FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432686
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC39230.9066621060075898No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA11230.25954156131698275No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG9390.2170164969516No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC8510.1966784226898952No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG7320.1691757995405444No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCACGCATCATAGTAAGATAA6490.14999329768007286No Hit
GTGGTCCCTTTGCCCCAGACCTCCATGTAGAAGTACTAGTCCTCATAAGT6440.14883772527883962No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATGAAGTGGCAGCTACCA6390.1476821528776064No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATCT6250.14444655015415336No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATTCGTAATTATA6210.14352209223316678No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAATA6140.14190429087144027No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCGTAACCATAGCTGTATCC6050.13982426054922045No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATGGTAGACTAATACAATATCTCT6030.13936203158872715No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCGGC6020.13913091710848052No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGAACGGGTAGT5980.13820645918749394No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATTGCTTTGG5870.13566419990478082No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTAAGTTC5820.1345086275035476No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGGGCATA5750.13289082614182107No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCCGAGATAATAACC5590.12919299445787477No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCATGATGCTATGGG5510.1273440786159016No Hit
GTGGTCCCCTTGCCCCAGACGTCCATGTAGTAGTATGAGCCTACTAATCC5500.12711296413565495No Hit
GTGGTCCCTTGGCCCCAGACGTCCTCTGGGTTAATAACCACTCCAAAAAT5470.12641962069491502No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATGGAGGGCCCTCGCACA5420.1252640482936818No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGTCTCGCAGGGCTCGCG5390.12457070485294185No Hit
ACGGTGCCCTGGCCCCAGTGCTGGAAGTATCTAGTGCAGCTGGTTGGACT5390.12457070485294185No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAGTAGTGAGAATCACTTCTATCATA5370.12410847589244857No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCATTTCG5360.12387736141220192No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCCGAAGGTTGTATAGCATA5360.12387736141220192No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTGCCACTCCAAAAATC5340.12341513245170864No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGGAGTTGTAAGAATACTC5320.12295290349121533No Hit
ATGCAGACACCACACGCTCTCCTGCAGTAACTCCTTTCATCTTCACGGTG5310.1227217890109687No Hit
AAGGTTCCCTGGCCCCAGAAGTCAATATGCGGGTTAGTAGCTCCCATTTC5300.12249067453072204No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACGGAAACCC5280.12202844557022877No Hit
AGGGTTCCCTGGCCCCAGTACCGGTAGTCACCGTAGTCTGGTGCAGCTCT5270.12179733108998211No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTGACTATTGGCGTCTCTCACACA5260.12156621660973546No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCCGT5250.12133510212948882No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAGGGCGTAGTCCGAATCTCTCGCACA5250.12133510212948882No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACTCGGATCTCACTCCAAA5240.12110398764924217No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCCCTCCGATGCCATGTCT5230.12087287316899552No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATTGCACCTTCGGACCAGTCAAAATA5190.11994841524800895No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTCGTCACCAAAATCGCACA5190.11994841524800895No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCTAAGGGATACCAGCCACT5190.11994841524800895No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGAGTGGGTAGCAGCTACCACC5180.1197173007677623No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATTCATAAGTAGT5180.1197173007677623No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCCGTCGA5150.11902395732702237No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAAGTCGAGCTGCT5140.11879284284677571No Hit
AGGGTTCCCTGGCCCCATCCGTAGTCACTGCTCGCACAGTAATACACGGC5140.11879284284677571No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCAAGAGCAGCTACCACCTC5130.11856172836652909No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCCGCGCTTGAGCTGTATCCTTCTCT5130.11856172836652909No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCAGACTCGCTATA5120.11833061388628244No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTGGACC5120.11833061388628244No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAAGAGGGGGAATA5110.11809949940603578No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGATAACC5110.11809949940603578No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGTCATC5080.11740615596529584No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGCGATCCCAAA5050.11671281252455591No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCTTTCCCCATACCGTATAA5050.11671281252455591No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATAATAGTAGGGGTAGCAGTC5030.11625058356406262No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGCATGAACCATAGCTGGATCCCGA5030.11625058356406262No Hit
AGGGTTCCCTGGCCCCAGTAGTCGGAGTGACCGCCGTAGATGCGCCTCGT5000.11555724012332269No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGTACC4990.11532612564307604No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCGCTTTTGGCAGCT4990.11532612564307604No Hit
AGGGTCCCCTGGCCCCAGTAGTTGATGTGGTACCAGTTGTTGCTGGTGTA4970.11486389668258275No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGATCGTTCCAGTTATAAT4960.1146327822023361No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGGGCGCTCCAAAAATCGTAATTAG4950.11440166772208947No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGAGTTCCCCACGAC4940.11417055324184282No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGACTATAACCAGTCAAAA4920.11370832428134953No Hit
GTGGTCCCTTGGCCCCAGACGTCCATCGATGTGGAATAACCACTCCAGGG4860.11232163739986967No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCAGAGCAGGT4860.11232163739986967No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCGTCCACCCAA4850.11209052291962301No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCACCGTTCCC4830.11162829395912972No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCAAGTGGTAGCAGCTA4810.11116606499863642No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGGACGTA4800.1109349505183898No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTACCAGGGGGTAAT4780.11047272155789649No Hit
AGGGTTCCCTGGCCCCAGCTCCCACTCCCCGAACCATAGTAGCTATGATC4760.1100104925974032No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCAACTCTCGCACAGTAATACAC4760.1100104925974032No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGAGCCACT4760.1100104925974032No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAATC4750.10977937811715655No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGATAATCATGATAACCAGTCCT4750.10977937811715655No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCACCTAT4730.10931714915666327No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGGACCCGCGTA4720.10908603467641662No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCGGCCTC4710.10885492019616996No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTCCTCCCCTCGTTCCAGT4700.10862380571592332No Hit
AGGGTTCCCTGGCCCCAGTAGACTAAAACCAAGGACGAGCTGCTATGGGT4700.10862380571592332No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTTCCGAGCTGCTGAGACTCT4700.10862380571592332No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGAGATG4680.10816157675543002No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAAGGCCACCCT4660.10769934779493674No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCACGAAAATGGCAGCAGC4640.10723711883444345No Hit
GTGGTCCCTGTGTCCCAGACGTCCATGTAGTAGTAGTAGTAGTAAGGGGG4640.10723711883444345No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCAGCCACGGTGAGTCAGCC4630.1070060043541968No Hit
AGGGTTCCCTGGCCCCACTCCACTGAACCCGGACAGAGCCGTACCAGCTG4620.10677488987395015No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCCCCATCATAGCAGCT4610.10654377539370352No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGGGTTCCCGGGGGGGA4600.10631266091345687No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCAGCTCCCACTATAGA4600.10631266091345687No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCTAGGGACGA4600.10631266091345687No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTTAGCGGGTGCCCCGCC4590.10608154643321022No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTACTGGAATCCCAGTGCCCG4580.10585043195296359No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATTGGTAATAACCACTACTATCATA4570.10561931747271694No Hit
ATGGTTCCCTGGCCCCAGTAGCCAACTGGCACTGCTAAACCGGGTCTTAA4560.10538820299247029No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACTTCTCACCACTACTATCAT4560.10538820299247029No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAGTAACCCGAAATTATCACTCCCTGA4550.10515708851222365No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTATAACCATC4550.10515708851222365No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCACCCTGGTTTTCTCTCGC4540.104925974031977No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCTGGACCA4540.104925974031977No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTTTTCAGAATCGGA4530.10469485955173034No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTATATCACCCGGCCAGTGTAGCT4510.10423263059123707No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGGGTGAGTAGTCACCGTATAGGTC4510.10423263059123707No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAAGA4510.10423263059123707No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCAAGAACCGTAGTCCGGTGGGCCGCT4500.10400151611099041No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTAGTCCCCCAGCCACTGCT4490.10377040163074377No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTACCCTCCCCC4490.10377040163074377No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATTCCGAACC4490.10377040163074377No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGACGATCACCGTAGCAGCTACCACCA4490.10377040163074377No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAACCTTCGTAATAACCAGT4470.10330817267025047No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCGGAACGATAACTCCCCCA4460.10307705819000385No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCGGAGGGGTAG4460.10307705819000385No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGGTGCTACCA4450.10284594370975719No Hit
AGGGTTCCCTGGCCCCAGCAGTCAAGGTACGATAGTCCCAGCTGCCGGTC4450.10284594370975719No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATTGGGT4440.10261482922951054No Hit
AGGGTTCCCTGGCCCCAGTGTTCAAAGTTAATACCAGCCACTGCCACCCA4440.10261482922951054No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAACATTGACCAGCTGCTGCTATACC4430.1023837147492639No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTATGAGGAGCAGCTGGT4420.10215260026901725No Hit
TCTGTCCCTGGGCCCCAGAAATCAAAAGAGTCAGGGGAGGATCCACTACT4420.10215260026901725No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGTCATGGTTTCGGGCAACTGG4420.10215260026901725No Hit
AGGGTTCCCTGGCCCCAGAAGTCAAAATCGTATGCCAGCCAGCGACAGAA4420.10215260026901725No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTTGGAATC4420.10215260026901725No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAACGGGA4420.10215260026901725No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCCGACGGATCTAA4420.10215260026901725No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTGATACCTGGACACTACTAT4410.1019214857887706No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCTCCCACT4410.1019214857887706No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACCCCCCCCGCGTAGCGATCACATCT4400.10169037130852397No Hit
AGGGTTCCCTGGCCCCAGTAGACCAGGGCGGGATAATAACCAGTCAAAAT4380.10122814234803067No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCCCGTGT4370.10099702786778404No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCGAGGTAGTAATAACCACT4360.10076591338753739No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCGAGACTTCCCGAACCCCC4340.1003036844270441No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCCGGGG4340.1003036844270441No Hit
GTGGTCCCTTGGCCCCAGACGTCAGTTCCACCCGGAACTCTCGCACAGTA4330.10007256994679745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)