FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001129789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224529
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCACTGCCAAGTTAGACAGCCCCAGGGTGACAGTGGCCCAGCTGGAC162990.5054691708463468No Hit
GCCGTAAAACCTCCAGTGACCCGTTTGCCTTCCCTCCTTCCCCCAGGTGA72990.2263586402851393No Hit
GGGAGCGTCACGTCTACCCGCCAGTTCCAGTCATAGCTGACTTGCAGTCC62190.19286537661779443No Hit
TGGTGATGGGTGTCGATGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTT62010.192307155556672No Hit
GGTCTCTTGCAGTCTGGAAGAGGACGGGCTCGTGGACAGAGGGCGTGGCC56480.17515736406774446No Hit
GACTCTTATCCATCCGTCCTCTCCCAGCTCACCAATCACTCGGCCTAGGC55310.17152892717044876No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGGTGCCCGGGGCTGGGCATT55060.1707536201411121No Hit
CCATGGCTATCCCCATCTGGGTCTGGCAGAAGTTCTCCTCCAGTTGTGCA51740.16045754279152086No Hit
CTTGTCTCCCCAGATGTCAAGAATATGAACACGTGGCTGCTGTTCCTCCC51250.15893794101402098No Hit
GCATGCACCCTCCTAACTGAGGTCTGGTGGGAGCCATGGAAGGACCAGGG49830.15453419708738858No Hit
CCTGATTCCTGTCCTCAGCTACCTGGCCAATGACATGGAGGAGGACGACG48930.1517430917817765No Hit
GCCCCACCAAGCCAGATAGGAAGGCCACTGTTGGGAGGCAGGAGGGAAAG47490.14727732329279716No Hit
GGTGAAGCAGTGTCCTTGTCCCAGGGGCTGCTGCTGTTGCTGCTGCTGAG47470.14721529873045025No Hit
GGAGGATTCTGTGGCGTATGAAGACCACGGGATAACCGTATTGCCCAAGA47250.14653302854463396No Hit
CGATATCCCACTCTCATGAGCTGGCAGCTGTGCCTGAGGATGACCATGTC44250.13722934419259372No Hit
CAGAGCCTGGAAATGGTTGAGACTACCCTGCCACGATTTTGCTCCCGCTC43980.1363920126009101No Hit
TCCTTGGGTCCATCGTGTAGCACCAGGGCCCATGGCTATCCCCATCTGGG43430.13468633713636938No Hit
CTCGCAGAAGAAGTGATCGATCTCGTGTGCACCGCAATATGGGAAGCTCA43320.13434520204346123No Hit
GCACCAAGCCAAGAAAGTCCAGCAGGAGCACGAGCATCCCGATGATCACG43140.13378698098233882No Hit
ACCTGTCTGTCCTTTGTGTCCAGGAATTCCCCAGACAGTGGGGAGGGAGG40350.12513455453494138No Hit
CTGGTATCTTGTCCTGTTCCTCCTTTTCCCTTTCCAGAGGCCAAGAAGAT39640.1229326825716252No Hit
CCTCTCCAGATCCCATGTGAATGACAGCCAGAATAACTCGAGCATAGGAA38380.11902513514376828No Hit
GGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGC38170.11837387723912546No Hit
AGAGCCCACACGATGAAGACCCCCAGGCAGTGACGTATGCCGAGGTGAAA38090.11812577898973774No Hit
GAGATTGGAGAAATAATATGCCATGGTATTACATAAGCTTTGGCTTCTCT37520.11635807896285008No Hit
GATATGCTTTATGGTCTGCTCAGTCACCTCGTTCTTAATCCCTTTCTCAA37200.11536568596529913No Hit
GTGTCCAACAGGAGATCGACGACGTGATAGGGCAGGTGCGGCGACCAGAG36570.11341191225137066No Hit
CTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGGTGTCACCT35810.11105497888218713No Hit
CAGATGAGATCAAATTAACTCTAGTGTGCTCTGGCAGAGCCTTACCTCTC35100.10885310691887094No Hit
AAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGATGATCTCAA35020.1086050086694832No Hit
CGATGACAGCGCCTTCATGGAGCCGAGGTACCACGTCCGTCGAGAAGATC34740.10773666479662611No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACA34720.10767464023427918No Hit
CACAGATGGTGGTGTTGACGGTGATGCACACGGGGCAGCCCTCCTTCTCC34270.10627908758147314No Hit
GGTAGAGCTGCAAGGACACCACCTGCACAGGGTGCCCGGGGCTGGGCATT34160.105937952488565No Hit
CCACTACGGTGACACCAACCCCAACACCCACCGGCACACAGACCCCAACC34140.10587592792621807No Hit
GGCTCACTCACCAGAGATGCGGCCTGCCACGTGCACTCCCATGGTGACTC34110.10578289108269766No Hit
CTGGAGGACCTGCTACGAAATAACAGAAGACCCAAGAAATGGGTGAGTGG33250.10311583490177946No Hit
AGGCTACTGAGGTGGTCAACCCTCTCCTGGAGCCTGTGGAGCACCGAGAT33240.103084822620606No Hit
GGAAGACTCGGAGCAGGTAGGCACGAAGCACACAGGCTTGTTACAGACGG33190.10292976121473865No Hit
CATGCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCA33180.10289874893356517No Hit
AGGCAACAAACCTCAAGGTCCCCCACCTCCAGGAAAGCCACAAGGACCAC32820.10178230681132036No Hit
GGTAGAGCTGCAAGGACACCGCCTGCACAGGATGCCCGGGGCTGGGCATT32470.100696876970249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)