FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001129766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707451
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC108740.40163238411332286No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC72870.26914614521186164No Hit
AAGCATTCAAGTAGCAGGTATTTCCCATATTCTGGAGCCCAGCCCCCACC72490.267742611038944No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG71610.2644923213753453No Hit
CTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGC66430.24535993449188923No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA61650.227704952000978No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT58310.21536862532322837No Hit
GAGAACTCCTTTGTCCATTTGCACGTATGGATCTTTCCTCACTGCTTGAT52650.1944633531687185No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA52060.19228418168971478No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC51280.1894032431242523No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC48000.17728852710538437No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA45840.16931054338564208No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG44910.16587557817297524No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC44890.165801707953348No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG44630.16484139509819384No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC42710.15774985401397845No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC42370.15649406028031532No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT42350.15642019006068808No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA36180.13363122730568347No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG35790.13219075802295222No Hit
AGGGTCACAGATCTTTAACAAGAATGGGGGCACTCAGGGTCTGAGGCCAC34420.12713064797848603No Hit
GGGCTGGACATCTTGAAGTGCCGCACCACAAAGTCCTTGCCGCCCATCAT34060.12580098402519566No Hit
CACACTCGTGCAAGGCTTAGAAGGCCAGAAGATCACACGCGTGGCTTGTG33840.12498841160929598No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA32740.12092554952979759No Hit
GTGTGATCTTCTGGCCTTCTAAGCCTTGCACGAGTGTGGGCTTCCTGTTA31860.11767525986619888No Hit
GCACAGGCTATGAGAAGTACAACTCCATGCGAGCTGACCCCGCGCTGTGC31120.11494206173999087No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG30890.11409255421427757No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC30550.11283676048061443No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA30130.11128548586844232No Hit
GGAACCCCAGAAAAGACCTTGCCCCTTCTGTCCCTGCAGATGACGTCCAC30060.11102694009974695No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT29450.10877389840111604No Hit
GAGGATGAGGAGGAGATGAACGCGGAGCTCACTGCCAAGAAGCGCAAGCT29360.10844148241279344No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG29190.10781358554596188No Hit
CTCTGCTCAACCTGTCTTGGCCCGAGGTGGATGAGGGTGCTGTCCTGGGC29020.10718568867913028No Hit
TTCATGGAACGGTATAACTGGAACCGACGCTTACGGAGCAAGGGTATGCG28730.10611457049453527No Hit
GTGAAGACCAAGAACATGAAGATGATGGTGAAGATGAAGATGATGAAGAT28370.1047849065412449No Hit
GGGATCTCCGATTTCTCCTCTGCTAAGGTCAGAGGTACTGGTGCGTAGGC27980.10334443725851362No Hit
GCTGGTTGCCTCCTTGTGGGGGTGGTCCTTGTGGCTTTCCTGGAGGAGAT27970.10330750214870002No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGATGCCTGGGGCTGGCCATT27520.10164542220708703No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT27270.10072204446174648No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG27250.10064817424211925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)