FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969604
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC130301.3438475913878243No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA33620.3467394936489536No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGGG19460.2007004921596858No Hit
CACACCAAGCTCAGAGAGGGCAGGGGTGGGAAGGAGACATCAGATGTGGG18210.18780863115251176No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCTCAAGATAGCTCCCACCACG15750.1624374486903932No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGCGGA15590.16078729048147491No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGACGTAGT15310.15789951361586793No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCGGATTAC14300.14748288992207126No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGAGGATTGTAGCCGCTCTCGC14000.1443888432803495No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCCCCAGTTACTGTAGTCATG13860.14294495484754602No Hit
GCGGTCCCTTGGCCCCAGACGTCCAAAGCGTAGTAGTAGAAGTGTCTGAC13600.1402634477580538No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCGTAGCAGCTACCACCACT13070.134797298691012No Hit
AGGGTTCCCTGGCCCCAGTAGTCCGCGCAGTCACCACCACAATTGAGGTC12800.1320126567134624No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCTACGAGCTGCTATATTCGA12510.129021744959798No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCGGCTGCCGTAATCTCTCGC12390.1277841263031093No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTGTAATCGTA12380.12768099141505193No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAAGTCTCAACGTCACCCA12380.12768099141505193No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGAGTAGTCGTAATAACC12270.1265465076464206No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCAGAGCAGCTGGTACTACT12260.1264433727583632No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAATAAGTCCCCGAACCACCGTA12180.1256182936539041No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACTGAACCATAACCCCTGAAACTCT12070.12448380988527274No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAATGTT12050.12427754010915797No Hit
AGGGTTCCCTGGCCCCAGTAGTCATAATTGTAGCCATCTCTACCCTCTCT12040.12417440522110056No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTAGAGTAGCAGTTACCATA12010.1238650005569284No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGTAGTCTGTG11950.12324619122858405No Hit
ATTGTCCCTTGGCCCCAGAAATCAAAAGCAAACCCCGTAGTTTGGCAGCT11920.12293678656441186No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTGAATTGCCCAGTACCA11900.12273051678829706No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCCATAACTCCCTGAACCATA11770.12138976324355098No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAAAACGGGTTCTCGCACAGTAATA11760.12128662835549357No Hit
AGGGTTCCCTGGCCCCAGTAGTCCAAAGGTAGCCACTCCTCATCTCTCGC11710.12077095391520662No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCCCGAACCATAGTAAT11630.11994587481074749No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCTACCCGACGAAGGACTCCCCG11540.11901766081823095No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATTG11530.11891452593017357No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATCCGCCACTGCTATACGCTACTCT11500.11860512126600138No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATAACCTCCCGAGCCGGCCAA11480.11839885148988659No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTAACCGGT11440.11798631193765702No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCACCCCCGGAATCGTAACGTCG11430.11788317704959964No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGATTAGC11400.11757377238542745No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTTTGTCTATAGCAGCT11320.11674869328096832No Hit
GTAGTCCCTTGGCCCCAGACATCCATACCGTAGAAATAACTATTCCAAAA11280.11633615372873875No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTGGGCAG11240.11592361417650918No Hit
AGAGTTCCCTGGCCCCAGTAGCCGTAGTTCCAGTTGAAATGTCTCGCACA11160.11509853507205003No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCCCATGTC11130.11478913040787785No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGATTTCTCC11120.11468599551982046No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATACCCGGGAGATTTACCGTA11100.11447972574370568No Hit
GTGGTCCCTTGGCCCCAGACGTCCTTTCCACTGCTATACCGACGATCTCT11090.11437659085564827No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGGGGAATGACCACT11090.11437659085564827No Hit
AGGGTTCCCTGGCCCCAATAGGCAAGGTCGTACCAACTGCCGCTATACTC11060.11406718619147611No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATGGCCTATA11050.11396405130341872No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCCAGCTGCTGCTAT11040.11386091641536134No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCTCAAGTTCGG10990.11334524197507435No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCCCCCTTTGA10970.11313897219895958No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCGGTAGT10890.11231389309450043No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTGAGACACTCCAAA10880.11221075820644305No Hit
AGGGTTCCCTGGCCCCAGAGCTCCCCGAACCATAGTTTATCTCTCGCACA10870.11210762331838565No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGACGGACGAGCTGCTCTCTACTGTG10870.11210762331838565No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCCGTATAGCAGTGGCTGGTA10820.1115919488780987No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCTTCAGTCAA10780.11117940932586913No Hit
GTGGACCCCTTGCCCCAGACGTCCATGTAGTAATAAAAAGAGGATCCGCC10780.11117940932586913No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTTCCGTGGGCA10760.11097313954975432No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTTGCTTTCTCCGATAAC10750.11087000466169694No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATAT10750.11087000466169694No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCAACCGT10750.11087000466169694No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCAGCTACCACCACTACA10740.11076686977363953No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCTCTCATCACATCTCTCGC10670.1100449255572378No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCACTAATTGT10670.1100449255572378No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTAACCAC10670.1100449255572378No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATAGTTCCGGCCCCTTCTCTCGCACA10650.10983865578112302No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGAAATAGTAGACGTTGTAGCC10650.10983865578112302No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGAGAAATTGTAGCCATCTCTAGA10580.10911671156472126No Hit
AGGGTTCCCTGGCCCCAGTAGCCGACCATACCACTACTACCCGCATTTAA10570.10901357667666388No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGAGACCGAAGGAGTTACCACCGTC10560.10891044178860648No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTATGAGGCCAGCCACTGGTC10550.1088073069005491No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGAGGATAAAGTAGCAGCT10540.10870417201249169No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGTCTCC10540.10870417201249169No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGAATTCACGTA10530.10860103712443431No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTATCGGTCAGTCGTTATCTC10500.10829163246026212No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTTTCTACAATATGAGGGTG10460.10787909290803255No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGCCCATCGA10440.10767282313191777No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATGTAA10420.10746655335580298No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACTTGACGAGCTGCTATCTAGGGG10400.1072602835796882No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCCCGGCCAGTCATC10380.10705401380357342No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCAGGCCCCGTAGCTCCCACA10370.10695087891551604No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAAAAGGCC10350.10674460913940124No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCTTTGTCTCTCGCACAGTA10320.10643520447522906No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCGACAGCCACT10270.1059195300349421No Hit
AGGGTTCCCTGGCCCCAGTCGGGGCAGCTACCACCACCCTTCTCCTCTCT10270.1059195300349421No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGAGAGGGTTATAATA10260.1058163951468847No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGGGGGATCCCCCAGTTAA10250.10571326025882732No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTCAAAAGGACT10240.10561012537076991No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTATTGGATTTGAGG10230.10550699048271255No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCAGCAAACAGACTCCCT10220.10540385559465514No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTTCCCCGAACCAGCCAC10190.10509445093048297No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGACGACCATAGCTGTATCCCTTTG10180.10499131604242556No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGTTATAATAGTAGTCCCAATA10170.10488818115436818No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCCCGTCGGTAGTTACTGTAGG10160.10478504626631079No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTGCGTAGAGCCCCGAACCCGAACC10160.10478504626631079No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCCGT10130.1044756416021386No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCCACCACCCGCTTCCCAATCTCTCGC10120.10437250671408121No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTACGTTGAGGCGTGATC10110.1042693718260238No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTCCGTAACCATAGCTGTATCCAC10110.1042693718260238No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAAGGTCT10090.10406310204990903No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCCGTAAC10090.10406310204990903No Hit
TGGGGACAGGGACAGGTGGTCCTGGGATCAGGCTTCAGCTGCACGTCCTG10060.10375369738573686No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGTGATAA10040.10354742760962207No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTTGGAGAGACCAGTCAAAAT10030.10344429272156468No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGCTATAGCATACACCATT10020.1033411578335073No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGATTCCA10010.10323802294544988No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATTAGGCCCGACAGTCCGTGCCCTCT9950.10261921361710553No Hit
GTGGTCCCTTGGCCCCAGACGTCCATGCCGTAGTAGTGGTATCTACCCCT9940.10251607872904815No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTTGATACGAGAGTAGCAGCT9940.10251607872904815No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGGTGCCGGGCAGCTACC9940.10251607872904815No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAGTAGCAG9930.10241294384099074No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCCCCAGACGGGGGT9930.10241294384099074No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGAGGTTATCCGTGC9900.10210353917681858No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCTAAAACCGAAATAATAACC9900.10210353917681858No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGCG9900.10210353917681858No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAATGACCGTAGCT9870.10179413451264639No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACGGGAAAAAGAG9860.10169099962458901No Hit
AGGGTTCCCTGGCCCCAGGATACAGCATACGCCATTTCTGGCCCGCTCGC9850.10158786473653161No Hit
GTGGTCCCTTGGCCCCAGACGTCCCAACCTCTCGCACAGTAATACACAGC9820.10127846007235944No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAATTGTAGCCATCTCTACCCCAGTG9820.10127846007235944No Hit
AGGGTTCCCAGGCCCCAGTGGTCTATCAACCACCCGGCGCCCGCCGCACA9810.10117532518430204No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAAGGTTTGTAATAACCACT9790.10096905540818725No Hit
AGGGTTCCCTGGCCCCAGAAGCTTCCAAAAGTCGGATCTCTCACACAGTA9790.10096905540818725No Hit
AGGGTTCCCTGGCCCCAGAGGTCCGCCCCAAATGTTCCTCGCGCACAATA9760.10065965074401509No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAATCCCGCAGCTGGTACT9750.10055651585595768No Hit
GTGGTCCCTTGGCCCCAGACGTCCATCGGCCTAAATAACCATAGCTGTGC9740.10045338096790031No Hit
AGGGTGCCACGGCCCCAGAGATCGAAAAAGAGACCAGCCACGAAATGATC9740.10045338096790031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)