FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375359
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG7396019.703803558726445No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC37681.003838991472164No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA10830.28852378656166494No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC7470.19900948158962486No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACATTGTTGCGCCTGATCCTACTAG6630.17663090534661482No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG5750.15318668261584245No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCGTCACGTCGTTCCAGTTATC4810.12814399015342645No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAAAT4790.12761116690954527No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCTATTACTATCATAGTAAT4590.12228293447073335No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAACTTGTTGCGCCTGATCCTACTAG4370.11642187878804025No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATCAGACGAGCTGCT4230.11269211608087192No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTGTAGGG4220.11242570445893132No Hit
ACTGTCCCTTGGCCCCAGATATCAAAAGCATCATATCTTCCCAGCCACGT4210.11215929283699072No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCCCCAATTGTAGCCATCTCC4210.11215929283699072No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCACCGCCCCCAACTCTCGC4150.11056082310534714No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGCCGGCTCTCGCACAGTAATACAC4150.11056082310534714No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATTTCCCGTTCCCCAGTCACTGGC4140.11029441148340655No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGAAAACG4130.11002799986146596No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATAC4090.10896235337370358No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCGCCTTATAGCAGCTGGT4070.10842953012982239No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTGAGAA4010.1068310603981788No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCGACGTTACCACCGTAGTC4000.10656464877623821No Hit
ACAGACAGGCCACCCAGGGCTCAGGCTGGGTATGGTGGGCTCTGGTGGGA3950.10523259066653524No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCCGGGAGTTACCAC3950.10523259066653524No Hit
AGGGTTCCCTGGCCCCAGTCAGAACTGGGGAGCTCCCACCTTGCTCTCGC3940.10496617904459464No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTTCAGCTGCTGCTAAAGA3930.10469976742265405No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAACCCCG3920.10443335580071346No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATGCCATAG3900.10390053255683226No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATGCGTA3870.10310129769101047No Hit
ATTGTCCCTTGGCCCCAGATATCAAGAGCATCACACCTGCCACTACTGGG3860.10283488606906988No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCGAGCTCCCATCCTCTCT3860.10283488606906988No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGCGAGACC3830.10203565120324809No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGGTATTCCCCGAACCATA3830.10203565120324809No Hit
GAGGTCCCCTGGCCCCAGACGTCCATCCCATAATAATAGGCCGTAATCAT3810.10150282795936691No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGGACACCCTCCCGTACCAGCT3800.1012364163374263No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATTTGGCCTGACCCACGCTGCTATG3780.10070359309354512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)