FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001132189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893734
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGTAGTCAAAAATTGTTGCGCCTGATCCTACTAG29780.33320876233868246No Hit
ATTGTCCCTTGGCCCCACACATCAAAAGCACCAAAGCCAACAAAATCTTT14290.15989097427198698No Hit
ACAGGTCTGCCGCCTTGCAATGCGACGCCTGCCTGGGAGCGCAGGTGAGG13640.15261811679985318No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATAGTCTACTATCATAGTAAT13510.15116354530542644No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCCCTCCGGAGTAGCAGCTACC13360.1494851935810879No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTCACCCGCCCATAACCACT12960.14500958898285174No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCTAAACCATAGCTGTGTCC12900.14433824829311628No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCCCAGGTTATT12850.14377879771833676No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATGCCCCGAGGGGCAGCTACC12740.14254800645382182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGGGTAAC12720.14232422622391No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACCGACCAAGGGG12700.1421004459939982No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGAACCGCGTACCAGCTGCTGC12270.13728917105089433No Hit
ATGCTTCCCTGGCCCCAGTAGTCAATGTAGTCGTAGCCATCGTGGAATCT12170.1361702699013353No Hit
AGGGTTCCCGGGCCCCAGGAGGGGTGAGGGTCCCTCGCACAGTAATAAAC12020.13449191817699674No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGCCGTAGTAATAACCACT12000.13426813794708495No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCACCTCTTCACCCG11950.1337086873723054No Hit
AGGGTTCCCCGGCCCCAGTGGTCAAAGTACTCACACGATGTTCTTGGATT11940.1335967972573495No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCCATCCCAGCCACTGGAAT11770.13169466530309912No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTGTT11700.1309114344984078No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCCGAATAATAACC11370.12721906070486297No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCTGCGCCATAATAACCACT11350.12699528047495115No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCCCACGGTCTTCCCGAACC11350.12699528047495115No Hit
AGGGTTCCCTGGCCCCAGCGGTCGAAGTCACCGCCCGGGGCTGTCGAACA11320.12665961013008345No Hit
TCACGGGGAACCCTGGATGAGAAGCTACGGTGTAAGTCCTGCCCCCTTGG11310.12654772001512754No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACTGGTTGTAGCCATCGTCTTCGCC10930.12229589564680318No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACCCCCCGTCGTTCCAGTCAAA10890.12184833518697957No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCGAGTAAACTGATC10860.12151266484211186No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTTTTTATTCCCCGAACC10840.12128888461220004No Hit
CCAGTGCAGGCCAACCCCACCTCCCTCCTCACGAGCAGGTGTGAAGAACG10820.12106510438228825No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGACCCTATAACTCCCCGAACC10760.12039376369255281No Hit
AGGGTTCCCTGGCCCCAGGAGTCAAAGTAGTAAAAGCTGCCGGCACGGGC10750.1202818735775969No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTATTCATAAGGCGTAGTTACTGT10720.1199462032327292No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTGAGTGGC10680.11949864277290559No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACCCTCCAATATGCAC10670.11938675265794968No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACGAAACCCAGGTTCCAGTTGGGGA10670.11938675265794968No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTCCTCCATAGGCAGAATCTTTC10660.11927486254299378No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGCGAGCAG10660.11927486254299378No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTACTCTGG10660.11927486254299378No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAACGTAGCTCCCACTCCTCTCT10610.11871541196821427No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGACGTCGTACCAGCTGC10600.11860352185325836No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCACACCATCACCGTAGTC10580.11837974162334654No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCATCCGAGACATCTCTCGC10570.11826785150839064No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCGGGGGGAGGATCTCGCACAGTA10570.11826785150839064No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGTCCCCGTTACCACCGT10530.11782029104856703No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAACCAAGCCTTCAGTTGTGTTC10480.1172608404737875No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTGCCTCCCGTGGGCGAACCA10440.11681328001396389No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCCGGACGAGCTGCTATAGG10360.11591815909431666No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAAAGACTCACC10330.11558248874944893No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGATGGGG10320.11547059863449305No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGCCTAGGTCGTTGGTGCTCTC10300.11524681840458123No Hit
AGGGTTCCCTGGCCCCAGTAGTCACAAAGGGACCATAGCAGCTGGTACTG10290.11513492828962532No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCAGTACCAGCCACTGCTATACCCGGT10290.11513492828962532No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAAAAGACTCCCCGATCTCTCGC10280.11502303817466943No Hit
AGGGTTCCCTGGCTCCAGTAGTCAAAGTTTCCGGTTGCTGGTGCAACTCT10270.11491114805971352No Hit
GTGGTCCCTTTGCCCCAGACGTCCATCCGACGAGCTGCTATATCTCTCGC10200.1141279172550222No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGATTATTAT10180.11390413702511037No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGCAGTAACCTTTCAC10160.11368035679519858No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACACCTATCATAGGTTTTCGATCTCT10130.11334468645033086No Hit
GTGGTCCCTTGGCCCCAGACATCCATAGCGAAATAGTAATAGTAAGTACT10120.11323279633537495No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTGACGGTACCA10040.11233767541572771No Hit
GTGGTCCCTTGGCCCCAGACGTCCATATCGTGGTAGTAGGCTAACTGACC10010.11200200507086001No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGGGGGTTAATAA10000.1118901149559041No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATTTGAATAGCAGCTGGTACT9970.11155444461103639No Hit
AGGGTTCCCTGGCCCCGGGGCTCCAACTCCCCAATAACCACTACTATCAT9970.11155444461103639No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGCCAATCGTAGCCACTATTATTCC9970.11155444461103639No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTATAGGGCACGGCAGCTACC9970.11155444461103639No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCCCGAAAGGAATAACTCCCTGAA9910.11088310392130096No Hit
AGGGTTCCCTGGCCCCAAAAGTCAAAGAAAGTTGGGCCAGCCACTGCTAT9900.11077121380634507No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTCAAACGGGAT9880.11054743357643325No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAAAAGTCACCGTAGTGCCCGAC9800.10965231265678602No Hit
GTGGTCCCTTGGCCCCAGACGTCCTGGGATCCCCCTACCGGTCCTCTCGC9800.10965231265678602No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATACTCATAGTCCCCGCGAGT9800.10965231265678602No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGAAGTAGTGGAAGCGCCT9790.10954042254183011No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCCGTAAGACGAGCTGCTATA9760.1092047521969624No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGCA9740.10898097196705062No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGGTGCTCGG9730.1088690818520947No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTACTCCCTGTAACC9720.1087571917371388No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGTA9710.10864530162218289No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTAGG9700.10853341150722698No Hit
GTGGCCCCTTGGCCCCAGACGTCCATACCGTGATAGTAGGCGTCATAGCA9690.10842152139227108No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATGGCCA9670.10819774116235926No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTTCGTAGTTGTAGTAGTATGACCG9660.10808585104740336No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGTGAGGGACGAGCTGCTAGGCC9620.10763829058757975No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGTAGCTCCCACCTTTTATTTACTTT9620.10763829058757975No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGGAGTTGTCCAGCGG9620.10763829058757975No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAACATAAC9610.10752640047262385No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTTGAAGTAGGGTCTTGT9570.10707884001280024No Hit
GTGGTCCCTTTGCCCCAGACGTCCATACCGTAGTAGTAGTCGAAAAGGCA9520.10651938943802071No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGTGCCCCTTGACCGCTGCTATACTT9500.10629560920810889No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATGAGGGGGAACCATAATAACCACT9500.10629560920810889No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTACCCCCTCTT9460.10584804874828528No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGGGGGGTA9440.10562426851837348No Hit
GTGGTCCCGTGGCCCCAGACGTCCATACCGTAGTAGTAATAACTCCCACT9430.10551237840341757No Hit
AGGGTTCCCTGGCCCCAGACCTGCTCCCACCCCCGATCTCTCGCACAGTA9380.10495292782863805No Hit
AGGGTTCCCTGGCCCCAGGGGTCGTCGGGTTGCCTCGATCCTCTCGCACA9380.10495292782863805No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATAC9370.10484103771368214No Hit
AGGGTTCCCTGGCCCCAGTACTCATATTCGTAATGACCACTACTATCATA9370.10484103771368214No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATTCCCCCTAAAACCAGT9330.10439347725385853No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAAAAGTAGTAATAACCACT9320.10428158713890263No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGCGTAGTAGTAGGGTCTCCCCGA9280.10383402667907903No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACGAGGTGAGACTACAAATCGCCCC9280.10383402667907903No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCCCCCACAAGATTGCTATAGCA9280.10383402667907903No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTCTAGGAT9280.10383402667907903No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTATATGGCTGACCGCCCCTC9250.1034983563342113No Hit
CACCAGGCCCGGGCGACCACGGTGCATGCCGTTCGGGACTCAGAATTGGC9240.10338646621925539No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCCTGTCGGAG9220.10316268598934358No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCGGGGCAGCTGGTACTAC9200.10293890575943176No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGCAGTAGGACGCCCCCCCGTT9190.10282701564447588No Hit
GTGGTCCCTTGGCCCCAGACGTCCATTCTGTCGCTATGCCCGGGCTCTGT9190.10282701564447588No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGGCTCGGGTAGGTACCA9170.10260323541456406No Hit
AGGGTTCCCTGGCCCCAGGAGACAAAGTAATAGTAGCTCCCACGTAGCGC9150.10237945518465226No Hit
GTGGTCCCTTTGCCCCAGACGTCCGAGTCGAAGTCACCGTTTAGTCTCCC9150.10237945518465226No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGATCGGCGTACCCCCCGTATCTCT9140.10226756506969635No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTCCAGAGA9140.10226756506969635No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAGTCTTTACG9120.10204378483978453No Hit
AGGGTTCCCTGGCCCCAGTAGTCCGATCCGGCGTAGCCACTATAATTAAC9100.10182000460987274No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCAGCTGGTACTACTACAATA9100.10182000460987274No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACCCTGTAGCTCCCACTATACC9080.10159622437996092No Hit
GTGGTCCCTTTGCCCCAGACGTCCATGTAGTAGTAGTAGTAACCCCCCTC9080.10159622437996092No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTCCGTATTACCACTACTATC9050.10126055403509321No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATGTATCGTAGCCACTATATCCCCC9040.1011486639201373No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCCGACCGGAGGTTATAGC9040.1011486639201373No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTATTATCCCTGAACCACCT9030.10103677380518139No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCAAGTATT9030.10103677380518139No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGCGCGGGCGGTTTGAGTAGCA9020.10092488369022551No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTGTCCGGGGGAGAACCAGTC9010.1008129935752696No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGGGTAGTAGTACCATGCCCCTTT9000.10070110346031369No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATAATAACC8990.10058921334535778No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAATATAGTAGCTCCCAAACTCGACC8960.10025354300049008No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATCACC8950.10014165288553417No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCCACTCCAAAA8950.10014165288553417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)