FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443831
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGATGCT7620.1716869709416422No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAATAACCAGTCAAAATATC7230.16289984250762113No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTTCCCTCCTCCCGAAC6840.1541127140736001No Hit
ATTGTCCCTTGGCCCCAGACATCAAAAGCATCAGGTAGTAGCTCCCACTC6560.14780400647994393No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAACT6430.14487496366860358No Hit
GTGGTCCCTTTGCCCCAGACGTGCAGAATCCCCGAACCAAAGCCTCTCGC6410.14442434169762813No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTTTAAGAAGTGTGCGCC6240.1405940549443369No Hit
AGAATTCCCTGGCCCCAGTAGTCAAAGTGGAGGCCCGGTGGTAATATATC5830.13135630453934044No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATGGCCATCGGTAGCAGC5770.13000443862641411No Hit
AGGGTTCCCTGGCCCCAGTACCTCAACCACTCCAAAAATCGTAACCTGAT5740.12932850566995097No Hit
AGGTTTCCCTGGCCCCAGTGGTCGAACCAGCCTGTCGTTCCACTTATAGA5610.1263994628586106No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATTGGCATA5540.12482228596019655No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAAATAACCACTACTATC5500.12392104201824569No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCGGG5490.12369573103275797No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTGTAGTAGCTAAAATCGTG5480.12347042004727025No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGAGGCAGCTACCACCAC5470.12324510906178252No Hit
AGGGTTCCCTGGCGGGAATAATAACCAGTCAAAAGGGCTTTCGCACAGTA5460.1230197980762948No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACGCCCCTATAGCAGC5430.12234386511983165No Hit
GTGGTCCCTAGGCCCCAGACTTCCATGACATAATGGTATATTCCAGTCCC5430.12234386511983165No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATAATTTTGGGGACAGTGGTGAGCAG5390.12144262117788077No Hit
ATGATTCCCTGGCCCCAGTAGTCAAAGAAGTAAGAGTAACCGATCAAAAC5370.12099199920690533No Hit
AGGGTTCCCTGGCCCCAGGGGCCAAACTTCCCGAATGAGAGTAATACGTG5360.1207666882214176No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGCATCGTCGGGTACGGT5290.11918951132300357No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTACGTAGC5290.11918951132300357No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGACTTCACAGGTACC5230.11783764541007727No Hit
GTGGTCCCTTGGCCCCAGACGTCCACAGCGCCCACCTCCCCGAACCATAC5230.11783764541007727No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGTAGCAG5200.11716171245361409No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGCCACTGCTCCCCCCTTTTCTCTCGC5190.11693640146812638No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGAAGGACGAGCTGCTATCCCCTCT5190.11693640146812638No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCACCCCGGTCATAACCACT5190.11693640146812638No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGACCCCTATAATAACTCCCCGAAC5160.11626046851166323No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGGTAGCTTACCACCGTA5140.1158098465406878No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGATACTATCATACCTCC5140.1158098465406878No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCGTGAATCCCCAATTATAGCA5110.11513391358422462No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCCAGCTG5100.1149086025987369No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATCCCGGGGT5100.1149086025987369No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTGCCTCCCAC5090.1146832916132492No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTGAACTAC5080.11445798062776147No Hit
AGGGTTCCCTGGCCCCAGTTGTTTAGTAGTTCCAGTCCCCTCGCACAGTA5080.11445798062776147No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCGAAGTA5080.11445798062776147No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAACCGTAACC5070.11423266964227376No Hit
GTGGTCCCTTGGCCCCAGACGTCCTTTTCTATTAATCGAGTCGCACAATA5060.11400735865678605No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGGTAGTAGTCGTATGT5050.1137820476712983No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACGTAGTAGCTCCCACTGTGT5030.11333142570032287No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCAGGGAATTT4990.112430181758372No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATGGAGTACCATCTGCTGCTCATTCT4960.11175424880190883No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTTGTTCCCAGTCTCTCTGGC4950.1115289378164211No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGACGGGGTG4930.11107831584544567No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTTCGAGGGTTACCACCGAA4910.11062769387447025No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGTAGTAGTAGTCCGTAGTAGG4900.11040238288898252No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTAGCGTACGTAATCACCGTAGT4900.11040238288898252No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACCACTACTATCATAGTACCCGCTCT4890.11017707190349481No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAATCGTTCAATATCTCT4880.10995176091800707No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGAGCCATAGC4880.10995176091800707No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCTTCCCCCAAAAAGCCAG4880.10995176091800707No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGCCGCTCCCCTCCAATCCGCTCGC4870.10972644993251936No Hit
GTGGTCGTGGGACCTGAGTCCCACCTAGTCCTATTTTTTAGGGCATCCTG4860.10950113894703163No Hit
AGGGTTCCCTGGCCCCAGTGGTCAAACGTCGTAGTTCCACTCCAATTCTC4850.10927582796154392No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCGTGGTCAATGTAATCAT4840.10905051697605621No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGTGGTGACGATAATTCCCCCAATTG4840.10905051697605621No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGCAGGATATTGGGTTAAATAA4840.10905051697605621No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCTGTGGG4830.10882520599056848No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGGCCCACCGTAGTAAAGTCGGGG4800.10814927303410533No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCTAACGATAACTCCCCCAA4800.10814927303410533No Hit
GTGGTCCCTTGGCCCCAGACGTCCAGACCGAAGCCCTCGTAGTCACCGTA4800.10814927303410533No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATATGTTTTTCGGGTATCTCT4790.1079239620486176No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCAGTCAGGTGCTGCTAT4770.10747334007764216No Hit
AGGGTTCCCTGGCCCCAATGGTCAAAGCCCGTTACTCCCACTCTCGATTC4760.10724802909215445No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGGACCATAGCTGTATAC4760.10724802909215445No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAATCCCACC4750.10702271810666673No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACGAATCGAGGGGGTCGCCCCTCGCT4750.10702271810666673No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGTCCAGTCAAAAGATCGTAATAACC4750.10702271810666673No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCCAAAGGGGTTTGAATAACC4750.10702271810666673No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCGTTATCGGGAGAGTAGCAG4750.10702271810666673No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAACTGAG4740.10679740712117901No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCGCCGGTAGC4730.1065720961356913No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCCCAACCCG4700.10589616317922812No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCGGTAACCACT4680.10544554120825268No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCCCCCCGACGAGCTGCCG4680.10544554120825268No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTGACTCCCCGAACCAT4670.10522023022276497No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTGGTAGCCTCTCGCACAGTA4670.10522023022276497No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGTAGATT4670.10522023022276497No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGGTATAACCACTCCAAAAACCG4660.10499491923727726No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGGCCCC4660.10499491923727726No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACTGAGTTTGGGGGCCAGCTGC4630.10431898628081411No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCCCAAACGTAATCATATGGGG4620.10409367529532637No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTGCTACTATCATAGTAA4620.10409367529532637No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACAAATCATA4610.10386836430983865No Hit
AGGGTTCCCTGGCCCCAGTAGTCATCCCGGTTGTGACCCGTAGTCACCGA4610.10386836430983865No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCAGTACACCAAATAACAGCT4600.10364305332435093No Hit
AGGATTCCCCGGCCCCAGTAGTCATACCAGCAAATTGTTCCAGCACAATA4600.10364305332435093No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCGTAACCACTACTATCCC4590.10341774233886322No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTTAGTCCCCGAACCATACT4590.10341774233886322No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCCCGTGCTCCCAGCTGC4580.1031924313533755No Hit
GTGGTCCCTTGGCCCCAGACGGCCAAACCGTGAGGGTAATTTTCATCGAA4580.1031924313533755No Hit
GTGGTCCCTTGGCCCCAGACGTCCACGGAACCAGCCACTGCTGTCGGTCA4570.10296712036788778No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACTGAAACACGAAGTTACT4570.10296712036788778No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCCGTAGTAGATTCCAGC4560.10274180938240006No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGCCAT4560.10274180938240006No Hit
AGAATTCCCTGGCCCCAGTAGTCAAAGCCCCTCCTGGTACATACACCACC4560.10274180938240006No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACAAATTTTCCCCCAA4540.10229118741142461No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGATGGTGCTGGAGATCAT4530.1020658764259369No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTTCCACTCCC4530.1020658764259369No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCAGTTGT4520.10184056544044917No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTGTCGAGCTGCCGAACC4510.10161525445496146No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCTCCAG4510.10161525445496146No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCACGATAGTAGCTCCCGCC4500.10138994346947375No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTCCGTAGAAGAAGCAGCTGGAACT4490.10116463248398602No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTGCGAAATTGAAGTTAGAAGCCCA4480.10093932149849831No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCGAATA4460.10048869952752287No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATTCAGGTAATACACGAGGTG4450.10026338854203513No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGGGAGTATAATAACCACTCCA4450.10026338854203513No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGATATAGCTCCCACCACTAAACCC4450.10026338854203513No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGATAATCGTTGTCACTACCCC4440.10003807755654742No Hit
GGGGTTCCCTGGCCCCAGTAGTCAAAGTAAATAGTAGCTGGTACTACTAC4440.10003807755654742No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGAACCAT4440.10003807755654742No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGAAAAATAACCATAGCTC4440.10003807755654742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)