FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384349
Sequences flagged as poor quality0
Sequence length300
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACA25181165.51623654543135No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACC70701.8394740197060486No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC55821.452325880905115No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA19110.49720436374232796No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGCACTACTACA16800.4371027373558927No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTCCA15250.3967748062307955No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGCTGGTACTACTACA11720.3049311953458966No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC9110.23702416293524894No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG8680.22583641430054457No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTAATACA8050.20944506164969856No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGCACTACTACC7890.2052821784367853No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTCCTACA7570.19695641201095881No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTAAA7330.19071208719158889No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCACGATGGTACTACTACA7150.18602884357706148No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTATA6120.15923028289393232No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGTTACTACTACA6060.15766920168908985No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCCTGATGGTACTACTACA6020.15662848088586157No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTTCTACA5750.14960361546407042No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGACGGTACTACTACA5740.14934343526326335No Hit
AGGGTTCCCTGGCCCCAGGGGTCGATCCACCCCATGATGGTACTACTACA5700.148302714460035No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACTACT5270.13711496582533061No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTACCACA5030.13087064100596074No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCATGATGGTACTATTACA4740.12332541518255544No Hit
AGGGTTCCCTGGCCCCAGGGGTCGCACCACCCCATGATGGTACTACTACA4410.11473946855592182No Hit
CAGGAAGGGGCTCTGGTGCCGGGCTGAGCGGGGGAAGCAGGGGTAGCGGA4340.11291820715027229No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG4070.10589334172848115No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAAGGTGTAGCTCCAAAAAT3990.10381190012202451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)