FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416380
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG34466282.77582977088237No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACATTGTTGCGCCTGATCCTACTAG35510.8528267448004226No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGCCCCTTGG22750.5463759066237571No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAACTTGTTGCGCCTGATCCTACTAG21780.5230798789567223No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGCTCCTACTAG8750.21014457947067583No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTCCTAG7480.17964359479321773No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTCG7050.16931648974494454No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGCTGCGCCTGATCCTACTAG6840.1642730198376483No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGTGCCTGATCCTACTAG6240.14986310581680196No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATAATTGTTGCGCCTGATCCTACTAG4640.11143666842787839No Hit
AGGGCTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG4590.11023584225947451No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGATTGTTGCGCCTGATCCTACTAG4540.10903501609107065No Hit
ACAGGTCTGCCGCCTTGCAATGCGACGCCTGCCTGGGAGCGCAGGTGAGG4390.10543253758585906No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTTCGCCTGATCCTACTAG4320.10375138095009366No Hit
AGGGTTCCCCGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG4270.10255055478168981No Hit
AGGGTTCCCTGGCCCCAGTAGTCCAAAATTGTTGCGCCTGATCCTACTAG4210.10110956337960517No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCCGATCCTACTAG4170.10014890244488207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)