FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001132193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001132193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670057
Sequences flagged as poor quality0
Sequence length167
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGAGTTACTCCCTCCACCC51890.7744117291514006No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGTGTAGTAGAACGCTCTCGCACA9800.14625621402358308No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCGGGGGGGTATAAAGCCCACCAT9440.14088353677373716No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACCAACCGTAGGGCAGCTGGT9210.13745099297522448No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCAGTACGACCACTACTATCATA9040.13491389538501947No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATCCAAAAAA8980.13401844917671182No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTCGCCGTAGGAGGTTGCGGG8880.1325260388295324No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGACTACGGATACCAGCTGCTG8870.13237679779481448No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCCCGTCGTTCCAAACCTCTCGC8670.12939197710045564No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTGATAGCAGCTGGTAGTC8650.12909349503101977No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTATTAATAACCACTACTATCACG8640.1289442539963018No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCCCTCATAGCTATCGTCCT8560.12775032571855827No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAACGGATAACTCCCCGAACCATTCCC8550.12760108468384035No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACCGGACCCCCTTAGCTCC8540.12745184364912238No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGACGGGT8510.12700412054496857No Hit
AGGGTTCCCTGGCCCCAGTAGCCCCAGGAATTTGTACCAGCCACTGCTAC8400.1253624691630712No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAACCATAGCTGTATCCAGGCCTCGC8350.1246162639894815No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGGGTACCAGCTGCTGCTATACCCGC8340.12446702295476354No Hit
AGGGTTCCCTGGCCCCAGTAGTCAGGGGGCTCCCTATGAAAACTCCCCAA8320.12416854088532767No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACGAGTAATAACCAGTCAAAATATC8180.12207916639927648No Hit
AGGGTTCCCTGGTTGAACTCCCCCAAACGTAATCATAGCGCCGCACAGTA8160.1217806843298406No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCGGTCCCTGGGGCTGTATC8150.12163144329512265No Hit
AGGGCTCCCTGGCCCCAGTAGTCCGCTAACCCGTTTCTCGCACAATAATA8150.12163144329512265No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGGTGAGGCCAGCCACTGC8090.12073599708681501No Hit
AGGGTTCCCTGGCCCCAGTAGTCGTTCGCATAATTATACCCACCATATCT8060.12028827398266118No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCACCGGAATAGCAGTCACC8040.11998979191322529No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACTGGGCATAGCTGTATTGATC8030.11984055087850735No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCATCGAACGAACAATCCCCGAA8010.11954206880907146No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACCCTAGACGCAGCTGCTGCTA8000.11939282777435353No Hit
AGGGTTCCCTGGCCCCAGTAGTCTAGAGTTGGTACTACTACAATACCTCT7980.11909434570491764No Hit
AGGGTTCCCTGGCCCCAGTAGTGAAATTCTCCATAGGTGTATGCACGATC7950.11864662260076381No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAAGGGGGGGAGTTACCACCGTAGTT7950.11864662260076381No Hit
GTGGTCCCTTGGCCCCAGATGTCCATGCCATAGTAGGAGTAAAACGATTC7900.11790041742717411No Hit
ATTGTCCCTTGGCCCCAGATATCAAATCCACTTGTGGTCCCTCGTTCACA7880.11760193535773822No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGCCCCCGCGTCTGCTGCTATAACCC7870.11745269432302029No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAATAGAGTTACCACCGTAGCAACCTG7850.1171542122535844No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGATACTCCCCGAACC7850.1171542122535844No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACCACGACAA7850.1171542122535844No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATTAGC7800.11640800707999469No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTATGTTGTAAG7800.11640800707999469No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATTG7790.11625876604527674No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCCTCCGAACCATAGTAAAC7780.1161095250105588No Hit
AGGGTTCCCTGGCCCCAGGGGTCTCCGGGGGAACCACTACTATCATAGCG7770.11596028397584086No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTACTCTATAACCATAGC7760.11581104294112292No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTGGAAATAACCAGTCAAAATATC7740.11551256087168704No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGAGATTGGCCGAAAT7720.11521407880225115No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACAGTAGTAGTAGTAGTAACTATA7680.1146171146633794No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCACCCGAGGGAGCAGTCACCACC7670.11446787362866144No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCATCCCCATGAGATAACTC7660.1143186325939435No Hit
AGGGTTCCCTGGCCCCAGTAGTCATAAGTCGCGCTAGGACCACTACTATC7620.11372166845507173No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTAGATCCCGCACCCTGG7600.11342318638563585No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATGATGGGTCCGAACCAGTCGCTCC7560.11282622224676407No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCATAAAACCCCATGCCAGC7560.11282622224676407No Hit
AGGGTTCCCTGGCCCCAGTAGTCGACATAACTCCCCGAACCCCGATCTCT7530.11237849914261025No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTATTTGGGTGACCGGTCAA7520.1122292581078923No Hit
AGGGTTCCCAGGCCCCAGTAGTCAAAGTGTTCCTCGAACCCACCTCGTCT7520.1122292581078923No Hit
ATTGTCCCTTGGCCCCAAATATCAAAACCATCTTGTCCCCCCCCGAACCA7510.11208001707317437No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGTTGCAGCTGCTGCTATACGAAG7510.11208001707317437No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTTAGTAGC7500.11193077603845643No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAAACCCCCAGTAGGCCTCTCGCACA7500.11193077603845643No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGACATTAAGTAATAAGGC7460.11133381189958466No Hit
TGCACTCCCTGGCCCAAGTATTATCAGAGTAATAAAGGGATGTTTCGCAC7420.11073684776071291No Hit
GTGGTCCCTTGGCCCCAGACGTCCTTGCTGTATGCCCTCCCCGAATCTCT7400.110438365691277No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTCTAAGTCCCGGGAGAAACG7390.11028912465655906No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAATCGCTGCT7370.10999064258712318No Hit
AGGGTTCCCTGGCCCCAGTAGTCCTCCGCTGTACCAGCTGCTGTAACCTC7370.10999064258712318No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAACTAGC7370.10999064258712318No Hit
AGGGTTCCTGGGTGGGCCTTTTATAATAACTCCCCGAACCATATCCCAAG7370.10999064258712318No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAGGGCCATA7340.10954291948296936No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCTCCTCCCCAGTTAGAGGTTCG7330.10939367844825142No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGGCTAGCAAGGC7310.10909519637881554No Hit
AGGGTTCCCTGGCCCCAGTAGGACGACGAGTCCTAATAACCACTACTATC7300.10894595534409758No Hit
AGGGTTCCCTGGCCCCAGTAGTCCTCGTAATCGTAGCCACTATAGGCCGA7300.10894595534409758No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGAGAATTCGTAGTCACCGTGGCTCT7250.10819975017050788No Hit
AGGGTTCCCTGGCCCCAGTAGGGCTTGAGGGGGGTCCGGCCAGCCTCTCT7240.10805050913578994No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCTCAATCGTTCCAGTTGTAC7200.10745354499691817No Hit
ACTGTCCCTTGGCCCCAGATATCAAAAGCATCATTCTTGGTTTCGGCTGC7180.1071550629274823No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGCTGTAGAAGCAATTAATAAC7120.10625961671917464No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCACTGGTCATAGCAGTCACCAC7080.10566265258030287No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTTCACGCTAACCACTAC7060.105364170510867No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGCTTTTGCTGCT7050.10521492947614905No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAATCCTTTTGTAGTAACGGCTGCTGC7040.10506568844143109No Hit
ATTGTCCCTTGGCCCCAGATATCAAAAGCATCTTCGCGATAATAGAATCG7010.10461796533727727No Hit
AGGGTTCCCTGGCCCCAGGGGGAGTGATAACCACTACTATCATAGTAACA7010.10461796533727727No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGCGTTTTAA7000.10446872430255935No Hit
AGGGTTCCCTGGCCCCAGTAGCCCCAGATGTAGTAGCTCCCACTAAGCCG7000.10446872430255935No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGGGTAGCTGTATCCCTTC6990.10431948326784139No Hit
AGGGTTCCCTGGCCCCAGTAGTCAATTTCGCGTCCGGGGTAACCACTACT6980.10417024223312345No Hit
AGGGCTCCCTGGCCCCAGTAGTCAAAGTACTAGTCCCAGAAGGCCAGAAT6980.10417024223312345No Hit
GTGGTCCCTTGGCCCCAGACGTCCAAACCGTAGTAGTAGTAGTAGTTCCC6970.1040210011984055No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAGAGGGTAGTCACCGTAGTCATCCCC6970.1040210011984055No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTCCGCATAGCCACTATATTT6960.10387176016368757No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCCTTATAATAACTCCCCGAACCATA6950.10372251912896963No Hit
AGGGTTCCCTGGCCCCAGTAGCCGTAGCTCCCGGAGCTCGCACAGTAATA6930.10342403705953375No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGCCTGCTGCTGCTCGCCCTTC6920.1032747960248158No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTACCGCCTCTTGCCACTGCC6900.10297631395537993No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTACCCCGGGGTATAATAACCAGT6900.10297631395537993No Hit
AGGGTGCCCTGGCCCCAGTGCTGGAAGTCCGATAACTCCCCGATCCCTTC6890.10282707292066197No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTCTTTAACCACTACAGCGTG6870.10252859085122609No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAGTCACCGTCTAGGGAGAA6860.10237934981650815No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAACCCCCTCCTCTCGCACA6860.10237934981650815No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTACTAGGGAAT6860.10237934981650815No Hit
GTGGTCCCTTGGCCCCAGACGTCCATAGTCCTGCTGCTATAACGGGAGTC6850.1022301087817902No Hit
AGGGTTCCCTGGCCCCAGGGGTCGAACCAGTTTTGAGTCATATGGACTCT6840.10208086774707226No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGAGGTTAGTCCTCCCCGAACCATA6840.10208086774707226No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGAGTACCTTCTGTACAAGCCACT6830.10193162671235433No Hit
AGGGTGCCCTGGCCCCAGTGCTGGGGGCTGCTAGCTGCTGCTATACGTCC6830.10193162671235433No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAATCCCACTGCTATAGGTTGTCTCGC6830.10193162671235433No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAATATGGGGCCAGCCA6810.10163314464291844No Hit
AGGGTTCCCTGGCCCCAGTAGTCACCAGGGGATCAGCCACTGCTAGAGTC6810.10163314464291844No Hit
AGGGTTCCCTGGCCCCAGTAGTCGAAAGAGTAGCAGCTACCACCACTACA6800.1014839036082005No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGTAATATTC6790.10133466257348256No Hit
AGGGTTCCCTGGCCCCAGTAGTGCCCGGCCCATGAGCCATCTCCGCTCGC6790.10133466257348256No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGGGTGGGTTGGTATCATATGCGAC6770.10103618050404668No Hit
AGGGTTCCCTGGCCCCAGTACCCAAAGGACGGCTCCTTAAGGCCTCTCGC6760.10088693946932872No Hit
AGGGTTCCCTGGCCCCAGGGGCCTCCCGAACCATAGTATGAGCCCGCACA6750.10073769843461078No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAAATGGCCTG6750.10073769843461078No Hit
GTGGTCCCTTGGCCCCAGACGTCCATACCGTAGTAGTAGTAGGAACCCCA6750.10073769843461078No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTACTCATCGGCTGCTGCCAA6750.10073769843461078No Hit
AGGGTTCCCTGGCCCCAGTAGTCAAAGTAGGATAATAACCAGTCAAAATA6710.10014073429573903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)