCCACGGCCTTCAATCTGTC26670.11102674133391947No Hit
CCTGGCCTCCTCTTGATGGCACAGGACCTTCCCCATCCCGCCCCTGGAGC26500.11031903432129231No Hit
GGAAAATGCTGGCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGC26390.10986110625429828No Hit
GCCATGGTGGAAATGATGTCTGTGCTGCCACTGTCTCTCTGTGGTAATAG26350.10969458695720953No Hit
CTGTCCTAATTCCCCACTCCTCTAGGCAGGGCCAGCATTTCTGCGCGGGG26160.108903620296038No Hit
AATGCCAACTACCAGGTGTACGACAGCCTGGAGCTGAAGCGGCAGGTGCA26030.10836243258049959No Hit
GCCATCCCAGCTTTGCTTCTGTGCCTCCCGCTTCTGTTCCTGCTGTTCGG25980.10815428345913866No Hit
GGCTCAGCAGAAGAGAATAGTAGTAGTGGTGAGCAGCCCTCGTTCGCCAT25920.10790450451350554No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATCACCTTGATCATTT25800.10740494662223933No Hit
CGGATGAGAAAGGAGTCGATGAAGTCCCGTGGGGAATTGGGATCCAGCGT25770.10728005714942276No Hit
GATTTCCACCACAACAAGCAACGCCACCTTTGATCATGGAAGAAAGACCC25610.10661397996106778No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCGTTACCTTGATCATTT25240.10507367646299692No Hit
GGTGATAGCAGTTGGTGTCATGGAGCTCAGGAATCAGAGCCTTGGTTACA25190.10486552734163598No Hit
GGGCTAGGCTATAAGTGACCTGACCATTGGTGCCTTGGTCAGGATCCCGA25180.10482389751736379No Hit
GGTTAGGTAGGAGCTTACTCAGTGCATCTGGAGGATGGAAAACCAATGGC25130.10461574839600288No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTACGATGTGTA25100.10449085892318631No Hit
GGAACCAGTAACCAAGACCAAGCCGTGGAGGGAGCCACTGTGGGAGCGGA24980.10399130103192007No Hit
GTCCGGCGATAATAAGCAAACGCTTCTGCTTCTTTCTGTAGTTTGTCACT24970.10394967120764789No Hit
ACCCATTTTGTGACAGATGCTGCCTCTCCCTCAGGCCCTTCACCTTCGTG24970.10394967120764789No Hit
CAATGCACCTGCCTTTGACCGCCAGCTCTACCGACCTGAGCCCCTGCCTG24940.10382478173483134No Hit
ACCAACCCTACAGCTGAGATCCCACCGGACCTACAACGGCAGCTAGGACA24830.1033668536678373No Hit
GGATGAGGATGAGGACATGACCAAGACCCGGACCTTCTTCGCAGCCAAGA24620.1024926273581214No Hit
CCAACCTGAACCGTGTGATTTATTTCATCTGTGACACCATCCTCTGGGTG24500.10199306946685517No Hit
GGCCTTAATCTCAGTGCTCTTCCTCCTCGCCGTGATTCTGGCCATTGCCT24500.10199306946685517No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC24490.10195143964258299No Hit
GCTGACCTCAACAAGTGGATCAGCGCCATGGAGGACCAGCTGCGGTCAGA24490.10195143964258299No Hit
CAGTAAATTACATTTAAGAAATTCTGAGCATTATGGGGGTGTATAGATCG24400.1015767712241333No Hit
GACATTACCACAGGACAATTTGGCTACAGACATGTGGTCACAGTAGGTAT24310.10120210280568365No Hit
CTGATGCATGACAGCCTGAGGCCACACCCCCCTGTTTCTGCAGGCCAGGG24250.10095232386005053No Hit
CAATGGGATAGTGACGAAATGGAACAAGAGGAAGACATCCTGCTTCTACA24240.10091069403577835No Hit
GACCAACTTGGCCCATCTCTCCCTGCCTTCTGAGGAGCTGGCATCCTGGG24170.10061928526587305No Hit
AGCTTCTCCACAGTGTAGACTGAAACAAGGGAAACATGAACATCACAAAC24150.10053602561732866No Hit
CAGCATGATTAACTGCACCAGGGAGGTGTCCACACAAACCAATTTCAAGA24140.10049439579305648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)