FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492017
Sequences flagged as poor quality0
Sequence length79-300
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC35946973.06028043746457No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC5236710.643331429605073No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC45140.9174479743586095No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC32250.6554651566917403No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGC29090.5912397335864411No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT23650.48067444824060956No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGC23170.4709186877689186No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC21830.44368385645211444No Hit
CTTACCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGTAGTCAAAGC17560.35689823725602976No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCGTCAAAGC17080.3471424767843388No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG16700.3394191664109167No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTTGCCCCAGTAGTCAAAGC13420.27275480318769474No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAACC12490.2538530172737934No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCAAACC8430.17133554328407352No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC6620.1345481965054053No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGC6530.13271899141696325No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGAAGC6190.12580866108284877No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC6140.12479243603371427No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG5640.11463018554236948No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGTAGTCAAAGC5580.1134107154834081No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC5300.107719855208255No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCAAGC4940.10040303485448673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)