FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001132185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602956
Sequences flagged as poor quality0
Sequence length300
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGTAAAGCA601829.981159487591135No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGGACGAGCTGCTATT565979.386588739476844No Hit
GTGGTCCCTTGGCCCCAGACGTCTATACCGTAGTAGTAGTAGGTAAAGCA21490.35641074970644626No Hit
GTGGTCCCTTTGCCCCAGACGTCTATGTAGTAGTAGGACGAGCTGCTATT19940.33070406464153274No Hit
ACAGGTCTGCCGCCTTGCAATGCGACGCCTGCCTGGGAGCGCAGGTGAGG14350.23799414882678008No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGCCCAACCACTCCAAAA10510.1743079096982201No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCTCCGGGCTGGTACTACTAC7860.13035777071627117No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAGTAGTACCCGCCTATGACACTCGC7090.1175873529743464No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCGGCAGCTGGTACTACT7020.11642640590689868No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCGGCTCCAGCTGCT7020.11642640590689868No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATGAGCGTAGTTCCAGTT6940.11509960925838701No Hit
ATTGTCCCTCGGCCCCAGATATCAAAAGCGAAAGTAATGGAGCAGGTACC6910.11460206051519513No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAACC6800.1127777151234916No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCCCACTGCTATAATGGGT6740.11178261763710785No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCAAAGAAGGTTGCGGACGAACT6640.11012412182646826No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCTTGACCACTACTATC6610.1096265730832764No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGCATAAC6600.10946072350221245No Hit
ACTGTCCCTTGGCCCCACACATCAAGACCATCACTCCTCTCAGGCTCCAG6540.10846562601582868No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCCCGTGTAGGCCAGTCAAAAT6510.10796807727263681No Hit
AGGGTGCCACGGCCCCAGAGATCGAAGTACCAGTCTCCATCACCGTAGTC6410.10630958146199722No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGACCAGTCAAAATATCGTATGGC6390.1059778822998693No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTACCGACGATAACTCCC6370.1056461831377414No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCGAAACC6370.1056461831377414No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCAACCCGGAGTAGCAGCT6290.10431938648922973No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTGCGGAATCATAGCGAC6280.10415353690816577No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTTGATAGC6270.10398768732710181No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGAACCAGCTGCTACTATACGCCT6230.10332428900284597No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGTTGGGGGTTTTGGCCAC6210.10299258984071806No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCTGCTTCACGATAACTCCC6190.10266089067859013No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCTGTTCCCATA6170.10232919151646222No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCAAATACCGTTTGAGGGA6120.10149994361114245No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGTAAAGCAGCT6080.10083654528688661No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCTAAGTA6050.10033899654369473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)