FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001132162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68104
Sequences flagged as poor quality0
Sequence length45-300
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC963714.150417009279925No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG924313.571890050510982No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT788811.582285915658405No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC38825.700105720662516No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATTA25493.742805121578762No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC25403.7295900387642433No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG19122.807470926817808No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGTAGC18962.7839774462586635No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCGG17452.562257723481734No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT16952.488840596734406No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGGACG16042.35522142605427No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTTTCCCCGCC13041.9147186655703043No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG13031.913250323035358No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCACCTC12521.8383648537530834No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCAGGCACTGCTATACCCGGGCC12101.7766944672853284No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCCAGCCCTT11821.7355808763068248No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCGCGG9561.403735463408904No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG6590.967637730529778No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGT6460.9485492775754728No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACA4670.68571596382004No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT4630.6798425936802537No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAAG4410.6475390579114295No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT4290.629918947492071No Hit
CTTACCTGAGGAGACGGTGACCCGCTTGCAGTATCTCCCAGTTTCACCAC3960.5814636438388348No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATCCCCAAG2830.4155409373898743No Hit
CTTACCTGAGGAGACGGTGACCCCAGGCTGTCCTGCAGGACCAGGAACAA2630.3861740866909433No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT2600.38176905908610365No Hit
CTTACCTGAGGAGACGGTGACCAGCCCAGGCAATGTAGCTGGTAAACGGC2060.3024785621989898No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCCTGGCCCCAGATATCAAAAG1410.20703629742746388No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC1410.20703629742746388No Hit
CTTACCTGAGGAGACGGTGACCCAAGAGGGAGCAGGGTGACACCCACTGG1200.17620110419358628No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCGGAGT1150.1688593915188535No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC990.1453659109597087No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTAGAAGC750.11012569012099144No Hit
CTTACCTGAGGAGACGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGG720.10572066251615175No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC690.10131563491131211No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC690.10131563491131211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)