FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001129881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001129881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGTGTGAGAAGCAGGACAGGGTTTCCATGGGAGCAGCAGGGCAGAAA23.389830508474576No Hit
GAACCGGAGCACCAGCTCGAAGGTGAACCACACGATGCAGGTAGACTCCA11.694915254237288No Hit
GGGCCTGTTTATTGTGCTAATTCTGGTGGGGTATATCTCTGTGAAGGTGA11.694915254237288No Hit
ACAAAGTGTTAAGTATTTGAGGTGAATATGTTAAATACATTGATTTAATT11.694915254237288No Hit
TATTCTAAAAATGACAAGAAAGTCTCTTTGGAAACACCATAAACGGGAAT11.694915254237288No Hit
TGTATGTACCTGGGAAATTTGCCGATTTCCTAAGCTGTTGGAAGGGGGTC11.694915254237288No Hit
CCATGGGGCGTGCTGTCTGGAGGTCCAGCTGGCCTTCCCCATGTGACCTG11.694915254237288No Hit
GTGGTGGCTCACGCCTATAATCTCAGCACTTTGGGAGGCCAAGGCGGGTG11.694915254237288No Hit
TCCTTCCAGAGTTGGGAGTTTGGGGCGCTGCTCAAAATCGCCCCCCACCA11.694915254237288No Hit
TCAAAAGAGTAAACACTTAAAAGAAATGTATCAGGAACTAATGGAAATGT11.694915254237288No Hit
TAGCACAAAAATTAAGCTCTTGTGGACCGTAATATATCGAAGGTATGTGT11.694915254237288No Hit
AGGAAGGACATGGTGATGCTATCACTGAATCATAAAACCGTGGGGTGCTT11.694915254237288No Hit
AGCTGCAGGACTCAGCCACAGAGTTAGCGCCTTGCTTACATGTCTTTTTC11.694915254237288No Hit
TTATGTCATCCCCATTTTAGTAGTGATCATAATAGCTAACTAGCATTTAC11.694915254237288No Hit
TTTGAGCTCTGAGAGACTTGGCTAGATGGAGGAGGGGAAGAAGATGCTCT11.694915254237288No Hit
TGCATGGACTGCCACTCCTCCCCTCCTCTCTGCATCCTGGCTTTCCCAGC11.694915254237288No Hit
CTCAGCCTCCCAAGTAGCTGAGATTACAGGCGTGCACCATCACGCCTGGC11.694915254237288No Hit
CTCTTTCTTGAAGGTCTCCACGAAGCCATCGAGGCTCTCCTTGCGGCCGT11.694915254237288No Hit
CAGCGAATGTCCACACACGTGGTGAAGATGTCTGCGAGGTACCGTTGCCC11.694915254237288No Hit
TGTTTGTATTACAGTGCTGTCCATGCTTCCAACACTGAGAGAGGCCTTAA11.694915254237288No Hit
TCCTCAGTTCAGTAGGAAGGAACAGAGCCCACAGCCAGGGCTACCAGAAG11.694915254237288No Hit
GAGGTTATGGGTGGGGAAAGATACGAAATACAGGAGACAGGAGAGGGAAG11.694915254237288No Hit
TGCGTTGTTTCTCCTTTGTATGTTTGACAACGTCTGTGATTAAAAATAGC11.694915254237288No Hit
AGCTGCAGGACTCAGCCACAGAGCTAGCGCCTTGCTTACATGTCTTTTTC11.694915254237288No Hit
ACAAGGTTAAATACAAAGGATTAAGCACTGAACTGCTTTATTTTAGGTTC11.694915254237288No Hit
TGAACTTTTCCTATTGTGGTGGTGTATTGAATTTAAGCCTTCTATCTGAA11.694915254237288No Hit
GAAGGGGTAACTGCGAGGTGTAATTAGAGACTTCGAGGGGACTGGAACCG11.694915254237288No Hit
GTGAACACAGTTTCTATTAACTAGTTGCTACTTCTTACCTGAAAGTATTC11.694915254237288No Hit
GCATGGAGTGTAAACATTTTCCCCCGCTGTTCCCTGCTCTGAGAGCATGA11.694915254237288No Hit
GCAGCGGCTTCGAGGAGCTGGTACGTTGCCCCACCTGGGGAGGCTGCCCT11.694915254237288No Hit
TTTCCTTAGCCCTGCTCCTCTACTCACCTGGCTCCTGGACCTGGCTGTGT11.694915254237288No Hit
CGCCCCCACCCCTCGCGCCAAGAGTGCGCAGGCGCGCAGCCAGCTCCCCC11.694915254237288No Hit
GGGGAGAACCCTTCCCACGTCCCCCACCCGGCCACCACCTTCTTGGTAGT11.694915254237288No Hit
GAGCGTGTAGATGCAGGTAAGTCCGTAGATGCACACAAAGGAGATGTAAC11.694915254237288No Hit
AAATTTTGTTTAATTTGAAAAATTTAAAACTTAGAAAGATACTGTGTTGG11.694915254237288No Hit
GTTTTCTTTTCTTTTTTTTCTTTTTATGGTCACTTTGGTAACCACTGGAG11.694915254237288No Hit
AACCACCATCAACTTTAGGAGGAGGGAAAGGGGTGGCTCTGGCAGGAAAA11.694915254237288No Hit
TGAACATTTCTCTCTGATGATGGACAAGACTAAAGGCAAGAACGCTCCAG11.694915254237288No Hit
CCAGGGGACAATGTGGGCGCCAACTTCGCCACCAGCCGGGTCCAGCAGCC11.694915254237288No Hit
ACCCACAGAAAGCCGGGTCTGGCACCCATAAACCACTCAGCTGTCATGAA11.694915254237288No Hit
CTTCCTTCAGTCCCTCTGCCTCCCTTTGCCCAGGCCTTTCTCAGACTGTA11.694915254237288No Hit
CACCCCCTTCCTGCTCTCAGCAGGCGCTCAGCTTACCTCACCCCAGGGCC11.694915254237288No Hit
GCCAGGGTCCTTGAATGGTCTCGTGGCACAGATCCTGCTGTGGGCAGTTA11.694915254237288No Hit
AGTGATGGCAAATCTCAGCCCAACAAGGGGCACAAGGCCCAAAGATTATG11.694915254237288No Hit
AAAGGACAATACTGCTTCCCCCCCCACCCCCCGAGGATTTTGTTAAGGAA11.694915254237288No Hit
GTTCAGCACCTGGCGCACAGGGCTTTGGTTGCAGATGGTGATCTGACGGG11.694915254237288No Hit
CTTCTCCACCAGGCCCCCGTTGGCCTCCGTCTCCACCGAAAGCCAGTCAT11.694915254237288No Hit
GAATCCATCCAAGTTATTTAGAAAGGGAGCGTCAGATCAACCTTTCCAAA11.694915254237288No Hit
AGCACATCCAGAGGTAAAGAACCTAAAATCCATTGAGTTAGCATTCTTTC11.694915254237288No Hit
AGCTTATGCAAGTTTTGCAACATCTCTGCATCCAAATGTCCTTGTTTTCA11.694915254237288No Hit
CTCTACAGGATTTGGGAGACATCGTGGCAAGGTATGTTTTTGGCCACCAG11.694915254237288No Hit
TGCAATCCAACAGCAACAACTGGCGCTGGTTTGATGATCGCTCTGGGCGT11.694915254237288No Hit
TGGTCCTCCAGTGGTGCGCTTGGTTGTGACTGGTCCCTCTCACAGGCCTG11.694915254237288No Hit
CCGCATGGTGCGTCGATCCCACACTTTGCAGATGGCATCATCTCCCCCAG11.694915254237288No Hit
CGCTGACCGGCGACACACTTCGTAGCGGGACAGCTGCTCCTCAGACATGG11.694915254237288No Hit
CCCCGCCCGGGGTCCAGGGTTTGAGATGGCCCTCAGCCCCTGACTTGCTG11.694915254237288No Hit
TGGCCACACTGCCTCCACTCTGCTGAGTGCTCGCCTCTGCACAGAGTCTC11.694915254237288No Hit
CCTGCCTGTTTGGGACCCTGACTGTTTTAATGTTGTCTCAGGTGCTCTGC11.694915254237288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)