FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394812
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC45431.1506742449570935No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA12460.31559324437960345No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATCG9550.24188727799560297No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGTCACC7460.18895069045520402No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAACC7060.17881928614125203No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCAGCTACC6830.17299372866072968No Hit
CCATCATAGCCCTGCTGACCCTGTGACCTTGTGGCAGCCCCTCTGGCCCC6750.1709674477979393No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG5760.14589222212090816No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATTGCACGATCGAATAATCACTACC5720.14487908168951297No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGATACGTAGCCACC5330.13500096248340981No Hit
ATGTTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACGCCGAGCTGCTATA5240.13272139651277062No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCCATAGGTA5160.13069511564998024No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGTGGTAG5140.13018854543428265No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCGTAGTCACCGTAGTCCCC5110.12942869011073624No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCTAAGCAGCTGGTACTACT5010.12689583903224827No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAACCCCCCGAACC4980.12613598370870188No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGACACTTTCAACCC4970.12588269860085308No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAGCGAAAATTCGCACAGTAATATAC4960.12562941349300427No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAACTGTA4880.12360313263021389No Hit
AGGGCTCCCTGGCCCCAGTAGTCGTAGCTCCCACTCGCCGCACAGTAATA4860.12309656241451628No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCCGGACGTCCCCCAGGGTCT4850.12284327730666747No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCAAGCCCCGTACCAGCTGCTGCTATC4850.12284327730666747No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGTCCCTTTGGTAACCACTACT4840.12258999219881869No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAACTATAGCA4830.12233670709096989No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTAGGTTTC4820.12208342198312108No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATGGGTA4820.12208342198312108No Hit
GTGGTCTCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCCCCATCTCT4810.12183013687527228No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCTCCTTAATAA4740.1200571411203307No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTTGGTTGGTGCCAGCCACTGCTAC4730.11980385601248189No Hit
GTGGTCCCTTGGCCCCAGACGTCCCCAAGACCCTCGTAGTAACGCTCTTT4720.11955057090463309No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTACCAGCCACTGCTATA4690.1187907155810867No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTAAG4680.11853743047323789No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCCTCAAAATACCCTGAGGGGGC4660.1180308602575403No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCTTCTGAATCTCTCGCACA4650.1177775751496915No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAAGGAGTCACCGTAACT4650.1177775751496915No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAATTTTTCCCAGTCGACAAATCTCT4630.11727100493399391No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGGGTCAGCTGCT4600.1165111496104475No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATTTTCCGAACCATAGTAA4600.1165111496104475No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCTTCCGCCGGGTAGGTCTCTCGC4580.11600457939474991No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACTCATAGAAGCTTTGGTCCGTGC4580.11600457939474991No Hit
AGGGTTCCCTGGCCCCAGTATGCCCACTCCAAAACGGACCCCGAGGGGTT4570.1157512942869011No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATTCCGTAATCTCTCGCACAGAAATA4560.1154980091790523No Hit
AGGGTTCCCTGGCCCCAGTATCTCAGCCCCCGTCGATCTCTCGCACAGTA4550.11524472407120352No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTTTTCCCC4540.11499143896335472No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACGGGACCCCCGCGGACTAACTCCCC4530.11473815385550591No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCCAAGTA4520.1144848687476571No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAAATAATA4520.1144848687476571No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATCCGCCGGGGGGCCACTC4510.1142315836398083No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTCGACGG4480.11347172831626191No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAACCAAAACT4480.11347172831626191No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGCGGAGGCA4480.11347172831626191No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGACCCCCTG4450.11271187299271553No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAGTAGTAGTATCCCCCACG4440.11245858788486672No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTGGAGACCAGCCACCT4440.11245858788486672No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGATC4410.11169873256132033No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACTGCAGGCCCGTGTA4410.11169873256132033No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTTCCAGCTGCTGCTGCC4400.11144544745347153No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTGCCGATA4400.11144544745347153No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACCCCAC4400.11144544745347153No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGATCAGAGT4400.11144544745347153No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCCGAATA4380.11093887723777393No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTACACGTAGTA4380.11093887723777393No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCTTGGGTG4370.11068559212992513No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCTCGGTCACCGTCCGGATCTCT4370.11068559212992513No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCCTAGGGAGGGGACCACTCC4350.11017902191422753No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCAAGGGTAGTA4350.11017902191422753No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGAGGGTA4340.10992573680637874No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCGTTAAC4340.10992573680637874No Hit
GTGGTCCCTCGGCCCCAGACGTCCATACCGAAGTGGGAGGCCCAGACCCC4330.10967245169852993No Hit
AGGGTTCCCTGGCCCCAGGGGCCATACATATAGCAGCTGGTACTACTACA4330.10967245169852993No Hit
AGGGTTCCCTGGCCCCAGTGGTCAAAGTAGTGTTGGCCATAATCCTCTCT4320.10941916659068113No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGAGTACCGTGAGGAATA4300.10891259637498354No Hit
ATTGTCCCTTGGCCCCAGATAGCGCAATCACCACCACAATCAGGACTCGC4290.10865931126713474No Hit
AGGGTGCCCTGGCCCCAGTGTTGGAAGTACTCCACATAATAACCATTCCA4290.10865931126713474No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACGCGTAGCTCCCACTATA4290.10865931126713474No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAGTCCAGAGAAGTATAT4290.10865931126713474No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTCCGTAGTAGCCCATTACCATAGG4290.10865931126713474No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTATTCCGT4260.10789945594358835No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACCTTCC4250.10764617083573955No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGACAAATATAATAACCAGTCAA4250.10764617083573955No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGGTCCAGTG4240.10739288572789074No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGGGTTGTAAC4240.10739288572789074No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCATCCCCGAATAGCTCCCAC4230.10713960062004194No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACCCTTTGGTCCGACTATA4230.10713960062004194No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCACCACTCCCGACTCCGGAGGT4220.10688631551219316No Hit
AGGGTTCCCTGGCCCCAGTAGTCCAAGAAGACACCGTACGGGCGCCTCGC4210.10663303040434435No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGCCGTAGTAGTAATACTTACTGTA4210.10663303040434435No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACCAAATAGATTTCATACA4190.10612646018864674No Hit
GGGGTCCCTTTGCCCCAGACGTCCATGTAGGGGTAGAACCAACCAAAACT4180.10587317508079794No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGTCACCGTTAAATCGTTCTCTCGC4170.10561988997294916No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTTTGGA4170.10561988997294916No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTCCGTCCC4170.10561988997294916No Hit
GTGGTCCCTAGGCCCCAGACGTCCAGACCATAGTAATAGAAATATCCATC4160.10536660486510036No Hit
GTGGTCCCTTTGCCCCAGACGTCCATTAAAGCGGACCTGGGAACCACCAC4160.10536660486510036No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAAAAATTATCGGGTAGTCGGTC4160.10536660486510036No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTATTCGGTAGC4160.10536660486510036No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCTGGCGCATAATAACCAC4140.10486003464940274No Hit
AGGGATCCCTGGCCCCAGGGGTCGGACCTCTTAGGCTAATAATCACTACT4140.10486003464940274No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCTAGACTACTATCATAGTAATA4140.10486003464940274No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTGTG4130.10460674954155394No Hit
GTTTTCCCCTGGCCCCAGACGTCCATACCATAGTAATACAAGTGAGACGT4130.10460674954155394No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGGTCAACATCGTAATAACCACT4120.10435346443370516No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTTATAACC4110.10410017932585636No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCTATAACCAT4110.10410017932585636No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGACCGGT4100.10384689421800755No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGCCTTTTGGTC4100.10384689421800755No Hit
AGGGTTCCCTGGCCCCAACAAAATAATAACCAGTCAAAATATCGTAATAC4100.10384689421800755No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACCAGTCGTTCCAGTTGTACCCCCTT4100.10384689421800755No Hit
AGGGTTCCCCCGCCAGCGTCGAAGAACCAGTCAAAATGGGACCGTGTCGA4090.10359360911015875No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAGTAACCAGCGTATATAGTGGTACA4090.10359360911015875No Hit
TGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTCCCCTGTCGAAACTAC4080.10334032400230996No Hit
CAGGAAGGGGCTCTGGTGCCGGGCTGAGCGGGGGAAGCAGGGGTAGCGGA4080.10334032400230996No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAGTGTCCATTACGGGGGCC4080.10334032400230996No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCTCCCATAAG4080.10334032400230996No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCTCTTCCCCG4060.10283375378661236No Hit
AGGGTTCCCTGGCCCCAGTAATCACGGGAGTCGTTGTCTTCTCTCACACA4050.10258046867876357No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACCCCGT4050.10258046867876357No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAAAGAGTAGCTCC4050.10258046867876357No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGAGGAATACACGACCATGTCTTCA4040.10232718357091476No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGCTCCGCTATAGCTCTTGCACAGTA4030.10207389846306597No Hit
AGGGTTCCCTGGCCCCAGTAGTCCTCCAAACTCATCCTTGTAGCCATCTT4030.10207389846306597No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGTAGAAGTCCGAATTCACGTA4030.10207389846306597No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGACGAAGCTCCCACA4030.10207389846306597No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCTGAAAACCAT4020.10182061335521717No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCCGAGAG4020.10182061335521717No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCCCGTGGTAGCTCCCACTAT4010.10156732824736837No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCACTGTAACCACTACTATC4000.10131404313951957No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCGCAGTAGTAGTAGGTGGCAGT3980.10080747292382199No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGGACCCCCGAAAATTACG3970.10055418781597318No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTCTCTCCTTGTGGGTAGC3970.10055418781597318No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTATCTCGACACCGTCAGCCCCTC3960.10030090270812438No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGGGAGTAGCA3960.10030090270812438No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGGTAGTAGCTCCCACTC3960.10030090270812438No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATGGACCCTTGTCTTTCTCGC3960.10030090270812438No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACAAGGGGTGGGATTGGTG3960.10030090270812438No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGAGACTGATCCCCATGCGGCGCC3950.10004761760027557No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTAATAAC3950.10004761760027557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)