FastQCFastQC Report
Wed 8 May 2024
EGAF00001129762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434308
Total Bases365.1 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTCCTTCCATGGTGACTCTGGCTCTTGCAGGAGGTGGGCTACTGCAG124420.51111034429497No Hit
AGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTATCATTATC100130.4113283939419334No Hit
AGTGTGCCTTGGCTGAAAGCTGTGATCACCTGGCCAGGGTGGCCATCCCC83580.34334192715137113No Hit
GGCATACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCC71380.29322501507615306No Hit
CAGACCCATGCTGACCATAGCTGGAAGATGAACCTGAGCTAGATCCGTGT57620.23669971096508738No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGGA57350.23559056618965227No Hit
GCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGTAATGAAGCAGGGGA50140.20597229274192091No Hit
GGTTTGAAGACCACTTAAAAGCTTTGCCACATTCTTGACATTTGTAGGGT47080.19340198528698915No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAAC46630.1915534106612639No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATGCGTAGTAAACTTACAT44240.18173542542685642No Hit
GAATGGCTTGGATGGTGAGATCAGAAACAAAATTAAGTGAGATCGGAAGA42870.1761075426774262No Hit
CAGACCCATGCTGACCATAGCGGGAAGACGAACCTGAGCTAGATCCGTGT40450.16616631913463703No Hit
TTGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGT40060.16456422112567515No Hit
GCCCACAGCATCCTTACCTGCTGTGTGACTCGTTCTGGGTACCATCTCCC39860.16374263240313058No Hit
TTACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAAC38510.1581969085259548No Hit
GGATACTCATTCTACCCAGTGTCACGAGAAATGAAACAGGACCCTATCAA37690.15482839476352211No Hit
AAAAGGTTAACTTGAGGTTGATTGTGCTTGAGGTTGGAGATCGGAAGAGC37190.1527744229571607No Hit
CTGCTGTCCAGACCATATTGACTTTCCATTTGCCCTACTGTGGACCCAAC36740.15092584833143546No Hit
GAAATATCCAGTTACTCCTCTAGTCCCATCACCTCGCTTTATGATGTGTA35840.14722869907998495No Hit
AGTAAATACATTGGTTGAAGTTTAAAAGTCTATCCGTAGTAAACTTACAT35160.14443529742333344No Hit
CACTTAATTTTGTTTCTGATCTCACCATCCAAGCCATTCAGATCGGAAGA34850.14316183490338938No Hit
ACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCC34600.14213484900020867No Hit
CCAACCTCAAGCACAATCAACCTCAAGTTAACCTTTTAGATCGGAAGAGC34580.1420526901279542No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTT34260.14073814817188293No Hit
GGGATGAGTGGAAGGTTCTCTTACGGAGTTACTCGTCATCTTCCTCAGGG34110.1401219566299745No Hit
TACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCCAGCTACGAACAACA34050.13987548001321115No Hit
GTAGGTGTCTTCTCCTCGATCCCTGGGATGGGAGCCTTGTGGGGTAGTAT33920.1393414473435572No Hit
CAGTAAATTACATTTAAGAAATTCTGAGCATTATGGGGGTGTATAGATCG33890.1392182090351755No Hit
CATCATGATCAACCAGTGAAGCCCCTGGATCGAGCAGTCTTCTGGATTGA33730.13856093805713984No Hit
GACCTGCGCTGTGAACTGCCCAAGCTGGAGAAGCGGCTGCGTGCCACGGC33170.13626048963401508No Hit
GATCCACCTCCTGCTGGTAGCGCTTACGGTCCCGCATGGCGTTCTCCTTG33120.13605509245337893No Hit
ACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGT32860.134987027114071No Hit
CTCCATCATAGGACCCAAAAGGGGGGAACATGCCTGGACCCACAGACTGC31850.130838004065221No Hit
GGTTGATATGGGAGTAAAGACCTTGGCATGGTACATACCCCTCATGCAAG31570.12968777985365862No Hit
TGTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTT31210.12820892015307842No Hit
AGGTGACACCATGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAG30910.12697653706926157No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCAATACCTTGATCATTTC30670.1259906306022081No Hit
GTATTTCATTATGTCCTGAGGAACAGTTGGTTATAGAAAGGCTACAAGGA30450.12508688300740908No Hit
ATACACCCCCATAATGCTCAGAATTTCTTAAATGTAATTTACTGAGATCG30310.12451177090162789No Hit
GGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCAGGTGAGGTCGTGG29200.11995195349150559No Hit
ACCACGTGAAGGTGATTGCTGGCCGATTCGAGGGCGACACAGGCCTCATT28770.1181855377380348No Hit
GAAGGCTGTGTACATGACCTCCTTGGACACGGAGATCGGAAGAGCGTCGT28580.11740502845161746No Hit
TACAGTCAGCATGGATCTGGCTCAGGTCACTCCTCTGGCTACGGACAACA28560.11732286957936301No Hit
CTCAGTCCATTGCCCCAAGGCTCACTTGTTAGCTTGCCTGGGGAAAGGGG28330.11637804254843677No Hit
TGGAGCCAGACCATAAATACAAGATGAACCTGTACGGCTTCCACGGTGGC28230.11596724818716449No Hit
CCTTCCCCGACCCTTCTATCCACCTACCTCATGCATGGTGAGGTCAGAGA27930.11473486510334766No Hit
GTGTGAATGCAGTCATCTGTATAAAATGTCATCATTACCTTCATCATTTC27630.11350248201953081No Hit
GTCAAAGGAGTGTTGGAGTCAACAGGAGTTGTTGAAAGGGTGCTAGCCTC26970.11079123923513376No Hit
GTCCGGCGATAATAAGCAAACTAGAAGTATAAGGTGACAAGATCGGAAGA26900.11050368318224317No Hit
GGAGAGCCAGCCATTGTGGGGGCAGCTCCACAGATAGGACTCATGTCCTG26400.10844971137588177No Hit
GGAGGAAGAAGATCTGCACACCACAGCCAGCGCGGGAGATGGCCTTATTT26250.10783351983397335No Hit
GGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCA26240.10779244039784612No Hit
CGGAGCATGTAGGTGTAGAAGGAAGCACCGAAGAGCAGCAGCACTATGAT26100.10721732829206494No Hit
AAGGCACACTTACCACTGTTTTCTTGGCTCCAGTCCAAACCTGAGAGTAG25980.10672437505853818No Hit
GAACACCCCACACATTAGGGCGGCTGCCTTGTCCTTCTGGGAGGAGCTGG25810.10602602464437531No Hit
GACAAGCTGGTGTCTGTATTCTACACCATCCTCACTCCCATGCTGAATCC25700.1055741508469758No Hit
CCTGCATATAGTGGACGAGAAACAGCATATTCCAATGCATCCCTGCTGAT25430.10446500607154065No Hit
TGCCTCCTTGGTATGAAACATCTTTCACACAACCCTCAATTGTAGGAGTC25220.10360233791286888No Hit
GCCACCAAATCGGGAGAAGCGGCGGTCTCCGCTGAAGGTGCTGAGCAGCA25110.10315046411546937No Hit
GCCATCATTTCCTGGGAGAGATGGATGGTGGTCTGCAAGCCCTTTGGCAA25060.10294506693483323No Hit
TGTCACCTTATACTTCTAGTTTGCTTATTATCGCCGGACAGATCGGAAGA24880.10220563708454314No Hit
AAAGTACTCACTCTCCACACTTGCCCTTTCCTAACACTTTCCTGAGTGCT24540.10080893625621737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)