FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001132167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621163
Sequences flagged as poor quality0
Sequence length35-300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAC10947417.62403749096453No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACC6923211.145544728195336No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGT402856.485415261372619No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC374586.030301225282253No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAG317805.116209432950771No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT192873.104982106146052No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGG86161.3870755341190637No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGT70061.1278843073396194No Hit
CTTACCTGAGGAGACGGTGACCACAGGAGACTGTGACAGTGACGGTGACC69081.1121074500573924No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGA49070.7899697824886543No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAG44780.7209057847940074No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATA37210.5990376117057841No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATAC35170.5661959904244136No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT34640.5576636084248418No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACC32440.5222461737096382No Hit
CTTACCTGAGGAGACGGTGACCAGGGTGCCCTGGCCCCAGTGCTGGAAGT28940.4659002548445417No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAC22540.36286771749122215No Hit
CTTACCTGAGGAGACGGTGACCACAGACAGGCCACCCAGGGCTCGGGCTG21850.35175952205781735No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAG19560.3148931922860827No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGG19250.30990255375803133No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATC18940.3049119152299799No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA17640.2839834310800869No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATG17510.28189058266509753No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAATT17140.2759340140993588No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAC16010.25774233172291333No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAT15360.24727808964796677No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCTTGGCCCCAGACGTCCATAC15200.2447022762141338No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACT14640.23568692919571835No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGC12470.20075245949935844No Hit
CTTACCTGAGGAGACGGTGACCCACACCAAGCTCAGAGAGGGCAGGGGTG12370.19914257610321287No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGT12210.19656676266937984No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATGC11210.1804679287079237No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGTT10950.1762822318779451No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACA10480.16871577991606068No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACG9770.1572856078034268No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAGCC9390.15116805089807345No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTTGCCCCAGACGTCCATGT9180.14778729576616767No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGT8870.14279665723811624No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAAGC8700.1400598554646687No Hit
CTTACCTGAGGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAC8480.13651811199314834No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCGTACC8470.13635712365353378No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGTTAACCACT8430.1357131702950755No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCATGGCCCCAGACGTCCATAT8060.12975660172933676No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCCTGGCCCCAGACGTCCATAC8060.12975660172933676No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCC8020.1291126483708785No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGACCC7840.1262148582578164No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGC7570.12186817308822322No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCCCCA7350.11832642961670287No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCTATGG7340.1181654412770883No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCATTCC7260.1168775345601718No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCCGAAT7220.11623358120171357No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAAC7210.116072592862099No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAACT7030.11317480274903688No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAA6970.11220887271134951No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCCATCC6950.11188689603212039No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACA6880.11075997765481846No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTGTGCCCCAGACGTCCATGT6760.10882811757944372No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCGTGGCCCCAGTAGTCAAAAC6690.10770119920214179No Hit
CTTACCTGAGGAGACGGTGACCAGGCTTCCCTGGCCCCAGTTGTCAAAGT6640.10689625750406899No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATAGC6600.10625230414561074No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAAGT6600.10625230414561074No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCGAACC6600.10625230414561074No Hit
CTTACCTGAGGAGACGGTGACCAGTGTCCCTTGGCCCCAGATTTCAAAAG6540.10528637410792335No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCATCGG6530.1051253857683088No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAAC6390.10287154901370493No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGGT6350.10222759565524668No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGT6330.10190561897601756No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCGGTAGACCCCTCT6280.10110067727794475No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAAGC6250.10061771225910107No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTATCCTAAAA6250.10061771225910107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)