FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392848
Sequences flagged as poor quality0
Sequence length44-300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT5117313.026157699670101No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC4233110.775414409644442No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG4117010.479880259031484No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT317398.079206206980817No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC294877.505956502260417No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG85182.168268643342972No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA74381.8933531544006843No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT51691.3157760762432291No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC45151.1492994746059546No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA34860.88736610597483No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG32860.8364558302447765No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG26580.676597564452409No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA23230.5913228526045697No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT22400.5701950881765975No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGA21950.5587402761373356No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG18510.47117460188164384No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT18460.46990184498839244No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC17970.4574288274345294No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGG17030.43350099784140433No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATCT15590.39684559931576585No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC15500.3945546369079135No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAT14630.37240866696534025No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGTAATCGAAAT14590.37139046145073923No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAA14220.36197206044067937No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATCC13740.34975359426546654No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAGGGT13100.33346230603184945No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAA12690.32302569950718857No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCGAAACG12550.3194619802060848No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCG12010.3057162057589704No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGACATCAAAAG11970.3046980002443693No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGGGGTCGAACC11120.28306113305909664No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCAT11120.28306113305909664No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACCACGGGG11100.2825520303017961No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTAA11070.2817883761658453No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCATA10900.27746100272879076No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTGTCC10660.2713517696411844No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCCACTGCTACCCA10400.26473343379627745No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGG10400.26473343379627745No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGTGGG10310.262442471388425No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATTCTATAG10150.25836964933002077No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACGTGGGGA9980.25404227589296624No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGTGGACGGAC9950.25327862175701543No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGTAA9940.25302407037836516No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAGCAC9830.2502240052132122No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATTCC9780.2489512483199609No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACATA9770.24869669694131064No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCACCAGCCAC9570.2436056693683053No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGT9560.24335111798965506No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGGT9540.2428420152323545No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATAC9510.24207836109640368No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAAGTAG9440.24029650144585182No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCC9440.24029650144585182No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCG9390.2390237445526005No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG9380.23876919317395023No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTGGAATATCG9330.2374964362806989No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAGTAT9250.23546002525149679No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC9090.23138720319309247No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGC9030.22985989492119088No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCCCGAC8760.2229870076976337No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCGGGGAG8680.22095059666843156No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGCCAG8660.220441493911131No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATCCGGACG8580.21840508288192886No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT8410.21407770944487434No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGGAGT8380.21331405530892353No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTTTCC8370.2130595039302733No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCGCTGCGGTA8150.2074593735999674No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGCTCGAACGGG8010.2038956542988637No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGA7970.20287744878426262No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTCAAAGA7950.20236834602696208No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGAAAT7850.19982283224045944No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCAGTCAAAGT7840.19956828086180917No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGAGCCC7710.19625911293935566No Hit
CTTACCTGAGGAGACGGTGACCAGGATTCCCTGGCCCCAGTAGTCAAGAC7590.19320449639555248No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCATTGT7540.19193173950230116No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAATAAC7500.19091353398770008No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGGGGG7370.1876043660652466No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGGCT7200.18327699262819208No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACTAT7190.1830224412495418No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCACCCACTGT7130.18149513297764022No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCC7120.18124058159898992No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTTCTTGTAC7090.18047692746303914No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTGACCGA7050.1794587219484381No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTTCCGAGAC6940.17665865678328513No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCTACTT6840.17411314299678246No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACGCC6810.17334948886083165No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTTCCC6800.1730949374821814No Hit
CTTACCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGGAGTCAAAGT6790.17284038610353114No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGT6780.17258583472488087No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGAAG6520.16596749887997395No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTTGCCCCAGACGTCCATGT6450.16418563922942206No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCGCTA6390.16265833095752047No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACG6390.16265833095752047No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCAATTATAA6320.16087647130696858No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGACGTACATAG6310.16062191992831834No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCGAGG6150.15654909786991408No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACCT6070.15451268684071193No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGGAAATCG6020.1532399299474606No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGTACTCGCT5890.14993076202500713No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAACC5870.1494216592677066No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTTCCAAGTA5750.14636704272390338No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCTTTCCC5720.1456033885879526No Hit
CTTACCTGAGGAGACGGTGACCTCCCCAGTCAGGGTGAGTAGTGGTGGGG5670.14433063169470126No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT5530.14076691239359754No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAATCGTAAC5460.13898505274304565No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC5430.13822139860709487No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCACCG5320.1354213334419419No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGCCTC5090.1295666517329858No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC5050.1285484462183847No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCCAGCGGGCGGGT5020.12778479208243393No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAACCAC5020.12778479208243393No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG4720.12014825072292591No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCACAT4700.11963914796562537No Hit
CTTACCTGAGGAGACGGTGACCAATGTCCCCTGGCCCCAGATATCAAAAC4620.11760273693642324No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTGTACAACG4530.11531177452857086No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTATACCCCG4340.11047529833421578No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCAC4330.1102207469555655No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCTTACTAAC4240.10792978454771311No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCGGCTGCTATAGTCAC4170.10614792489716124No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAGCTC4110.10462061662525964No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGCT3950.10054779456685538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)