FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434740
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGAGATAGCAGCTGGTACTAC19880.45728481391176334No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAACC12090.278097253530846No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCTTT7210.1658462529327874No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCTCGACCCTCCCTGAACCATAGT7110.16354602751069605No Hit
AGTATTCCCTGGCCCCAGGGGTCGAACCAGCGAGTAGCAGCAACCGCCAC7000.16101577954639554No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCAGGGACCACTCCAAAAA6890.15848553158209502No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATAAATGGTAATAACCACTACTATC6660.15319501311128492No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGGGCCACCGTAGTCTCTCTCGC6650.15296499056907578No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCATGGCCTCCCTTTAT6560.15089478768919354No Hit
AGAATTCCCTGGCCCCAGTAGTCATCGCTTTTGGCGCGAGAACAGGTCGC6480.14905460735152046No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGGAGTAGGAGTAGTACCAGCCACT6420.14767447209826562No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTACTTAGCAGCTGG6410.14744444955605648No Hit
AGCTCTTGGCGTTGTCACTGGAGATGGTGAATCGGCCCTTCACAGAGTCC6300.144914201591756No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGGTTGAC6260.14399411142291943No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATGT6250.1437640888807103No Hit
AGGGTTCCCTGGCCCCATTCTTCTGAGTAATAACCACTACTATCATAGTA6110.1405437732897824No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATCCAGCTGCTGCTGCTGC6110.1405437732897824No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGCGGTA6010.13824354786769105No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACGAGCTGCTATG5990.13778350278327275No Hit
AGGGTTCCCTGGCCCCAGAAGTCAAATCCCCTGGAGCAGCCAACACCACA5990.13778350278327275No Hit
AGGGTTCCCTGGCCCCAGCTATCGTAGTTCCCCACGCCTAGGGCGAGCGT5980.13755348024106362No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGAGTAGTT5960.13709343515664535No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATAATAGAAGTAGTCCGTAGT5910.13594332244559967No Hit
GTGGTCCCTTTGCCCCAGACGTCCAAGTGGTAGTAGTAGTAGGACACACA5910.13594332244559967No Hit
AGGGTTCCCTGGCCCCAGTAGGCAGAGCAGCCGTTACTAGTACAATAACG5840.1343331646501357No Hit
AGGGTTCCCTGGCCCCAGTAATCAAGCTTAGTATTTCCCGCAAGATCTCT5820.13387311956571743No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGTCCCGGTCCCACTTGCACAGTA5810.1336430970235083No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCATAATAATAATAAGGCCAAAA5800.13341307448129916No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACCCCCC5770.13272300685467175No Hit
ATTGTCCCTTGGCCCCAACTATCAAAAGCATCCCCCCGGTTCCAGCCAAC5760.13249298431246262No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTACCACCGAGCTGCCCTATC5680.1306528039747895No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGTCC5680.1306528039747895No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCCCCAAC5670.13042278143258038No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTACC5630.12950269126374384No Hit
GTGGTCCCTAGGCCCCAGACGTCCATACCGTAGGAGTGACCGTAATAAGT5610.12904264617932557No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGGGTACTCCATA5610.12904264617932557No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACGATTCTATTCCTCTTGCACAGTA5580.12835257855269816No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATGTGATGAGAATACTCCCTG5570.12812255601048902No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGGGCGGGGAAGGTGATAACTCCC5570.12812255601048902No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTAGATAACCACTCCTT5550.12766251092607075No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCCCCCGCCTGATCACCGTATTCGGT5490.12628237567281594No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTAGGTCG5420.12467221787735198No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCGCCTCCTTGGTACGT5410.12444219533514285No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATAGAGGTTC5400.12421217279293371No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAAGG5390.12398215025072458No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTTTGGTT5390.12398215025072458No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGACTGACGGTAA5380.12375212770851544No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGGCAGCTACCACCACTATTTGACT5350.12306206008188803No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCCCTCGATCGGTCGCTCTCGC5340.12283203753967889No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTCCACCCGGCGTAGTCACCGTA5320.12237199245526062No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACTTCGTGGGATCTTT5320.12237199245526062No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGGAACAACGTAGTTACTGTA5290.12168192482863321No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAAAAAAGATAACTCCCTCTTCT5280.12145190228642408No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTGATTTT5260.1209918572020058No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCTAAGTAGTAATAACCACTACT5250.12076183465979666No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAGCTGCT5230.12030178957537839No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGACTCCCGTAGTCAT5230.12030178957537839No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGCAGTACCTGTTGAATCTTCTCTCGC5220.12007176703316925No Hit
ATTGTCCCTTGGCCCCAGGACGAGTAATAACCACTACTATCATAGCCTCT5220.12007176703316925No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGCCACATATGCCCCTCCAAA5180.1191516768643327No Hit
AGGGTTCCCCGGCCCCAGGGGTCGAACCACCCAAAGCAGCTGGTACGACT5170.11892165432212357No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTATAGTATTGGGCCCAGCC5170.11892165432212357No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCATCCCTTACCAGCTGCTGCTAC5170.11892165432212357No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGCTCC5160.11869163177991443No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGTGTTGA5140.11823158669549616No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACGCTAACTG5120.11777154161107789No Hit
AGGGTTCCCTGGCCGCAGTAGTCAAAAGGGGCGTAGAAGCTCCCACTCAC5120.11777154161107789No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAATGAGTGGAAGG5120.11777154161107789No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTCCAGCT5110.11754151906886874No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCGACCGACCCTCTCGCACA5090.11708147398445047No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTGGGTTTGCTCTTC5090.11708147398445047No Hit
AGGGTTCCCTGGCCCCAGTGAAGAAAGGAGTAGCAGCTACCACTCCATCT5090.11708147398445047No Hit
ATTGTCCCTTGGCCCCAGATATCAAAATCATAATTTTTGTAGCCAGGGAG5050.11616138381561393No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCGAGAAAGTGACACAATAATA5040.11593136127340478No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAATACGT5020.11547131618898651No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGTCCGCGTAGT5020.11547131618898651No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGTTCCCG5000.11501127110456824No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTGTCCTCCGTCTCCCACTGC4990.1147812485623591No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCAAATAACCAGTCAAAATATCG4970.11432120347794084No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGCTCCCAAACCCATC4970.11432120347794084No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACCCAGAGGTGGGGCAGCT4960.1140911809357317No Hit
AGGGTTCCCTGACTCCAAACGTAATCATTGGCACCATCTTTCGCACAGTA4960.1140911809357317No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGACGTTATAATAACT4950.11386115839352255No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGGAGTAACCTGGGTCTGCCCT4950.11386115839352255No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACGGAAACCC4940.11363113585131342No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATCGAAATGACGTAGGGGG4930.11340111330910428No Hit
AGGGTTCCCTGGCCCCAATCGTCGTGGACCGCCACGTAATCACCCTACCA4930.11340111330910428No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCCCGTAGTT4930.11340111330910428No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAAATAAACCATAGCCGC4910.11294106822468601No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGATAGCA4910.11294106822468601No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATTTTTCGGCGGGACCAG4900.11271104568247688No Hit
AGGGTTCCCTGGCCCCAGTAGTCTATTCAGCGACGAGTAGTAATAACCAC4900.11271104568247688No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACGCAGAATAATAACCACTCCAAAA4900.11271104568247688No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTGCGCCATTTTTAACCCGGT4900.11271104568247688No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAACCGTAGTAGTGGTCGTCGGGGTA4880.11225100059805859No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCGGGGTAGTA4870.11202097805584946No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTCGATAGCAGCTGGTACT4870.11202097805584946No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGGTGTTTAGTCACCGTAGTGAG4860.11179095551364035No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGCGAGCTGAAAAATATC4860.11179095551364035No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAAGAAGAATA4850.11156093297143121No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTGGGTCATAAC4830.11110088788701293No Hit
AGGGTTCCCGGGCCCCAGTAGTCATAAAAGGCGGTCGCACTCAGTTTTTC4820.1108708653448038No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCCAAAAG4800.11041082026038553No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTCTATCCA4790.11018079771817639No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGGCCAGC4770.10972075263375812No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAAACCATAGTAATCCT4770.10972075263375812No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAAGGGCCTAACCAGTCAAA4760.10949073009154899No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTCTTCACTTACCAGCCA4750.10926070754933984No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCTAAGCCACTGCTATACCCGGATTTT4740.1090306850071307No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCTTCGGGCGGGTAACCACTAGA4740.1090306850071307No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACGGGACTTTCCCCGA4730.10880066246492157No Hit
AGGGTTCCCTGGCCCCACCACTCGAACGTACCAGCCACTGCTTTACTCTC4730.10880066246492157No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGAAGAAAGTAATAACT4730.10880066246492157No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAAAGAG4720.10857063992271243No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTAACTAG4710.1083406173805033No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACAATCGCGGTTGCGAAAGTA4710.1083406173805033No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAATAGAAAC4700.10811059483829416No Hit
GTTGTCCCTTGGCCCCAGACGTCCATACCGTGAATTCGCGATATAGCAGT4700.10811059483829416No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCGTCCCCTGTAGCAGCT4700.10811059483829416No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCTTATTCT4690.10788057229608503No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGAGA4680.10765054975387589No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCTATCAGCAGCTGGTACTCCCTG4670.10742052721166674No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTTGAAGTACCAGCT4670.10742052721166674No Hit
TCACGGGGAACCCTGGATGAGAAGCTACGGTGTAAGTCCTGCCCCCTTGG4660.1071905046694576No Hit
AGGGTTCCCTGGCCGCAGTATTTCGGTATTATAATAACTACTATCATAGA4650.10696048212724847No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATTTCCT4650.10696048212724847No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCTTTAATAACCAGTCAAA4650.10696048212724847No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATTTTCCGGGTTC4640.10673045958503934No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAGTAGCTATAAATACTGGG4610.10604039195841193No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCACGACCCCC4610.10604039195841193No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGATAATAACTCC4610.10604039195841193No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAATAGGGTCGGGGAAG4570.10512030178957538No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACGGGTAGCTCCCACCTCCCGACCG4570.10512030178957538No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGGAACC4550.1046602567051571No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGACTGTAGCCATCTCGAACTCT4540.10443023416294797No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACTCCAG4540.10443023416294797No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTATAGCGT4520.1039701890785297No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTACTCCGT4490.10328012145190228No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAGTACGAGTATAATAACCA4490.10328012145190228No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTTGGACGGAGCTTT4480.10305009890969315No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAATTGGGAGGTCTCTCCTCCCCCACT4470.10282007636748401No Hit
GTGGTCCCTGGGCCCCAGACTTCCATACCGCACGCGTTGCAATTGACATG4470.10282007636748401No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATCCTCCGGGGGGGTACCAGCTGCT4460.10259005382527488No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTATAACCACTCCAAAAATC4450.10236003128306574No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTATCACCAACCACTACTATCATA4450.10236003128306574No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGCCCCATGGTCG4430.10189998619864746No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGCTTTTACTACCTCC4420.10166996365643832No Hit
AGGGTTCCCTGGCCCCAGTAGTTGACTAACCACTACTATCATAGTAATAC4410.10143994111422919No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCAACCCACTAACGATAACTCCC4410.10143994111422919No Hit
AGGGTTCCCTGGCCCCAGTGGCTAAGTAGTCACCGTAAACCTCCTCTCTC4400.10120991857202005No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTACTTCCT4400.10120991857202005No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCAGACATGACTCGGTCTTCTTC4390.10097989602981092No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATGGGTGTTTCGGGTAGCA4390.10097989602981092No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCGCCGGAGTTACCACCGTAGT4380.10074987348760178No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGAGGGGTAATAACTCCCC4370.10051985094539265No Hit
AGGGTTCCCTGGCCCCAGTAGTCTACGGCATTCCCTCCTAAGACTCTCGC4370.10051985094539265No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATAACGATAGCTGTATCTA4370.10051985094539265No Hit
GTGGTCCCTTGGCCCCAGACGTCCGGCCCATCCTGGAAACAGGAATACAG4370.10051985094539265No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCGAAACCCCTTCT4350.10005980586097436No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGGACCCTTGGGGTTACC4350.10005980586097436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)