FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404088
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGACGAGCTGCTATT18384745.49677298014294No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTAAAGCA16021439.64829443091604No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGCCCAACCACTCCAAAA92762.2955395854368352No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGTAAAGCAGCT16550.40956425333095764No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGTAGTAGTAGGACGAGCTGCTATT15010.37145374274910414No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTAAAGCA11950.2957276632812655No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTCGTAGTAGGACGAGCTGCTATT8530.21109263328779868No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTACGAGCTGCTATT5960.14749262536873156No Hit
ACAGGTCTGCCGCCTTGCAATGCGACGCCTGCCTGGGAGCGCAGGTGAGG5790.14328562095385164No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGCAGTAGTAGGACGAGCTGCTATT5330.13190196194888243No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGACGCGCTGCTATT4910.12150818633564965No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTAACGCA4630.11457900259349448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)