FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001132192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663647
Sequences flagged as poor quality0
Sequence length167
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTATGGCGAATAACC185642.7972702355318417No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTGGGAGC162702.4516045427765065No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTCATAGAGTGGCAT149172.2477310980084293No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTCCCTGTACCAGCTGCT145382.1906224242707344No Hit
AGGGTTCCCTGGCCCCAGGGGCCGAACGTTGACCTCGTTCGGAACCTCGG145152.1871567263921934No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAACTCCCT144272.173896664943863No Hit
AGGGTTCCCCGGCCCCAGGGGTCGAAGTAATTTATTTTTGAAAGTAATCT143832.1672666342196982No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGGAGCTGCTATGGTGTCG141432.1311028302697066No Hit
AGGGTTCCCTGGCCCCAGGGGCCGAACCAGCCCGATCCAAATAATTCTTC140832.122061879282209No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGTCCATGGGTCCCACTATAAA136902.0628436503140977No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCCAAGAGTAGCAGCTACCACCGACCT136522.0571177146886823No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGAAGACCCC131201.9769546159328677No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCATCCACTCGTCTTTCGC127021.9139693240532996No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGTCCAGCCACTGCTATA126001.898599707374553No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGACGTTCCAGTTATACCG124091.8698193467310182No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCGAACCAG123941.867559108984144No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAGCCCCG122591.8472169692622733No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGATCCCCG120901.8217516239808211No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAAACAGTACCAGCCACTGCTAG118031.7785057417572896No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACGGGGAGGCATTCAAAAAATCGTG113951.717027275042304No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTATTACCT110341.6626308866008588No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACGTTGCAGTAGCGTGACAGACCCTC107651.6220972896735766No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGAGGCACC106081.5984401345896238No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCCACCATACTACCAGCTGCTGCTAT105801.5942210241287915No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCGGGATCCGTA105761.5936182940629582No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCCTCCCCGTTTCGCACAGTAATATAC103581.5607695054750492No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTTTGATACTCCTTTACC100071.5078799421981866No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAATTGTAGGGGGGGGGCATACACCA98931.4907021353219407No Hit
AGGATTCCCTGTCCCCAGTAGTCCAATCTGTACCAGCCACTACTAACCGC94611.4256072882119561No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAATAAGACCCAAAAATCGTAAT94051.4171690672902912No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCCTCTAGC93251.4051144659736277No Hit
AGGGTTCCCTGGCCCCAGGGGTAAATTCACCCCCAAGTCTAAGACCCCAT92901.399840577897587No Hit
AGGGTTCCCTGGCCCCAGTAGTCTGATCCCACCCCCTGTTGGGTACAGTA89241.34469077687385No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCCTTTCCCCACATCCC87841.3235952245696883No Hit
AGGGTTCCCTGGCCCCAGTAGACGTAGGTTTGATACGGGGGTCTGTGTGC86311.300540799551569No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACCCCCATAATCGTAGCCACTATATC84101.267239963414285No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTATTGCCCCGGTTGGTAGTCACC84001.2657331382497021No Hit
AGGGTTCCCTGGCCCCAGGTGTCGAACCAGTTAAGGTAAAGACTCCAAAA83991.2655824557332436No Hit
AGGGTTCCCTGGCCCCAGTAGTCAACGAACAAACCCGTAGTCACCGTAGG79391.1962684981624268No Hit
AGGGTTCCCTGGCCCCAGTAGTCGTAGTAATAACCACTACTATCATAGTA79031.1908439275699279No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATCAGACCCACTGCTATACCCTCTCG71851.0826538807528703No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCCAGTATAATAACCACTCCAAAAATC69641.0493530446155863No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAAGTTAACCCTCCAAAAATCGTAAAC69451.0464900768028786No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATTATTCTTTCTCAATTTCTC67771.0211754140378846No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGAAAGAGCAGCT66451.0012853218653892No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGGACCGGTACCAGCTGCTGCTATA63910.9630119626849817No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTCCTGATAATAACTCCCTGA62010.9343822845579051No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGAGAGCCAGCCACTGCTCGGCTGTTC61300.9236838258893659No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTTTATAGGGTAGCAGTTACCACC60790.9159990175499927No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAACGTCCCCTAATAACCACTACTATC60300.9086155742435362No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCGCCAACCATTCGAACCGTGGG58500.8814927212810424No Hit
AGGGTTCCCTGGCCCCAGTCCTCCATAGCAGCTACCACCACTACAATATG58380.8796845310835429No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCAGCTCGGTACCAGCTGCT54680.8239319999939727No Hit
AGGGTTCCCTGGCCCCAGTAGTCATGGTAGGTATAATAACCACTCCAAAA53090.7999734798771033No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCGACGATAGCAGCTGGTACTACT51940.7826449904843991No Hit
AGGGTTCCCTGGCCCCAGTAGTCCCCGGGGTGGTAGCTCCCACTATAGAA48910.7369881879975348No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGAGGGCCGCGGACGAGCT42090.6342227117729757No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAGCAAGTGGGTAGGTCCCCGAACCCC38600.5816345135290297No Hit
ACAGGAGACTGTGACAGTGACGGTGACCACAGACAGGCCACCCAGGGCTC37670.567621039498408No Hit
AGGGTTCCCTGGCCCCAGTGGTCAACGTCCCCGATGACTGAATCCCCTAT37120.5593335010932016No Hit
AGGGTTCCCTGGCCCCAGTAGTCATACGGTTCGGTAGTCCCTGTCTGCAA36820.5548130255994527No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATAGGC32840.49484138404905015No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTAGCCCACCGTATACCGGT30350.457321437450934No Hit
AGGGTTCCCTGGCCCCAGTAGTCATACCCCCGATAATAACTCCCTGAACC26940.4059386993386544No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGAGGGAATAACCAGTCAA26550.40006208119678077No Hit
TCTCTCCAGGCTCTAAGGGAGAGCGGGTGGGAGTTGGGTGAGGAGGCTGG12070.1818737973651655No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAACCGTCAAACCGCGAGCTTATATC11950.18006560716766595No Hit
AGGGTTCCCTGGCCCCAGTAGACGGAGGTTTGATACGGGGGTCTGTGTGC8680.13079242428580254No Hit
ACAGACAGGCCACCCAGGGCTCGGGCTGGGTATGGTGGGCTCTGGTGGGA8120.12235420336413785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)