FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429989
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG34958481.30068443611349No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGCCCCTTGG39900.9279307145066501No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACATTGTTGCGCCTGATCCTACTAG33430.7774617490214866No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC28160.6549004741981772No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAACTTGTTGCGCCTGATCCTACTAG22110.5141992004446625No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGCTCCTACTAG10740.24977383142359455No Hit
ACAGACAGGCCACCCAGGGCTCAGGCTGGGTATGGTGGGCTCTGGTGGGA7740.18000460476895921No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTCCTAG7660.17814409205816892No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGCTGCGCCTGATCCTACTAG7100.16512050308263698No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGTGCCTGATCCTACTAG6350.14767819641897814No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTCG6340.1474456323301294No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTTCGCCTGATCCTACTAG5000.11628204442439224No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATAATTGTTGCGCCTGATCCTACTAG5000.11628204442439224No Hit
AGGGCTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG4860.11302614718050927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)