FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410942
Sequences flagged as poor quality0
Sequence length300
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC7215117.557465530415485No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATTTTTCGGCGGGACCAG259766.321086674031859No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGAGGGAGTAGTCAAGGTCGCACAGTA6270.15257627597081827No Hit
AGGGTTCCCGGGCCCCAGGGGTCGAAAATCCCAGGCGAACCAACTGTTGC5100.12410510485664644No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATTTTTCGGCGGGACCCG4960.12069829805666007No Hit
TTGGTCCCATTGCCCCAGACGTCCATGTAGTAGTGGGCTTGGTAGTATAA4730.11510140117096818No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCAGAGCCACTGCTCGCGAAAGA4720.114858057828112No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATACCAGCTGCTGCTATACCG4650.1131546544281188No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCGCGCTGCCCGAGGCTCT4630.11266796774240648No Hit
AGGGTTCCCTGGCCCCAGTAGTCCACCAACCCACTACTATCATAGTAACT4560.11096456434241328No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGCCCCAATCGTAGCCACCCTGCC4530.11023453431384478No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCGTAGCTTCGCAGCTACC4480.10901781759956393No Hit
GCGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTACTTGTACCCGGTCGC4460.10853113091385158No Hit
GAGGTCCCTTGGCCCCAGACGTCCAGACCGTAGAAGTAGTTGTAGCCACC4400.10707107085671458No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCTCTCCCCGAGAATATCTCGC4380.10658438417100223No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCCAAGGGGTGGGGATGTAAT4380.10658438417100223No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTGGCCACTGAC4380.10658438417100223No Hit
AGGGTTCCCCGGCCCCAGACGTCGTAATAACCACTATCTTCATACCAACC4320.10512432411386521No Hit
ATTGTCCCTTGGCCCCAGATATCAAAACCATAGTAGAGACTTCTCGCACA4300.10463763742815288No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAATAGTGGAGGTAACCATA4240.10317757737101586No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGGTCGGACGAG4220.10269089068530352No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGACCAAGTATCATA4170.10147417397102267No Hit
GTGGTCCCTTGGCCCCAGACGTCCACGAACACTACTATCATTAAATGTCT4150.10098748728531035No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTTAAAATCGTA4140.10074414394245416No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCGAGGGCACCGTAGTCATA4120.10025745725674182No Hit
ATTGTCCCTTCGCCCCAGAGATCAAAAGCATCACCACCAAAGCTGTTCCC4120.10025745725674182No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTACGGATAGCA4120.10025745725674182No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTTCCCCGTAGAACCCCA4110.10001411391388566No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCTTAGCAGCTGGTACTAC4110.10001411391388566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)