FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403102
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTAAAGCA4157410.313518662770218No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGACGAGCTGCTATT399139.90146414555125No Hit
ACAGGTCTGCCGCCTTGCAATGCGACGCCTGCCTGGGAGCGCAGGTGAGG10610.2632088156347525No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGCCCAACCACTCCAAAA7310.18134367976343457No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATGAGCGTAGTTCCAGTT5320.13197652207133678No Hit
ATTGTCCCTCGGCCCCAGATATCAAAAGCGAAAGTAATGGAGCAGGTACC5060.12552654166935417No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAACC4860.12056501828321367No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCTCCGGGCTGGTACTACTAC4790.11882848509806451No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAGTAGTACCCGCCTATGACACTCGC4780.11858040892875749No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTTGATAGC4600.11411503788123106No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCTGCTTCACGATAACTCCC4580.113618885542617No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGCATAAC4580.113618885542617No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGTTGGGGGTTTTGGCCAC4570.11337080937330998No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGACCAGTCAAAATATCGTATGGC4570.11337080937330998No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCGGCAGCTGGTACTACT4530.11237850469608189No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCGAAACC4500.11163427618816081No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCTTGACCACTACTATC4490.1113862000188538No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTACCGACGATAACTCCC4410.10940159066439759No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCCCACTGCTATAATGGGT4390.10890543832578355No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCTGACGTGACTCTCGCACAGTA4350.10791313364855545No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGTAATAA4330.1074169813099414No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCTCCGTACCGGCCACTACTATA4320.10716890514063439No Hit
ACTGTCCCTTGGCCCCACACATCAAGACCATCACTCCTCTCAGGCTCCAG4310.10692082897132736No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGAGA4310.10692082897132736No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGTACCAGC4290.10642467663271331No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCGTAGTAG4290.10642467663271331No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTACCCAAAAGGACCTAC4280.1061766004634063No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGGGTACCAACACAGGGAA4250.10543237195548522No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACGGCGATTGAAATAACTC4240.10518429578617819No Hit
GTGGTCCCTTGGCCCCAGACGTCCATCGCGTAGGAGTAGTAGTAAGAAAC4240.10518429578617819No Hit
AGGGTTCCCTGGCCCCAGTAGTCGCTTTCCTTCTCGTAGGTGGTACTACT4220.10468814344756414No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGAACCAGCTGCTACTATACGCCT4200.1041919911089501No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCGGCTCCAGCTGCT4200.1041919911089501No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGCCTTCTCTCGCACAGTAATA4160.10319968643172199No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAACGGCCCCTCCCACTACT4160.10319968643172199No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCAAAGAAGGTTGCGGACGAACT4150.10295161026241496No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGAAACGTGTA4150.10295161026241496No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCCCGTGTAGGCCAGTCAAAAT4090.10146315324657282No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGTAAAGCAGCT4090.10146315324657282No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTTACCCCGGGT4080.1012150770772658No Hit
GCGGTCCCTTGGCCCCAGACGTCCATAGTGCAGTGGTTACTGCTGACGCC4070.10096700090795878No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGGACGATAAC4070.10096700090795878No Hit
GTGGTCCCTTGGCCCCAGACGCGATACCAGCCACCCGACGCTCTCGCACA4070.10096700090795878No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACTTGTAGGGGTCAGGAGA4040.10022277240003771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)